| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Ex: 530 nm; Em 557 nm
Hypochlorous acid (HOCl) is the primary molecular target. The probe is designed for the selective detection of HOCl over other reactive oxygen species (ROS) in biological samples. |
|---|---|
| ln Vitro |
HKOCl-4 (10 μM) exhibits a greater degree of florescence with an increase in hypochlorous acid concentration[1]. The fluorescence intensity of HKOCl-4 is >20 times higher than that of other analytes (1 equiv of OH and ONOO as well as 10 equiv of O2, NO, 1O2, ROO, TBHP, and H2O2), and it responds extremely quickly to 1 equiv of HOCl (hit a plateau within 2 min)[2]. After being evaluated on RAW264.7 macrophages up to 50 μM, HKOCl-4 was found to be non-toxic in living cells[2].
HKOCl-4 is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity. It has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Its absorption/emission maxima are 530/557 nm. |
| ln Vivo |
There is no specific in vivo activity data available for this compound. However, as a fluorescent probe for reactive species, similar compounds are typically used for real-time imaging of HOCl in live cells and animal models.
|
| Enzyme Assay |
A standard protocol involves incubating HKOCl-4 (e.g., 5 uM) with varying concentrations of HOCl in a buffer solution (e.g., PBS, pH 7.4) for a short period (e.g., 5-10 minutes) at room temperature. The fluorescence intensity (Ex/Em: 530/557 nm) is then measured with a fluorescence spectrophotometer.
|
| Cell Assay |
A general protocol for cellular imaging involves seeding cells in a confocal dish and incubating with HKOCl-4 (e.g., 1-10 uM) for 20-30 minutes at 37degC. Cells are then washed to remove excess probe. Fluorescence imaging is performed with a confocal microscope using excitation at 530 nm and emission collection at 557 nm.
|
| Animal Protocol |
A typical protocol for in vivo imaging involves intravenous or intraperitoneal injection of an optimized concentration of HKOCl-4 into a live animal model. After a suitable circulation time (e.g., 30 minutes to 2 hours), the animal is placed under an in vivo imaging system to monitor HOCl-induced fluorescence.
|
| ADME/Pharmacokinetics |
General PK properties for small molecule probes are not applicable, as these compounds are typically used for acute imaging. However, formulation can be in PBS or saline with co-solvents like DMSO for in vivo use.
|
| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. For HKOCl-4, no specific toxicity studies are reported. In similar experiments, incubation with up to 10 uM for 24 hours in cell models shows good biocompatibility and no significant cytotoxicity.
|
| References | |
| Additional Infomation |
HKOCl-4 is a research tool for studying oxidative stress and inflammatory diseases. The improved pH stability over fluorescein-based probes makes it particularly suitable for imaging in acidic environments such as lysosomes or inflamed tissues. This product is for research use only.
|
| Molecular Formula |
C38H35CL2NO7
|
|---|---|
| Molecular Weight |
688.593009233475
|
| Exact Mass |
687.179
|
| CAS # |
2031170-85-9
|
| PubChem CID |
122646992
|
| Appearance |
Yellow to orange solid powder
|
| LogP |
8.6
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
48
|
| Complexity |
1180
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
N1(CCCC(OCC)=O)C2=C(C=C3C4(C5=C(C=CC=C5)C(=O)O4)C4=CC=C(OC5=CC(Cl)=C(O)C(Cl)=C5)C=C4OC3=C2)C(C)CC1(C)C
|
| InChi Key |
LUSCIMHITFMNOM-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C38H35Cl2NO7/c1-5-45-34(42)11-8-14-41-31-19-33-28(18-25(31)21(2)20-37(41,3)4)38(26-10-7-6-9-24(26)36(44)48-38)27-13-12-22(17-32(27)47-33)46-23-15-29(39)35(43)30(40)16-23/h6-7,9-10,12-13,15-19,21,43H,5,8,11,14,20H2,1-4H3
|
| Chemical Name |
ethyl 4-[9'-(3,5-dichloro-4-hydroxyphenoxy)-2',2',4'-trimethyl-3-oxospiro[2-benzofuran-1,6'-3,4-dihydrochromeno[3,2-g]quinoline]-1'-yl]butanoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 200 mg/mL (290.45 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (7.26 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4522 mL | 7.2612 mL | 14.5224 mL | |
| 5 mM | 0.2904 mL | 1.4522 mL | 2.9045 mL | |
| 10 mM | 0.1452 mL | 0.7261 mL | 1.4522 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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