| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
Biotin-C5-Azide does not target a specific protein or enzyme. The azide functional group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with alkyne-bearing molecules. The biotin moiety then serves as a high-affinity ligand for avidin, streptavidin, and neutravidin proteins.
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|---|---|
| ln Vitro |
The in vitro activity of Biotin-C5-Azide is its function as a click chemistry reagent for biotinylation. The compound contains an azide group that reacts with alkyne-containing molecules through CuAAC reactions, forming a stable triazole linkage. The biotin moiety enables the detection or purification of the labeled conjugates using streptavidin-based methods.
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| ln Vivo |
Biotin-C5-Azide is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving biotinylation and click chemistry labeling. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro labeling assays for Biotin-C5-Azide involve incubating the azide with alkyne-containing biomolecules under CuAAC reaction conditions. The reaction forms a stable triazole linkage between the azide and alkyne groups. The biotinylated product can be detected or purified using streptavidin-conjugated reagents.
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| Cell Assay |
For in vitro cellular experiments, Biotin-C5-Azide is used to label alkyne-functionalized biomolecules in cell lysates or on cell surfaces via click chemistry. The biotinylated products can be detected using fluorescently labeled streptavidin or purified using streptavidin beads.
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| Animal Protocol |
In vivo animal experiments with Biotin-C5-Azide are not typically performed, as the compound is primarily used as an in vitro labeling reagent.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Biotin-C5-Azide have not been characterized, as it is a research-use biotinylation reagent rather than a drug candidate. The compound has a molecular weight of 255.34 g/mol and appears as a white solid. It should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Biotin-C5-Azide is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
Biotin-C5-Azide (DecarboxyBiotin-N3) is a click chemistry reagent containing an azide group for CuAAC reactions with alkyne-containing molecules. It is used to prepare biotinylated conjugates for detection and purification applications and has no clinical or therapeutic applications.
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| Molecular Formula |
C10H17N5OS
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|---|---|
| Molecular Weight |
255.34
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| Exact Mass |
255.115
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| CAS # |
1260586-88-6
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| PubChem CID |
164512772
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| Appearance |
White to off-white solid powder
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| LogP |
2.1
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
17
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| Complexity |
331
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| Defined Atom Stereocenter Count |
3
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| SMILES |
N(CCCCCC1C2C(NC(=O)N2)CS1)=[N+]=[N-]
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| InChi Key |
CSDCRNNAOYVBRW-CIUDSAMLSA-N
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| InChi Code |
InChI=1S/C10H17N5OS/c11-15-12-5-3-1-2-4-8-9-7(6-17-8)13-10(16)14-9/h7-9H,1-6H2,(H2,13,14,16)/t7-,8-,9-/m0/s1
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| Chemical Name |
(3aS,4S,6aR)-4-(5-azidopentyl)-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (391.63 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.79 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.79 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.79 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9163 mL | 19.5817 mL | 39.1635 mL | |
| 5 mM | 0.7833 mL | 3.9163 mL | 7.8327 mL | |
| 10 mM | 0.3916 mL | 1.9582 mL | 3.9163 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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