| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Nucleic acids and proteins are the primary targets. The compound is used as a general fluorescent label for these biomolecules in various biochemical assays.
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|---|---|
| ln Vitro |
5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm.
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| ln Vivo |
There is no specific in vivo activity data available for this compound. This compound is a classic fluorescent dye (a rhodamine derivative) used extensively as a labeling reagent in molecular biology.
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| Enzyme Assay |
A standard protocol involves conjugating 5-Carboxytetramethylrhodamine to a target molecule (e.g., an antibody or oligonucleotide) using carbodiimide chemistry (e.g., EDC/NHS). The labeled product is then purified and analyzed by fluorescence spectroscopy (Ex/Em: 558/586 nm).
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| Cell Assay |
A general protocol for cell staining involves incubating fixed and permeabilized cells with a 5-Carboxytetramethylrhodamine-conjugated antibody or probe (e.g., 1-10 ug/mL) for 1 hour at room temperature. After washing, cells are imaged using a fluorescence microscope with a TRITC (tetramethylrhodamine) filter set.
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| Animal Protocol |
There is no standard in vivo protocol for this compound. However, 5-Carboxytetramethylrhodamine-labeled probes (e.g., antibodies or nanoparticles) can be injected intravenously into animal models for imaging studies. General guidelines for in vivo imaging should be followed, including dose optimization and circulation time.
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| ADME/Pharmacokinetics |
General PK properties for small molecule dyes are not typically applicable. In vivo, 5-Carboxytetramethylrhodamine-labeled conjugates will exhibit PK properties dictated by the conjugated carrier (e.g., antibody or nanoparticle). Free dye is likely cleared rapidly via renal filtration.
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| Toxicity/Toxicokinetics |
General toxicity for fluorescent dyes is considered low. At working concentrations (uM range), 5-Carboxytetramethylrhodamine exhibits minimal toxicity. The carboxylic acid group allows for conjugation to biomolecules with minimal impact on their function.
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| References |
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| Additional Infomation |
5-Carboxytetramethylrhodamine is a classic fluorescent dye (a rhodamine derivative) used extensively as a labeling reagent in molecular biology. It is commonly used in fluorescence microscopy, flow cytometry, and immunoassays as a red-orange fluorophore.
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| Molecular Formula |
C25H22N2O5
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|---|---|
| Molecular Weight |
430.45
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| Exact Mass |
430.152
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| CAS # |
150322-05-7
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| PubChem CID |
4612276
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| Appearance |
Brown to breen solid powder
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| LogP |
3.727
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
32
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| Complexity |
723
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN(C)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)N(C)C
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| InChi Key |
DCVXPZDNLDPUES-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H22N2O5/c1-26(2)15-6-9-19-21(12-15)31-22-13-16(27(3)4)7-10-20(22)25(19)18-8-5-14(23(28)29)11-17(18)24(30)32-25/h5-13H,1-4H3,(H,28,29)
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| Chemical Name |
3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 12.5 mg/mL (29.04 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3232 mL | 11.6158 mL | 23.2315 mL | |
| 5 mM | 0.4646 mL | 2.3232 mL | 4.6463 mL | |
| 10 mM | 0.2323 mL | 1.1616 mL | 2.3232 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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