| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| Other Sizes |
| Targets |
Bromocresol green does not target a specific protein or enzyme. Its utility as a pH indicator is based on its chemical properties as a weak acid that undergoes a color change upon protonation or deprotonation in response to changes in hydrogen ion concentration.
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|---|---|
| ln Vitro |
The in vitro activity of Bromocresol green is its function as a pH indicator. The compound exhibits a color change from yellow (λmax=435 nm) at low pH to blue-green at high pH, with a pK of 4.7. This property makes it valuable for titrations and for monitoring pH changes in growth media for microorganisms.
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| ln Vivo |
Bromocresol green is not used as a therapeutic agent. Its in vivo utility is limited to research and diagnostic applications as a pH indicator. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro binding assays are not applicable for Bromocresol green as it functions as a pH indicator rather than a drug targeting specific enzymes or receptors. The compound is used in colorimetric assays where its color change in response to pH is measured spectrophotometrically.
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| Cell Assay |
For in vitro cellular experiments, Bromocresol green is used in growth media for microorganisms to monitor pH changes. The compound can also be used in various titration applications.
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| Animal Protocol |
In vivo animal experiments with Bromocresol green are not typically performed, as the compound is primarily used as an in vitro pH indicator.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Bromocresol green have not been characterized, as the compound is a research-use pH indicator rather than a drug candidate. The compound has a molecular weight of 698.05-698.014 g/mol and is sparingly soluble in water. It should be stored at room temperature.
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| Toxicity/Toxicokinetics |
Bromocresol green has a mutagenicity classification (RTECS Class: Mutagen). The compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References |
[1]. Delanghe S, et al. Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. Clin Chem Lab Med. 2018 Feb 23;56(3):436-440.
[2]. Jurmanović S, et al. Organically modified silicate thin films doped with colourimetric pH indicators methyl red and bromocresol green as pH responsive sol–gel hybrid materials[J]. Thin Solid Films, 2010, 518(8): 2234-2240. [3]. Chaiyo S, et al. A novel paper-based colorimetry device for the determination of the albumin to creatinine ratio. Analyst. 2018 Nov 5;143(22):5453-5460. [4]. Hou H, et al. Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells. Sci Rep. 2017 May 11;7(1):1759. |
| Additional Infomation |
Bromocresolgreen belongs to the benzofuran class of compounds. It is an indicator and reagent formerly used for serum albumin determination and pH indication. See also: BCG (USP) (note moved to).
Bromocresol green is a triphenylmethane dye that serves as a pH indicator, transitioning from yellow to blue-green with a pK of 4.7. It is used in microbial growth media and titrations and has no clinical or therapeutic applications. |
| Molecular Formula |
C21H14BR4O5S
|
|---|---|
| Molecular Weight |
698.01
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| Exact Mass |
693.729
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| CAS # |
76-60-8
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| PubChem CID |
6451
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| Appearance |
Off-white to light brown solid powder
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| Density |
2.1±0.1 g/cm3
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| Boiling Point |
626.0±55.0 °C at 760 mmHg
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| Melting Point |
218.5 °C
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| Flash Point |
332.4±31.5 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.716
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| LogP |
7.8
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
|
| Heavy Atom Count |
31
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| Complexity |
742
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
BrC1C(=C(C([H])=C(C=1C([H])([H])[H])C1(C2=C([H])C(=C(C(=C2C([H])([H])[H])Br)O[H])Br)C2=C([H])C([H])=C([H])C([H])=C2S(=O)(=O)O1)Br)O[H]
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| InChi Key |
FRPHFZCDPYBUAU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3
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| Chemical Name |
2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (143.26 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4326 mL | 7.1632 mL | 14.3264 mL | |
| 5 mM | 0.2865 mL | 1.4326 mL | 2.8653 mL | |
| 10 mM | 0.1433 mL | 0.7163 mL | 1.4326 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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