| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Neutral lipids (e.g., triglycerides, lipid droplets) are the primary molecular targets. The probe is designed to specifically stain neutral lipids in live cells and tissues.
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|---|---|
| ln Vitro |
In undifferentiated 3T3-L1 cells that are differentiated by treatment with IBMX, dexamethasone, and insulin, the surface of a spherical intracellular structure is stained by LipidGreen 2 (10 mM)[1].
LipidGreen 2 is a second generation small molecule probe for lipid imaging. LipidGreen 2 has a better fluorescence signal compared with the previous LipidGreen, and selectively stains neutral lipids in cells and fat deposits in live zebrafish. |
| ln Vivo |
There is no specific in vivo activity data available for this compound. However, it is used for imaging neutral lipids and fat deposits in live zebrafish, indicating its utility as a tool for studying lipid metabolism and related disorders.
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| Enzyme Assay |
A standard protocol involves incubating LipidGreen 2 (e.g., 1-5 uM) with lipid droplets extracted from cells or with artificial lipid vesicles in a buffer solution (e.g., PBS, pH 7.4) at room temperature. The fluorescence intensity is then measured using a fluorescence spectrophotometer.
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| Cell Assay |
A general protocol involves seeding cells in a confocal dish and incubating with LipidGreen 2 (e.g., 1-10 uM) for 10-30 minutes at 37degC. After washing, lipid droplets are visualized using a confocal microscope. Co-staining with other organelle trackers can confirm specificity.
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| Animal Protocol |
A typical protocol for in vivo imaging involves adding LipidGreen 2 to the water of live zebrafish embryos or larvae at a specific concentration (e.g., 1-10 uM) and incubating for a defined period (e.g., 30 minutes to 2 hours). The fish are then mounted and imaged under a fluorescence microscope to visualize fat deposits.
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| ADME/Pharmacokinetics |
General PK properties for small molecule probes are not applicable, as these compounds are typically used for acute imaging. However, formulation can be in PBS or saline with co-solvents like DMSO for in vivo use.
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| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. For LipidGreen 2, no specific toxicity studies are reported. In similar experiments, incubation with up to 10 uM for 24 hours in cell models shows good biocompatibility and no significant cytotoxicity.
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| References | |
| Additional Infomation |
LipidGreen 2 is a research tool for studying obesity, metabolic disorders, lipid storage diseases, and adipogenesis. Its compatibility with fluorescence microscopy and flow cytometry makes it a powerful tool for lipidomics and cellular metabolic studies.
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| Molecular Formula |
C22H29NO4
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|---|---|
| Molecular Weight |
371.469966650009
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| Exact Mass |
371.209
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| CAS # |
1382764-52-4
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| PubChem CID |
142712987
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| Appearance |
Yellow to brown viscous liquid
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| LogP |
5.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
27
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| Complexity |
616
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCOC(=O)C1(C(=O)C2=C(N1CC=C(C)C)C=CC(=C2)OC)CC=C(C)C
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| InChi Key |
VFAFYBVPGKPJDO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H29NO4/c1-7-27-21(25)22(12-10-15(2)3)20(24)18-14-17(26-6)8-9-19(18)23(22)13-11-16(4)5/h8-11,14H,7,12-13H2,1-6H3
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| Chemical Name |
ethyl 5-methoxy-1,2-bis(3-methylbut-2-enyl)-3-oxoindole-2-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (269.20 mM)
H2O: < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.73 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6920 mL | 13.4600 mL | 26.9201 mL | |
| 5 mM | 0.5384 mL | 2.6920 mL | 5.3840 mL | |
| 10 mM | 0.2692 mL | 1.3460 mL | 2.6920 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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