| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Ex: 490 nm; Em 527 nm
HKOCl-3 targets hypochlorous acid (HOCl), a reactive oxygen species (ROS) produced by myeloperoxidase in immune cells. The compound does not target a specific protein or enzyme but rather serves as a chemical probe that selectively reacts with HOCl, resulting in a fluorescence turn-on response. |
|---|---|
| ln Vitro |
HKOCl-3 (10 μM) exhibits a greater degree of florescence with an increase in hypochlorous acid concentration[1]. When HKOCl-3 (10 μM) is treated, the compound's fluorescence intensity increases significantly more than when other ROS (10 μM) and RNS (100 μM) are treated [1].
The in vitro activity of HKOCl-3 is its function as a fluorescent probe for detecting hypochlorous acid. The compound exhibits low background fluorescence in the absence of HOCl. Upon reaction with HOCl, the fluorescence intensity increases in a concentration-dependent manner. The probe provides excellent selectivity for HOCl over other reactive oxygen species and offers a rapid response. |
| ln Vivo |
HKOCl-3 is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the detection of hypochlorous acid in biological systems, oxidative stress research, and disease mechanism studies. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro assays for HKOCl-3 involve incubating the fluorescent probe with samples containing hypochlorous acid in appropriate buffer conditions. The fluorescence intensity is measured using a fluorescence spectrophotometer or microplate reader with excitation at 490 nm and emission at 527 nm. The increase in fluorescence is proportional to the concentration of HOCl in the sample.
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| Cell Assay |
For in vitro cellular experiments, HKOCl-3 is dissolved in DMSO at 90 mg/mL and diluted in cell culture medium. Cells are incubated with the probe at concentrations such as 10 μM, and the fluorescence is measured using fluorescence microscopy or flow cytometry. The increase in fluorescence indicates the presence of HOCl in the cellular environment.
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| Animal Protocol |
In vivo animal experiments with HKOCl-3 are not typically performed, as the compound is primarily used as a research tool for in vitro detection of HOCl. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of HKOCl-3 have not been characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound has a molecular weight of 493.29 g/mol and should be stored at -80°C, protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for HKOCl-3 has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid, with excitation at 490 nm and emission at 527 nm. It is widely used in oxidative stress research and disease mechanism studies and has no clinical or therapeutic applications.
|
| Molecular Formula |
C26H14CL2O6
|
|---|---|
| Molecular Weight |
493.29176568985
|
| Exact Mass |
492.016
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| CAS # |
2031170-80-4
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| PubChem CID |
122646982
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| Appearance |
Yellow to orange solid powder
|
| LogP |
6.2
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
34
|
| Complexity |
770
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(OC1=CC(Cl)=C(O)C(Cl)=C1)=C3)C1=C(C=CC=C1)C(=O)O2
|
| InChi Key |
OWWYBZBGVRLHND-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C26H14Cl2O6/c27-20-10-15(11-21(28)24(20)30)32-14-6-8-19-23(12-14)33-22-9-13(29)5-7-18(22)26(19)17-4-2-1-3-16(17)25(31)34-26/h1-12,29-30H
|
| Chemical Name |
3'-(3,5-dichloro-4-hydroxyphenoxy)-6'-hydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (202.72 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (10.14 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0272 mL | 10.1360 mL | 20.2721 mL | |
| 5 mM | 0.4054 mL | 2.0272 mL | 4.0544 mL | |
| 10 mM | 0.2027 mL | 1.0136 mL | 2.0272 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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