| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
Methionine sulfoxide reductase (Msr) enzymes are the primary molecular targets. Msr-blue is a turn-on probe that is specifically reduced by Msr enzymes, resulting in a substantial fluorescence increase. It is used to monitor Msr activity in live cells.
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| ln Vitro |
Methionine sulfoxide reductase A (Msr A) activates Msr-blue, which releases blue fluorescence. More than a 100-fold increase in emission is seen when Msr-blue is exposed to Msr A, and this response is time- and dose-dependent. Either the purified Msr A or a cell lysate catalyzes the conversion of Msr-blue to its corresponding sulfide (15′)[1]. Msr-blue is used to examine the function of Msrs in 6-OHDA-treated PC12 cells, which are used as a cellular model of Parkinson's disease (PD). The Msr activity in a PD model was shown to be declining for the first time with the help of Msr-blue[1].
In cell-free assays, Msr-blue serves as a substrate for methionine sulfoxide reductase. Upon reduction by Msr in the presence of thioredoxin/NADPH, Msr-blue is converted to a highly fluorescent product, with over 100-fold fluorescence increase. The activity is measured by fluorescence intensity (λex=340 nm, λem=440 nm). |
| ln Vivo |
There is no specific in vivo activity data available for this compound. Msr-blue is used for monitoring methionine sulfoxide reductase activity in live cells (e.g., PC12 cells) to examine Msr function in models of Parkinson‘s disease and other neurodegenerative conditions.
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| Enzyme Assay |
A standard protocol involves incubating Msr-blue (e.g., 10-50 uM) with varying concentrations of purified methionine sulfoxide reductase, thioredoxin, and NADPH in a buffer solution (e.g., 50 mM Tris-HCl, pH 7.4) at 37degC. The fluorescence intensity (Ex/Em: 340/440 nm) is measured over time with a fluorescence spectrophotometer. The increase in fluorescence is correlated with Msr enzymatic activity.
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| Cell Assay |
A general protocol for cellular imaging involves seeding cells (e.g., PC12 cells used as a cellular model of Parkinson‘s disease) in a confocal dish and incubating with Msr-blue (e.g., 10-50 uM) for 30-60 minutes at 37degC. After washing, live-cell imaging is performed using a fluorescence microscope with excitation at 340 nm and emission collection at 440 nm. Cells can be treated with oxidative stress inducers (e.g., 6-hydroxydopamine, 6-OHDA) to assess the effect on Msr activity.
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| Animal Protocol |
A typical protocol for in vivo imaging would involve intravenous or intraperitoneal injection of Msr-blue into a live animal model. After a suitable circulation time (e.g., 30 minutes to 2 hours), the animal is placed under an in vivo imaging system with appropriate filters (Ex/Em: 340/440 nm) to monitor Msr activity. However, UV excitation is less common for in vivo imaging.
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| ADME/Pharmacokinetics |
General PK properties for small molecule probes are not applicable, as these compounds are typically used for acute imaging. For in vivo use, formulation can be in PBS or saline with co-solvents like DMSO.
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| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. No specific toxicity studies are reported for Msr-blue. Standard safety precautions for handling laboratory chemicals should be observed.
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| References | |
| Additional Infomation |
Msr-blue is a research tool for studying redox biology, oxidative damage, and neurodegenerative diseases. Methionine sulfoxide reductase (Msr) is an important antioxidant enzyme that repairs oxidative damage to proteins. By blocking Msr function, Msr-blue can sensitize cells to oxidative stress, making it valuable for investigating oxidative damage pathways and age-related diseases. This product is for research use only.
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| Exact Mass |
222.035
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|---|---|
| CAS # |
2966537-39-1
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| PubChem CID |
162642075
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| Appearance |
White to yellow solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
335
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)S(=O)C
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| InChi Key |
IFTHUDRVKVIXDN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H10O3S/c1-7-5-11(12)14-10-6-8(15(2)13)3-4-9(7)10/h3-6H,1-2H3
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| Chemical Name |
4-methyl-7-methylsulfinylchromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (112.48 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (11.25 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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