| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
4-Methylumbelliferyl-α-D-galactopyranoside targets α-galactosidase (α-Gal A), an enzyme that hydrolyzes the glycosidic bond between galactose and various substrates. The compound serves as a fluorogenic substrate for this enzyme, releasing a fluorescent product upon enzymatic cleavage.
|
|---|---|
| ln Vitro |
The wavelength of emission for 4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal) is 440 nm, while the wavelength of excitation is 365 nm [1].
The in vitro activity of this compound is its function as a fluorogenic substrate for α-galactosidase. Upon enzymatic cleavage by α-galactosidase, the substrate releases 4-methylumbelliferone (4-MU), a blue fluorescent molecule. The fluorescence can be quantified, enabling the sensitive and quantitative measurement of α-galactosidase activity. The compound is used to characterize novel α-galactosidases and to study enzyme function. |
| ln Vivo |
4-Methylumbelliferyl-α-D-galactopyranoside is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the measurement of α-galactosidase activity in biological samples. The compound is not intended for systemic therapeutic use.
|
| Enzyme Assay |
In vitro enzyme assays for this compound involve incubating the fluorogenic substrate with α-galactosidase enzyme preparations in appropriate buffer conditions. The enzyme cleaves the substrate, releasing 4-methylumbelliferone (4-MU), which is measured fluorimetrically. The fluorescence intensity is proportional to α-galactosidase activity.
|
| Cell Assay |
For in vitro cellular experiments, this compound is typically used in cell-free systems for enzyme activity assays. The substrate can be added to cell lysates or purified enzyme preparations to assess α-galactosidase activity. The fluorogenic readout provides a sensitive and quantitative measure of enzyme activity.
|
| Animal Protocol |
In vivo animal experiments with this compound are not typically performed, as the compound is used as an in vitro enzyme substrate. However, the substrate can be used to measure α-galactosidase activity in samples collected from animal models.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of this compound have not been characterized, as it is a research-use enzyme substrate rather than a drug candidate. The compound has a molecular weight of 338.31-338.312 g/mol and should be stored under appropriate conditions, protected from light.
|
| Toxicity/Toxicokinetics |
Toxicological data for this compound has not been systematically evaluated, as it is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
|
| References | |
| Additional Infomation |
4-Methylumbelliferone α-D-galactoside is an α-D-galactoside with a 4-methylumbelliferone substituent attached to its anomeric carbon atom. It is a chromogenic compound belonging to the coumarin class, and is an α-D-galactoside and monosaccharide derivative. Its function is similar to that of 4-methylumbelliferone.
4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal) is a fluorogenic substrate for α-galactosidase that releases blue fluorescent 4-MU upon cleavage. It is used to characterize novel α-galactosidases and has no clinical or therapeutic applications. |
| Molecular Formula |
C16H18O8
|
|---|---|
| Molecular Weight |
338.31
|
| Exact Mass |
338.1
|
| CAS # |
38597-12-5
|
| PubChem CID |
2733788
|
| Appearance |
White to off-white solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
626.9±55.0 °C at 760 mmHg
|
| Melting Point |
171-173ºC
|
| Flash Point |
233.9±25.0 °C
|
| Vapour Pressure |
0.0±1.9 mmHg at 25°C
|
| Index of Refraction |
1.641
|
| LogP |
-0.46
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
24
|
| Complexity |
506
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)O[C@@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
|
| InChi Key |
YUDPTGPSBJVHCN-CHUNWDLHSA-N
|
| InChi Code |
InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1
|
| Chemical Name |
4-methyl-7-[(2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 33.33 mg/mL (98.52 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9559 mL | 14.7793 mL | 29.5587 mL | |
| 5 mM | 0.5912 mL | 2.9559 mL | 5.9117 mL | |
| 10 mM | 0.2956 mL | 1.4779 mL | 2.9559 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
