| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
4-Methylumbelliferyl oleate targets lipases, specifically acid and alkaline lipases. The compound serves as a fluorogenic substrate for these enzymes, releasing a fluorescent product upon enzymatic cleavage of the ester bond between the oleate moiety and the 4-methylumbelliferone fluorophore.
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|---|---|
| ln Vitro |
4-Methylumbelliferyl (4-MU) oleate was used as a substrate to measure the inhibitory activity against pancreatic lipase. The composition of the reaction mixture was as follows: 5 µL of sample solution, 20 µL of McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 50 µL of 0.1 mM 4-MU oleate. The reaction mixture was mixed with 25 µL of porcine pancreatic lipase, and the final volume was adjusted to 0.1 ml. Using a fluorescence multi-detection reader set to an excitation wavelength of 320 nm and an emission wavelength of 450 nm, the amount of 4-MU released by the lipase was measured after the mixture was incubated at 37 °C for 10 minutes[1].
The in vitro activity of 4-Methylumbelliferyl oleate is its function as a fluorogenic substrate for lipases. Upon enzymatic cleavage by lipases, the substrate releases 4-methylumbelliferone (4-MU), a fluorescent molecule with an emission maximum at 445-454 nm. The increase in fluorescence over time is proportional to lipase activity, enabling the sensitive and quantitative measurement of lipase activity in biological samples. The compound is used to continuously follow the hydrolytic activity of carboxylesterases. |
| ln Vivo |
4-Methylumbelliferyl oleate is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the measurement of lipase activity in biological samples. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for 4-Methylumbelliferyl oleate involve incubating the fluorogenic substrate with lipase enzyme preparations in appropriate buffer conditions at physiological pH. The enzyme cleaves the substrate, releasing 4-methylumbelliferone (4-MU), which is measured fluorimetrically with excitation around 360 nm and emission at 445-454 nm. The fluorescence intensity is proportional to lipase activity.
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| Cell Assay |
For in vitro cellular experiments, 4-Methylumbelliferyl oleate is typically used in cell-free systems for enzyme activity assays. The substrate can be added to cell lysates or purified enzyme preparations to assess lipase activity. The fluorogenic readout provides a sensitive and quantitative measure of lipase activity.
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| Animal Protocol |
In vivo animal experiments with 4-Methylumbelliferyl oleate are not typically performed, as the compound is used as an in vitro enzyme substrate. However, the substrate can be used to measure lipase activity in samples collected from animal models.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 4-Methylumbelliferyl oleate have not been characterized, as it is a research-use enzyme substrate rather than a drug candidate. The compound should be stored at +4°C, protected from light. It has an emission maximum of 449 nm.
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| Toxicity/Toxicokinetics |
Toxicological data for 4-Methylumbelliferyl oleate has not been systematically evaluated, as it is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References |
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| Additional Infomation |
4-Methylumbelliferyl oleate (4-MU oleate) is a fluorogenic substrate for acid and alkaline lipases. Upon cleavage by lipases, it releases fluorescent 4-methylumbelliferone. It is used to continuously monitor carboxylesterase hydrolytic activity and has no clinical or therapeutic applications.
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| Molecular Formula |
C28H40O4
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|---|---|
| Molecular Weight |
440.61
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| Exact Mass |
440.293
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| CAS # |
18323-58-5
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| PubChem CID |
6436487
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| Appearance |
White to off-white solid-liquid Mixture
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| Density |
1.023g/cm3
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| Boiling Point |
571.5ºC at 760mmHg
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| Melting Point |
37-38ºC(lit.)
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| Flash Point |
281.3ºC
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| Vapour Pressure |
4.53E-13mmHg at 25°C
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| Index of Refraction |
1.515
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| LogP |
8.044
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
17
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| Heavy Atom Count |
32
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| Complexity |
607
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCC/C=C\CCCCCCCC(=O)OC1=CC2=C(C=C1)C(=CC(=O)O2)C
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| InChi Key |
NKQFKJYKCVDLPT-KHPPLWFESA-N
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| InChi Code |
InChI=1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10-
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| Chemical Name |
(4-methyl-2-oxochromen-7-yl) (Z)-octadec-9-enoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (226.96 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (5.67 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2696 mL | 11.3479 mL | 22.6958 mL | |
| 5 mM | 0.4539 mL | 2.2696 mL | 4.5392 mL | |
| 10 mM | 0.2270 mL | 1.1348 mL | 2.2696 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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