| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
| Targets |
AQC targets primary and secondary amines (-NH₂ and -NH-) on amino acids, peptides, proteins, and polyamines. The compound does not target a specific protein or enzyme but rather serves as a chemical derivatization reagent that reacts with amines to form fluorescent derivatives. The N-hydroxysuccinimidyl carbamate group reacts with amines to form stable urea linkages, attaching the fluorescent 6-aminoquinolyl moiety to the amine-containing molecule.
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|---|---|
| ln Vitro |
Many of the drawbacks associated with other deriving reagents are overcome when using AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate), which reacts with primary and secondary amino acids to yield fluorescent derivates (λ excitation and emission at 250 and 395 nm, respectively). This allows amino acid detection at under-picomolar levels.
The in vitro activity of AQC is its function as a derivatization reagent for amino acid analysis. The compound reacts with primary and secondary amino acids to yield fluorescent derivatives, allowing amino acid detection at sub-picomolar levels. The derivatives can be separated by HPLC and detected by fluorescence, providing sensitive and quantitative analysis of amino acids in biological samples. The compound is a reactive probe for the pre-column derivatization of amines. |
| ln Vivo |
AQC is not used as a therapeutic agent. Its in vivo utility is limited to research and analytical applications involving amino acid analysis. The compound is not intended for systemic therapeutic use.
|
| Enzyme Assay |
In vitro derivatization assays for AQC involve incubating the reagent with amino acid samples in appropriate buffer conditions. The reaction forms fluorescent derivatives that can be analyzed by HPLC with fluorescence detection. The derivatives are stable and provide sensitive detection of amino acids at sub-picomolar levels. The method is widely used for amino acid analysis in biological samples.
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| Cell Assay |
For in vitro cellular experiments, AQC is not typically used in cell-based assays. The compound is used for the analysis of amino acids in biological samples such as cell lysates, plasma, or tissue extracts.
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| Animal Protocol |
In vivo animal experiments with AQC are not typically performed, as the compound is used as an analytical reagent rather than a therapeutic candidate.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of AQC have not been characterized, as the compound is a research-use analytical reagent rather than a drug candidate. The compound has a molecular weight of 285.25 g/mol and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
AQC is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References |
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| Additional Infomation |
The structure given in the first document
AQC (6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate) (CAS#: 148757-94-2) is a reagent for amino acid analysis by HPLC with fluorescence detection. It reacts with primary and secondary amino acids to yield fluorescent derivatives. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C14H11N3O4
|
|---|---|
| Molecular Weight |
285.25
|
| Exact Mass |
315.073
|
| CAS # |
148757-94-2
|
| PubChem CID |
2762553
|
| Appearance |
Off-white to yellow solid powder
|
| Boiling Point |
661.0±65.0 °C at 760 mmHg
|
| Flash Point |
353.5±34.3 °C
|
| Vapour Pressure |
0.0±2.1 mmHg at 25°C
|
| LogP |
-1.98
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
21
|
| Complexity |
438
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1CC(=O)N(C1=O)OC(=O)NC2=CC3=C(C=C2)N=CC=C3
|
| InChi Key |
LINZYZMEBMKKIT-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H11N3O4/c18-12-5-6-13(19)17(12)21-14(20)16-10-3-4-11-9(8-10)2-1-7-15-11/h1-4,7-8H,5-6H2,(H,16,20)
|
| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) N-quinolin-6-ylcarbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (350.57 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.76 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.76 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5057 mL | 17.5285 mL | 35.0570 mL | |
| 5 mM | 0.7011 mL | 3.5057 mL | 7.0114 mL | |
| 10 mM | 0.3506 mL | 1.7528 mL | 3.5057 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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