| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
1. Preparation of DiSC3 (5) membrane staining solution:
1.1 Preparation of DMSO or EtOH stock solution: The stock solution should be prepared in DMSO or EtOH to a concentration of 1-5 mM. Note 1): The unused portion of the stock solution should be stored at -20 ℃. Avoid repeated freezing/thawing cycles. 1.2 Preparation of working solution: Dilute the stock solution into a suitable buffer solution, such as serum-free medium, HBSS, or PBS, to prepare a working solution of 1 to 5 uM. Note 2): For different cell types and/or experimental conditions, the concentration of the working solution should be determined based on your specific needs. 2. Stain the cells into a suspension: 2.1 The cell density suspended in the dye working solution is 1 × 10~6/mL. 2.2 Incubate at 37 ° C for 2-20 minutes. The incubation time depends on the cell types. Firstly, incubate for 20 minutes, and then optimize as needed to obtain uniform labeling. 2.3 Centrifuge the marked suspension tube at 1000 to 1500rpm for 5 minutes. 2.4 Remove the supernatant and gently resuspend the cells in preheated (37 ° C) growth medium. Wash twice according to steps 2.3 and 2.4. 3. Stained adherent cells: 3.1 Culture adherent cells on sterile glass coverslips. 3.2 Remove the cover glass from the growth medium and gently drain excess medium. Place the cover glass in the humidity chamber. 3.3 Transfer 100 μ L of dye working solution to the corner of a cover glass and gently stir until all cells are covered. 3.4 Incubate the cover glass at 37 ° C for 2-20 minutes. The cultivation time varies depending on the cell type. Start incubating for 20 minutes, then optimize as needed to obtain uniform labeling. 3.5 Discharge the dye working solution and clean the cover glass with growth medium two to three times. For each washing cycle, cover the cells with preheated growth medium, incubate for 5-10 minutes, and then drain the medium. 4. Microscopic examination. 5. Flow cytometry detection: Cells labeled with DiS can be analyzed using a conventional FL3 flow cytometer. |
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| Enzyme Assay |
3,3'-Dipropyl-2,2'-thiadicarbocyanine iodide [DiS-C(3)(5)], often used as a tracer dye to assess the mitochondrial membrane potential, was investigated in detail regarding its effects on the structure and function of isolated mitochondria. As reported previously, DiS-C(3)(5) had an inhibitory effect on NADH-driven mitochondrial electron transfer. On the contrary, in the presence of inorganic phosphate, DiS-C(3)(5) showed dose-dependent biphasic effects on mitochondria energized by succinate. At higher concentrations, such as 50 micro m, DiS-C(3)(5) accelerated mitochondrial oxygen consumption. Measurements of the permeability of DiS-C(3)(5)-treated mitochondrial membranes to poly(ethylene glycol) and analysis of mitochondrial configuration by transmission electron microscopy revealed that the accelerating effect of DiS-C(3)(5) on mitochondrial oxygen consumption reflects the induction of the mitochondrial permeability transition (PT). When the mitochondrial PT was induced by DiS-C(3)(5), release of mitochondrial cytochrome c was observed, as in the case of the PT induced by Ca(2+). On the contrary, at a low concentration such as 5 micro m, DiS-C(3)(5) showed an inhibitory effect on the latent oxygen consumption by mitochondria. This effect was shown to reflect inhibition of the PT induced by a low concentration of Ca(2+). Furthermore, in the absence of inorganic phosphate, DiS-C(3)(5) caused mitochondrial swelling. Under this condition, DiS-C(3)(5) caused changes in the membrane status of the mitochondria, but did not induce a release of mitochondrial cytochrome c [1].
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| References |
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| Additional Infomation |
See also: 3,3'-Dipropyl-2,2'-thiadicarbocyanine (preferred).
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| Molecular Formula |
C25H27IN2S2
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|---|---|
| Molecular Weight |
546.53
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| Exact Mass |
546.066
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| Elemental Analysis |
C, 54.94; H, 4.98; I, 23.22; N, 5.13; S, 11.73
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| CAS # |
53213-94-8
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| PubChem CID |
5706741
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| Appearance |
Blue to dark blue solid powder
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| Melting Point |
245ºC (dec.)(lit.)
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| LogP |
3.36
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
30
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| Complexity |
564
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCN\1C2=CC=CC=C2S/C1=C/C=C/C=C/C3=[N+](C4=CC=CC=C4S3)CCC.[I-]
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| InChi Key |
GDEURKKLNUGTDA-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C25H27N2S2.HI/c1-3-18-26-20-12-8-10-14-22(20)28-24(26)16-6-5-7-17-25-27(19-4-2)21-13-9-11-15-23(21)29-25;/h5-17H,3-4,18-19H2,1-2H3;1H/q+1;/p-1
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| Chemical Name |
(2E)-3-propyl-2-[(2E,4E)-5-(3-propyl-1,3-benzothiazol-3-ium-2-yl)penta-2,4-dienylidene]-1,3-benzothiazole;iodide
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| Synonyms |
3,3'-Dipropylthiadicarbocyanine iodide; 53213-94-8; Disc3(5); Dptdci; Dis C3-(5); 3,3'-Dipropylthiodicarbocyanine iodide; 3,3'-dipropyl-2,2'-thiadicarbocyanine; Di-S-C3-5; NK 2251;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 16.67 mg/mL (30.50 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (2.29 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (2.29 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8297 mL | 9.1486 mL | 18.2973 mL | |
| 5 mM | 0.3659 mL | 1.8297 mL | 3.6595 mL | |
| 10 mM | 0.1830 mL | 0.9149 mL | 1.8297 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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