| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
HBC620 targets the Pepper RNA aptamer, a fluorogenic RNA aptamer that specifically binds HBC and its analogs. The compound does not target a protein or enzyme but rather serves as a fluorescent ligand that becomes fluorescent upon binding to its cognate RNA aptamer. Upon forming a tight complex with the Pepper RNA aptamer, HBC620 emits strong fluorescence, allowing for the visualization of RNA dynamics in live cells.
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|---|---|
| ln Vitro |
HBCs can have their fluorescence triggered by pepper. When exposed to 1 μM HBC, Pepper-expressing mammalian cells glow intensely yellow-green [1]. Peppers minimize the disruption of target RNA translation, localization, and transcription while enabling straightforward and reliable imaging of many RNA species in living cells [1].
The in vitro activity of HBC620 is its function as a fluorogenic ligand for the Pepper RNA aptamer. The compound is nonfluorescent in solution. Upon binding to the Pepper RNA aptamer, it emits strong fluorescence. The fluorescence can be detected and quantified, enabling the imaging of RNA in living cells. The HBC-Pepper complex can be used to visualize RNA dynamics in live cells. |
| ln Vivo |
HBC620 is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the imaging of RNA in living cells using the Pepper aptamer system. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro binding assays for HBC620 involve incubating the fluorogenic ligand with the Pepper RNA aptamer in appropriate buffer conditions. The binding affinity can be determined by measuring the increase in fluorescence upon titration of HBC620 into aptamer-containing solutions. The fluorescence enhancement is proportional to the amount of bound HBC620.
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| Cell Assay |
For in vitro cellular experiments, cells expressing the Pepper RNA aptamer are incubated with HBC620, which is taken up by cells and binds to the aptamer. The resulting fluorescence can be detected using fluorescence microscopy, allowing the visualization and tracking of RNA in living cells.
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| Animal Protocol |
In vivo animal experiments with HBC620 are not typically performed, as the compound is primarily used as a research tool for imaging RNA in cell culture systems.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of HBC620 have not been characterized, as the compound is a research-use fluorogenic ligand rather than a drug candidate. The compound has a molecular weight of 365.47 g/mol and should be stored under appropriate conditions, protected from light.
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| Toxicity/Toxicokinetics |
HBC620 is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
HBC620 (CAS#: 2530162-07-1) is a non-fluorescent HBC mimetic that emits strong fluorescence upon forming a tight complex with the Pepper RNA aptamer. It is used to visualize RNA dynamics in live cells. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C19H15N3OS2
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|---|---|
| Molecular Weight |
365.47190117836
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| Exact Mass |
365.065
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| CAS # |
2530162-07-1
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| PubChem CID |
146047141
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| Appearance |
Red to reddish brown solid powder
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| LogP |
4
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
25
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| Complexity |
596
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(C1SC2C=C(N(C)CCO)SC=2C=1)=C(C1C=CC(C#N)=CC=1)C#N
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| InChi Key |
RIGJCKIIBUZTCX-OVCLIPMQSA-N
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| InChi Code |
InChI=1S/C19H15N3OS2/c1-22(6-7-23)19-10-18-17(25-19)9-16(24-18)8-15(12-21)14-4-2-13(11-20)3-5-14/h2-5,8-10,23H,6-7H2,1H3/b15-8+
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| Chemical Name |
4-[(Z)-1-cyano-2-[5-[2-hydroxyethyl(methyl)amino]thieno[3,2-b]thiophen-2-yl]ethenyl]benzonitrile
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| Synonyms |
HBC620; 2530162-07-1; (Z)-4-(1-Cyano-2-(5-((2-hydroxyethyl)(methyl)amino)thieno[3,2-b]thiophen-2-yl)vinyl)benzonitrile; 4-[(~{Z})-1-cyano-2-[5-[2-hydroxyethyl(methyl)amino]thieno[3,2-b]thiophen-2-yl]ethenyl]benzenecarbonitrile; 4-[(Z)-1-cyano-2-[5-[2-hydroxyethyl(methyl)amino]thieno[3,2-b]thiophen-2-yl]ethenyl]benzonitrile; ..
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 16.67 mg/mL (45.61 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (5.69 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7362 mL | 13.6810 mL | 27.3620 mL | |
| 5 mM | 0.5472 mL | 2.7362 mL | 5.4724 mL | |
| 10 mM | 0.2736 mL | 1.3681 mL | 2.7362 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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