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| Other Sizes |
| Targets |
p-Nitrophenyl α-D-mannopyranoside does not have a defined pharmacological target as it is a chromogenic substrate and biochemical reagent. The compound serves as a substrate for various glycosidases, enabling researchers to study enzyme kinetics and mechanisms in carbohydrate metabolism. It can be used to determine glycosidase activity and characterize the sugar recognition properties of lectins (such as Concanavalin A).
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| ln Vitro |
In vitro activity of p-Nitrophenyl α-D-mannopyranoside is assessed in enzyme activity assays. The compound serves as a chromogenic substrate for the detection of α-mannosidase activity. Upon enzymatic hydrolysis, the substrate releases p-nitrophenol, which gives a yellow solution that can be detected spectrophotometrically. The compound is used in biochemical enzyme assays and in vitro diagnostic analysis to study enzyme kinetics and mechanisms.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for p-Nitrophenyl α-D-mannopyranoside as it is a chromogenic substrate and research reagent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro biochemical and diagnostic research. The compound is for research use only and not intended for therapeutic use.
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| Enzyme Assay |
For non-cellular assays, p-Nitrophenyl α-D-mannopyranoside is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound can be evaluated as a substrate in enzyme assays for α-mannosidase activity. Typical protocols involve incubating the substrate with enzyme samples in appropriate buffer, then measuring the release of p-nitrophenol spectrophotometrically at appropriate wavelength. Purity is typically ≥95% to ≥98%. Molecular weight: 301.25.
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| Cell Assay |
For in vitro cell-based studies, p-Nitrophenyl α-D-mannopyranoside is not typically used as a direct test compound in cell culture. The compound is used in biochemical enzyme assays and diagnostic applications. If handled in a biological laboratory context, standard safety precautions should be taken. The compound should be stored in a cool, dry place protected from light and moisture.
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| Animal Protocol |
For in vivo animal studies, p-Nitrophenyl α-D-mannopyranoside is not typically administered as a test compound. The compound is used primarily in in vitro biochemical and diagnostic research. If used in animal studies for specific research purposes, it would be administered via appropriate routes with suitable formulations. Standard animal welfare guidelines should be followed.
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| ADME/Pharmacokinetics |
p-Nitrophenyl α-D-mannopyranoside has a molecular weight of 301.25 and molecular formula C12H15NO8. Purity: typically ≥95% to ≥98%. Storage: 2-8°C refrigerator or -20°C in tightly closed containers. Shipping conditions: ambient temperature. The compound is a chromogenic substrate that gives a yellow solution upon hydrolysis.
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| Toxicity/Toxicokinetics |
p-Nitrophenyl α-D-mannopyranoside is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| Additional Infomation |
p-Nitrophenyl α-D-mannopyranoside (CAS 10357-27-4) is also known as 4-nitrophenyl α-D-mannopyranoside. It is used as a chromogenic substrate for the detection of α-mannosidase activity, for the study of enzyme kinetics and mechanisms in carbohydrate metabolism, and to characterize the sugar recognition properties of lectins such as Concanavalin A. Applications include biochemical enzyme assays and in vitro diagnostic analysis. No clinical trials or therapeutic approvals exist.
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| Molecular Formula |
C12H15NO8
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|---|---|
| Molecular Weight |
301.25
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| Exact Mass |
301.079
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| CAS # |
10357-27-4
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| PubChem CID |
449023
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
582.2±50.0 °C at 760 mmHg
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| Melting Point |
179-181ºC
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| Flash Point |
305.9±30.1 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.648
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| LogP |
-0.55
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
354
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O
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| InChi Key |
IFBHRQDFSNCLOZ-GCHJQGSQSA-N
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| InChi Code |
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11+,12+/m1/s1
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| Chemical Name |
(2R,3S,4S,5S,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (331.95 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3195 mL | 16.5975 mL | 33.1950 mL | |
| 5 mM | 0.6639 mL | 3.3195 mL | 6.6390 mL | |
| 10 mM | 0.3320 mL | 1.6598 mL | 3.3195 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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