| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| Other Sizes |
| Targets |
4-Aminoantipyrine hydrochloride does not have a defined pharmacological target as it is a biochemical reagent and colorimetric assay reagent. The compound is known for its analgesic, anti-inflammatory, and antipyretic properties. It is used as a reagent in clinical chemistry assays and diagnostic tests to measure blood glucose levels, peroxidase activity, and various enzymatic reactions. As a peroxidase-mediated chromogenic reagent, it is used for the quantitative analysis of glucose, cholesterol, uric acid, and other biomolecules.
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|---|---|
| ln Vitro |
In vitro activity of 4-Aminoantipyrine hydrochloride is assessed in colorimetric assays. The compound serves as a substrate for peroxidase enzymes and exhibits strong reactivity in colorimetric assays for the detection and quantification of various compounds, particularly in the analysis of glucose levels. It is widely utilized in enzymatic and biochemical research applications, facilitating studies in metabolic pathways and enzymatic activity. The compound forms colored complexes with phenolic compounds.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for 4-Aminoantipyrine hydrochloride as a therapeutic agent. The compound is used primarily as a biochemical reagent and diagnostic assay reagent. It is not administered to animals for pharmacological evaluation. Its applications are limited to clinical chemistry, biochemical research, and diagnostic test development.
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| Enzyme Assay |
For non-cellular assays, 4-Aminoantipyrine hydrochloride is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound can be evaluated in colorimetric assays for the determination of various substances. Typical protocols involve mixing the compound with peroxidase, hydrogen peroxide, and the analyte to be measured, then detecting the colored product spectrophotometrically. Purity: ≥95%.
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| Cell Assay |
For in vitro cell-based studies, 4-Aminoantipyrine hydrochloride is not typically used as a direct test compound. The compound is used as a reagent in biochemical and enzymatic assays. If handled in a biological laboratory context, standard safety precautions should be taken. The compound should be stored in a cool, dry place protected from light and moisture.
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| Animal Protocol |
For in vivo animal studies, 4-Aminoantipyrine hydrochloride is not typically administered as a test compound. The compound is used primarily in in vitro diagnostic and biochemical research applications. If used in animal studies for specific research purposes, it would be administered via appropriate routes with suitable formulations. Standard animal welfare guidelines should be followed.
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| ADME/Pharmacokinetics |
4-Aminoantipyrine hydrochloride has a molecular weight of 239.70 and molecular formula C11H14ClN3O. Purity: ≥95%. Storage: keep in a cool, dry place protected from light and moisture. The compound is a solid at room temperature. Solubility: soluble in water and aqueous buffers. The compound is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
4-Aminoantipyrine hydrochloride is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| Additional Infomation |
4-Aminoantipyrine hydrochloride (CAS 22198-72-7) is also known as Ampyrone HCl and 4-aminoantipyrine HCl. It is used as a reagent in colorimetric assays for the determination of various substances, in clinical chemistry assays to measure blood glucose levels, peroxidase activity, and enzymatic reactions, and in the development of diagnostic test kits. No clinical trials or therapeutic approvals exist.
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| Molecular Formula |
C11H14CLN3O
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|---|---|
| Molecular Weight |
239.70
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| Exact Mass |
239.082
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| CAS # |
22198-72-7
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| PubChem CID |
72639
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| Appearance |
Light yellow to light brown solid powder
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| Boiling Point |
309ºC at 760 mmHg
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| Melting Point |
220-225 °C
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| Flash Point |
140.7ºC
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| Vapour Pressure |
0.000659mmHg at 25°C
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| LogP |
2.449
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
16
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| Complexity |
305
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=C(C(=O)N(N1C)C2=CC=CC=C2)N.Cl
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| InChi Key |
UZSCVCWALGRUTR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H13N3O.ClH/c1-8-10(12)11(15)14(13(8)2)9-6-4-3-5-7-9;/h3-7H,12H2,1-2H3;1H
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| Chemical Name |
4-amino-1,5-dimethyl-2-phenylpyrazol-3-one;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~208.6 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (20.86 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1719 mL | 20.8594 mL | 41.7188 mL | |
| 5 mM | 0.8344 mL | 4.1719 mL | 8.3438 mL | |
| 10 mM | 0.4172 mL | 2.0859 mL | 4.1719 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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