| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
ARC12 targets regulatory pathways involved in secondary metabolism in S. coelicolor. It modulates the production of various secondary metabolites, including antibiotics, by influencing signaling cascades that control gene expression of biosynthetic gene clusters. The specific molecular target within the bacterial signaling network has not been fully elucidated.
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| ln Vitro |
In cell-free assays, ARC12 shows no direct antibacterial activity. Its biological activity is assessed by its ability to alter the metabolic profile of S. coelicolor cultures. The compound specifically decreases the production of germicidin (a polyketide antibiotic) while modulating the levels of other secondary metabolites, including actinorhodin and undecylprodigiosin, in a concentration-dependent manner.
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| ln Vivo |
There is no direct in vivo activity reported for ARC12 in mammalian systems. It is a bacterial secondary metabolism probe used exclusively in microbiological research. The compound is under investigation for its ability to control cellular functions linked to bacterial signaling and may have potential applications in discovering new antibiotics by activating silent biosynthetic gene clusters.
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| Enzyme Assay |
A typical protocol involves culturing Streptomyces coelicolor in liquid or solid media. ARC12 is added to the culture at a final concentration of 10-100 uM (from a DMSO stock) at the time of inoculation or during exponential growth. After 3-7 days of incubation, secondary metabolites are extracted from the culture broth with an organic solvent (e.g., ethyl acetate) and analyzed by HPLC-UV or LC-MS. Peak areas for germicidin, actinorhodin, and other metabolites are compared to DMSO-treated controls.
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| Cell Assay |
This compound is not used in mammalian cell experiments. Its use is strictly limited to bacterial cultures. For cellular studies in S. coelicolor, cells are grown in liquid medium in shake flasks or in 24-well plates. ARC12 is added, and the culture is incubated at 30degC with shaking for 3-7 days. The pH, growth (OD600), and metabolite production are monitored daily. Control cultures receive an equivalent volume of DMSO (final DMSO ≤1%).
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| Animal Protocol |
There is no standard in vivo animal protocol for ARC12. The compound is not administered to animals. It is a tool for bacterial microbiology and secondary metabolism research, not for in vivo pharmacological studies.
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| ADME/Pharmacokinetics |
PK properties are not applicable for this compound as it is not used as a drug. ARC12 (molecular formula C22H18N2O2, MW 342.39) is soluble in DMSO (100 mg/mL, need ultrasonic assistance). For long-term storage, stock solutions are kept at -20degC. Stability in aqueous solution is limited; the compound should be stored as a solid at -20degC.
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| Toxicity/Toxicokinetics |
General toxicity of ARC12 has not been reported. As a small molecule probe for bacteria, it is expected to have low acute toxicity to mammalian cells at the concentrations used (10-100 uM). Standard laboratory safety precautions for handling organic compounds (gloves, goggles) should be followed. The compound is for research use only.
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| Additional Infomation |
ARC12 is a secondary metabolism probe used in chemical biology to investigate the regulation of antibiotic production in Streptomyces, an important bacterial genus that produces the majority of clinically used antibiotics. By modulating germicidin production, ARC12 helps researchers understand the signaling pathways that control secondary metabolite biosynthesis, potentially leading to the discovery of new antibiotics by activating cryptic biosynthetic gene clusters.
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| Molecular Formula |
C22H18N2O2
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|---|---|
| Molecular Weight |
342.39
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| Exact Mass |
342.137
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| CAS # |
64433-38-1
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| PubChem CID |
2853037
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
4.241
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
512
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1C(N(N=C1C2=CC=CC=C2)C3=CC=C(C=C3)C(=O)O)C4=CC=CC=C4
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| InChi Key |
LRYRYLONUXSKTL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H18N2O2/c25-22(26)18-11-13-19(14-12-18)24-21(17-9-5-2-6-10-17)15-20(23-24)16-7-3-1-4-8-16/h1-14,21H,15H2,(H,25,26)
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| Chemical Name |
4-(3,5-diphenyl-3,4-dihydropyrazol-2-yl)benzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (292.06 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9206 mL | 14.6032 mL | 29.2065 mL | |
| 5 mM | 0.5841 mL | 2.9206 mL | 5.8413 mL | |
| 10 mM | 0.2921 mL | 1.4603 mL | 2.9206 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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