| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
ADA does not have a defined pharmacological target as it is a biological buffer and biochemical reagent. As a Good's buffer, ADA exhibits high water-solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. It can also serve as a chelator.
|
|---|---|
| ln Vitro |
No specific in vitro pharmacological activity data are available for ADA as it is a biological buffer and biochemical reagent. In research settings, the compound is used to maintain pH in biochemical and molecular biology applications. Its activity is assessed in terms of buffering capacity and compatibility with biological systems rather than direct biological activity.
|
| ln Vivo |
No specific in vivo pharmacological activity data have been documented for ADA as a therapeutic agent. The compound is used as a biological buffer and biochemical reagent. It is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro biochemical and molecular biology research.
|
| Enzyme Assay |
For non-cellular assays, ADA is characterized by standard analytical techniques including HPLC and titration to confirm identity and purity. The compound can be evaluated for its buffering capacity and pKa. Typical protocols involve dissolving the compound in aqueous buffers and measuring pH. pKa at 20°C: 6.9. Purity: ≥99.0%. Heavy metals: ≤5ppm.
|
| Cell Assay |
For in vitro cell-based studies, ADA is used as a buffer component in cell culture media and biochemical assays. The compound is typically dissolved in aqueous buffers and added to media at appropriate concentrations. Cells are cultured under standard conditions in ADA-buffered media. The buffer helps maintain physiological pH and prevents protein oxidation and irreversible denaturation during gel electrophoresis.
|
| Animal Protocol |
For in vivo animal studies, ADA can be formulated using standard injection vehicles. The compound is a solid at room temperature. Dosing solutions should be freshly prepared and administered according to specific protocols with appropriate control groups. The compound is used as a buffer component in various formulations.
|
| ADME/Pharmacokinetics |
ADA has a molecular weight of 190.15 and molecular formula C6H10N2O5. It has a density of 1.522. pKa at 20°C: 6.9. Storage: powder at -20°C for 3 years; in solvent at -80°C for 1 year. Solubility: soluble in water. Purity: ≥99.0%.
|
| Toxicity/Toxicokinetics |
ADA is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
|
| References |
[1]. T Minami, et al. Identification of metallothionein isoforms with capillary zone electrophoresis using a polyacrylamide-coated tube. J Chromatogr B Biomed Appl. 1996 Oct 25;685(2):353-9.
[2]. Birte M Gerken, et al. Tweezing-adsorptive bubble separation. Analytical method for the selective and high enrichment of metalloenzymes. Anal Chem. 2005 Oct 1;77(19):6113-7. |
| Additional Infomation |
2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid is a tricarboxylic acid amide, belonging to the Goodyear buffer group, with a pKa of 6.6 at 20 °C. It is a dicarboxylic acid, tricarboxylic acid amide, and ADA. Functionally, it is associated with hypozinotriacetic acid. It is the conjugate acid of 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid ester (1-).
ADA (CAS 26239-55-4) is also known as N-(2-Acetamido)-2-iminodiacetic acid and ADA buffer. It is a zwitterionic biological buffer developed as one of the Good's buffers. Applications include biochemical and molecular biology research, preparation of immobilized pH gradients, and as a buffer component in various assays. No clinical trials or therapeutic approvals exist. |
| Molecular Formula |
C6H10N2O5
|
|---|---|
| Molecular Weight |
190.1540
|
| Exact Mass |
190.058
|
| CAS # |
26239-55-4
|
| PubChem CID |
117765
|
| Appearance |
White to off-white solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
524.9±45.0 °C at 760 mmHg
|
| Melting Point |
219 °C (dec.)(lit.)
|
| Flash Point |
271.3±28.7 °C
|
| Vapour Pressure |
0.0±3.0 mmHg at 25°C
|
| Index of Refraction |
1.558
|
| LogP |
-3.9
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
13
|
| Complexity |
211
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(C(=O)N)N(CC(=O)O)CC(=O)O
|
| InChi Key |
QZTKDVCDBIDYMD-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
|
| Chemical Name |
2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 5 mg/mL (26.30 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (52.59 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2590 mL | 26.2950 mL | 52.5901 mL | |
| 5 mM | 1.0518 mL | 5.2590 mL | 10.5180 mL | |
| 10 mM | 0.5259 mL | 2.6295 mL | 5.2590 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Link: https://clinicaltrials.gov/ct2/show/NCT02557399
Conditions:Acne VulgarisProducts are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
