| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
||
| 10g |
|
||
| 25g |
|
||
| Other Sizes |
| Targets |
2,5-Dioxopyrrolidin-1-yl acrylate does not have a defined pharmacological target as it is a protein crosslinker and biochemical reagent. The compound can react with monoclonal antibodies to modify lysine residues. This interaction suggests that this compound may play a role in protein modification and potentially influence biochemical reactions involving these proteins.
|
|---|---|
| ln Vitro |
In vitro activity of 2,5-Dioxopyrrolidin-1-yl acrylate is assessed in protein modification studies. The compound can react with a monoclonal anti-horseradish peroxidase IgG antibody to modify lysine residues. This property makes it useful for protein crosslinking and bioconjugation applications in biochemical research.
|
| ln Vivo |
No specific in vivo pharmacological activity data have been documented for 2,5-Dioxopyrrolidin-1-yl acrylate as a therapeutic agent. The compound is used as a protein crosslinker and biochemical reagent. It is not administered to animals for pharmacological evaluation. Its applications are limited to protein modification and bioconjugation research.
|
| Enzyme Assay |
For non-cellular assays, 2,5-Dioxopyrrolidin-1-yl acrylate is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound can be evaluated as a protein crosslinker in biochemical assays. Typical protocols involve incubating the compound with protein samples and analyzing crosslinking efficiency. Purity: 99.58%.
|
| Cell Assay |
For in vitro cell-based studies, 2,5-Dioxopyrrolidin-1-yl acrylate is not typically used as a direct test compound. The compound is used in protein modification and bioconjugation applications. If handled in a biological laboratory context, standard safety precautions should be taken. The compound is soluble in DMSO.
|
| Animal Protocol |
For in vivo animal studies, 2,5-Dioxopyrrolidin-1-yl acrylate is not typically administered as a test compound. The compound is used as a biochemical reagent and protein crosslinker. If used in animal studies for specific research purposes, it would be administered via appropriate routes with suitable formulations.
|
| ADME/Pharmacokinetics |
2,5-Dioxopyrrolidin-1-yl acrylate has a molecular weight of 169.13 and molecular formula C7H7NO4. It is soluble in DMSO at 100 mg/mL. Storage: -20°C, stored under nitrogen. The compound is a solid at room temperature. Purity: 99.58%.
|
| Toxicity/Toxicokinetics |
2,5-Dioxopyrrolidin-1-yl acrylate is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
|
| References |
[1]. Okyem S, et, al. High-Affinity Points of Interaction on Antibody Allow Synthesis of Stable and Highly Functional Antibody-Gold Nanoparticle Conjugates. Bioconjug Chem. 2021 Aug 18;32(8):1753-1762.
|
| Additional Infomation |
It binds to alkaline phosphatase to form a soluble, temperature-sensitive polymer-coupled enzyme.
2,5-Dioxopyrrolidin-1-yl acrylate (CAS 38862-24-7) is also known as N-Succinimidyl acrylate and N-acryloyloxysuccinimide. It is a protein crosslinker that can react with monoclonal antibodies to modify lysine residues. The compound is used in protein modification and bioconjugation research. No clinical trials or therapeutic approvals exist. |
| Molecular Formula |
C7H7NO4
|
|---|---|
| Molecular Weight |
169.13
|
| Exact Mass |
169.037
|
| CAS # |
38862-24-7
|
| Related CAS # |
37017-08-6
|
| PubChem CID |
181508
|
| Appearance |
Off-white to light yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
251.3±23.0 °C at 760 mmHg
|
| Melting Point |
69 °C
|
| Flash Point |
105.8±22.6 °C
|
| Vapour Pressure |
0.0±0.5 mmHg at 25°C
|
| Index of Refraction |
1.520
|
| LogP |
-1.21
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
12
|
| Complexity |
242
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C=CC(ON1C(CCC1=O)=O)=O
|
| InChi Key |
YXMISKNUHHOXFT-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H7NO4/c1-2-7(11)12-8-5(9)3-4-6(8)10/h2H,1,3-4H2
|
| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) prop-2-enoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (591.26 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.78 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.78 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.78 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9126 mL | 29.5631 mL | 59.1261 mL | |
| 5 mM | 1.1825 mL | 5.9126 mL | 11.8252 mL | |
| 10 mM | 0.5913 mL | 2.9563 mL | 5.9126 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
