| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| Other Sizes |
| Targets |
5,6-O-Isopropylidene-L-ascorbic acid does not have a defined pharmacological target as it is a chemically protected derivative of vitamin C used as a research reagent and cosmetic ingredient. By virtue of its profound antioxidative attributes, it orchestrates a substantial reduction in melanin synthesis while concurrently fostering collagen synthesis. As a ketal-protected derivative, it provides access to the vitamin C molecule and is primarily used in research focused on cosmetic formulations as a stable derivative of vitamin C. Its mechanism of action is chemical rather than pharmacological, serving as a precursor to retinoic acid.
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| ln Vitro |
No specific in vitro pharmacological activity data are available for 5,6-O-Isopropylidene-L-ascorbic acid as a drug candidate. In research settings, the compound is evaluated for its antioxidative properties and its ability to reduce melanin synthesis while fostering collagen synthesis. Its activity is assessed in terms of chemical stability and antioxidant potential rather than direct pharmacological activity against biological targets. The compound serves as a stable derivative of vitamin C, enhancing skin care products by providing antioxidant properties and promoting collagen synthesis, which helps in reducing signs of aging.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for 5,6-O-Isopropylidene-L-ascorbic acid as a therapeutic agent. The compound is used primarily as a research reagent and cosmetic ingredient. It is not administered to animals for pharmacological evaluation in a therapeutic context. Its applications are limited to in vitro biochemical research and cosmetic formulation studies. The compound holds immense potential in addressing an array of dermatological concerns such as skin hyperpigmentation, photoaging, and oxidative stress-related disorders.
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| Enzyme Assay |
For non-cellular assays, 5,6-O-Isopropylidene-L-ascorbic acid is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound can be evaluated for its antioxidant capacity using standard assays such as DPPH radical scavenging or metal chelation assays. Typical protocols involve dissolving the compound in appropriate solvents and analyzing its chemical stability and reactivity. Purity is typically ≥98%. Storage: powder at -20°C for up to 3 years or at 4°C for up to 2 years; in solvent at -80°C for 6 months or -20°C for 1 month.
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| Cell Assay |
For in vitro cell-based studies, 5,6-O-Isopropylidene-L-ascorbic acid can be used to assess effects on melanin synthesis and collagen production in skin cell models such as melanocytes and fibroblasts. Cells are cultured under standard conditions and treated with the compound to evaluate its antioxidative effects and impact on pigment production. The compound is typically dissolved in aqueous buffers or DMSO and diluted in cell culture medium. Appropriate controls including untreated cells should be included in the experimental design.
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| Animal Protocol |
For in vivo animal studies, 5,6-O-Isopropylidene-L-ascorbic acid can be formulated using vehicles such as DMSO:Tween 80:Saline (10:5:85) or DMSO:PEG300:Tween 80:Saline (10:40:5:45) for intravenous, intraperitoneal, intramuscular, or subcutaneous administration. Dosing solutions should be freshly prepared and administered according to specific protocols with appropriate control groups. The compound is stable at ambient temperature for a few days during shipping. Standard animal welfare guidelines should be followed for all in vivo studies.
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| ADME/Pharmacokinetics |
5,6-O-Isopropylidene-L-ascorbic acid has a molecular weight of 250.26 g/mol and molecular formula C₉H₁₄O₆. It appears as a white crystalline powder that is soluble in water. Storage: powder at -20°C for up to 3 years or at 4°C for up to 2 years; in solvent at -80°C for 6 months or -20°C for 1 month. Shipping: ambient temperature. The compound is a ketal-protected derivative of L-ascorbic acid featuring an isopropylidene bridge across the C-5 and C-6 hydroxyl positions.
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| Toxicity/Toxicokinetics |
5,6-O-Isopropylidene-L-ascorbic acid is for research use only and not for human therapeutic or diagnostic use. Standard laboratory safety practices should be followed when handling this chemical. Specific LD₅₀ values and detailed toxicological profiles have not been extensively reported for this compound. Appropriate personal protective equipment including gloves and safety glasses should be worn when handling the compound. The compound should be stored in a sealed container away from moisture and incompatible materials.
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| References | |
| Additional Infomation |
5,6-Isopropylidene-L-ascorbic acid is a ketal.
5,6-O-Isopropylidene-L-ascorbic acid (CAS 15042-01-0) is also known as L-ascorbic acid 5,6-acetonide and L-Ascorbic acid acetonide. It is a chemically protected derivative of L-ascorbic acid that is widely utilized in research focused on cosmetic formulations, serving as a stable derivative of vitamin C by providing antioxidant properties and promoting collagen synthesis. The compound is a precursor to retinoic acid and hydrogen chloride. No clinical trials or regulatory approvals for human use exist, as the product is exclusively for research purposes. |
| Molecular Formula |
C9H12O6
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|---|---|
| Molecular Weight |
216.19
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| Exact Mass |
216.063
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| CAS # |
15042-01-0
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| PubChem CID |
54691418
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| Appearance |
White to off-white solid
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
395.2±42.0 °C at 760 mmHg
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| Melting Point |
217 °C (dec.)
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| Flash Point |
161.0±21.4 °C
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| Vapour Pressure |
0.0±2.1 mmHg at 25°C
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| Index of Refraction |
1.580
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| LogP |
-0.02
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
332
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| Defined Atom Stereocenter Count |
2
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| SMILES |
O1C(C([H])([H])[H])(C([H])([H])[H])OC([H])([H])[C@@]1([H])[C@]1([H])C(=C(C(=O)O1)O[H])O[H]
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| InChi Key |
POXUQBFHDHCZAD-MHTLYPKNSA-N
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| InChi Code |
InChI=1S/C9H12O6/c1-9(2)13-3-4(15-9)7-5(10)6(11)8(12)14-7/h4,7,10-11H,3H2,1-2H3/t4-,7+/m0/s1
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| Chemical Name |
(2R)-2-[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]-3,4-dihydroxy-2H-furan-5-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~462.6 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (23.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (23.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 5 mg/mL (23.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6256 mL | 23.1278 mL | 46.2556 mL | |
| 5 mM | 0.9251 mL | 4.6256 mL | 9.2511 mL | |
| 10 mM | 0.4626 mL | 2.3128 mL | 4.6256 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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