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| Targets |
2,6-Dimethoxyphenol does not have specific biological receptors as its primary targets, but it is used as a substrate of laccases catalysis to determine enzyme activity in aqueous solution. As a plant metabolite, it may interact with various plant enzymes and signaling pathways. The compound's phenolic structure allows it to act as an antioxidant and participate in redox reactions. In laccase assays, it serves as a chromogenic substrate, where oxidation by laccase produces colored products that can be measured spectrophotometrically.
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| ln Vitro |
In vitro, 2,6-dimethoxyphenol is used as a substrate of laccases catalysis to determine enzyme activity in aqueous solution. It is used as an analytical standard for HPLC and gas chromatography. The compound serves as a potential marker compound for emission of smoke in the atmosphere from wood. It is used in the flavor and fragrance industry as a flavoring agent. The compound's ability to act as a substrate for laccases makes it valuable for studying enzyme kinetics and for developing biosensors.
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| ln Vivo |
In vivo data for 2,6-dimethoxyphenol is limited, as it is primarily a research reagent and flavoring agent. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. As a plant metabolite, it occurs naturally in various plants and contributes to the flavor and aroma of foods and beverages. The compound is used as a flavoring agent in food products at low concentrations. Specific in vivo data for therapeutic applications is not available in the public literature.
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| Enzyme Assay |
In vitro enzyme assays for 2,6-dimethoxyphenol typically evaluate its activity as a substrate for laccases. A standard assay protocol involves incubating the compound with laccase enzyme in appropriate buffer systems (e.g., acetate buffer, pH 4.5-5.5) at room temperature or 37°C. The compound is dissolved in buffer or organic solvent and diluted to working concentrations (typically 0.1-10 mM). Enzyme activity is measured by monitoring the absorbance change at a specific wavelength (e.g., 468 nm for the oxidized product) over time using a spectrophotometer. The reaction rate is calculated from the linear portion of the absorbance vs. time curve.
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| Cell Assay |
Cellular assays for 2,6-dimethoxyphenol are not typically performed, as it is primarily used as an enzyme substrate and analytical standard. The compound's antioxidant properties can be evaluated in cell-based assays by measuring its ability to scavenge reactive oxygen species or protect cells from oxidative stress. A standard protocol involves culturing appropriate cell lines in growth medium at 37°C with 5% CO₂. Cells are treated with varying concentrations of the compound. Oxidative stress is induced using H₂O₂ or other oxidants. Cellular ROS levels are measured using fluorescent probes such as DCFH-DA. Cell viability is assessed using MTT or similar assays.
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| Animal Protocol |
In vivo animal studies for 2,6-dimethoxyphenol are not standard, as it is primarily a research reagent and flavoring agent. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. Any animal studies would be limited to toxicological evaluations or studies on its metabolism as a food additive. The compound's primary value lies in its use as an analytical standard, enzyme substrate, and flavoring agent. Specific in vivo protocols are not available in the public literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 2,6-dimethoxyphenol is limited, as it is primarily a research reagent. The compound has a molecular weight of 154.16 g/mol and a molecular formula of C₈H₁₀O₃. It is a solid at room temperature with a melting point of 50-57°C and a boiling point of 261°C. The compound is stored at -20°C for long-term stability. As a phenolic compound, it may undergo metabolic conjugation (glucuronidation, sulfation) and oxidation. Specific ADME data is not available.
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| Toxicity/Toxicokinetics |
2,6-Dimethoxyphenol is classified as a hazardous substance with signal word "Warning". Hazard classifications include Acute Tox. 4 Oral, Eye Irrit. 2, Skin Irrit. 2, and STOT SE 3 (target organs: Respiratory system). The compound is classified as a combustible solid (Storage Class 11) with a WGK of 3. Standard safety precautions include handling with appropriate personal protective equipment in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is limited.
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| References | |
| Additional Infomation |
2,6-Dimethoxyphenol belongs to the phenolic class of compounds, with its structure consisting of phenol replaced by methoxy groups at the 2 and 6 positions. It is a plant metabolite. It is both a phenolic compound and a dimethoxybenzene compound. 2,6-Dimethoxyphenol has been reported to exist in ginseng (Panax japonicus), pilosa (Mucuna birdwoodiana), and other organisms with relevant data.
2,6-Dimethoxyphenol (Syringol, CAS 91-10-1) is a biochemical reagent with the molecular formula C₈H₁₀O₃. It is a plant metabolite and a volatile flavor constituent in various foods and beverages. The compound is used as a substrate of laccases catalysis and as an analytical standard for HPLC and GC. It is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. The compound is available from multiple commercial suppliers in various pack sizes. No clinical trials or approved drug status exist for this compound. |
| Molecular Formula |
C8H10O3
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|---|---|
| Molecular Weight |
154.16
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| Exact Mass |
154.062
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| CAS # |
91-10-1
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| Related CAS # |
2,6-Dimethoxyphenol-d3;2484091-60-1
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| PubChem CID |
7041
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| Appearance |
Off-white to gray solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
264.5±0.0 °C at 760 mmHg
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| Melting Point |
55 - 56 °C
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| Flash Point |
99.9±21.8 °C
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| Vapour Pressure |
0.0±0.5 mmHg at 25°C
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| Index of Refraction |
1.523
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| LogP |
0.77
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
11
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| Complexity |
104
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=C(C(=CC=C1)OC)O
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| InChi Key |
KLIDCXVFHGNTTM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H10O3/c1-10-6-4-3-5-7(11-2)8(6)9/h3-5,9H,1-2H3
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| Chemical Name |
2,6-dimethoxyphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 25 mg/mL (162.17 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 6.67 mg/mL (43.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.4868 mL | 32.4338 mL | 64.8677 mL | |
| 5 mM | 1.2974 mL | 6.4868 mL | 12.9735 mL | |
| 10 mM | 0.6487 mL | 3.2434 mL | 6.4868 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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