| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Ferene disodium does not have a defined biological target as it is a colorimetric reagent rather than a pharmacologically active compound. Its function is chemical—it serves as a chelating agent for the detection and quantification of iron (Fe²⁺) in biological and environmental samples. The compound forms a stable, blue-colored complex with ferrous iron, which can be measured spectrophotometrically. In biological systems, iron is an essential element involved in oxygen transport, electron transfer, and enzyme function. However, ferene disodium is not used therapeutically; it is a diagnostic and analytical reagent. The compound itself is not evaluated for biological activity against specific targets.
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| ln Vitro |
In vitro, ferene disodium exhibits no pharmacological activity as it is a colorimetric reagent. Its utility is demonstrated in the sensitive and selective determination of iron (Fe²⁺) in serum, plasma, water, and other samples. The compound forms a blue complex with Fe²⁺ with a high molar absorptivity, enabling detection at nanomolar concentrations. The reagent is used in clinical chemistry for serum iron determination and in environmental monitoring. In cell-based assays, the compound is not tested for biological activity. Its role is strictly analytical—providing a chromogenic reagent for iron quantification in research and clinical laboratories.
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| ln Vivo |
Ferene disodium is not a pharmacologically active agent and does not exhibit in vivo therapeutic activity. It is used as a colorimetric reagent for iron determination in biological and environmental samples. The compound is not intended for human or animal exposure as a therapeutic agent. It is used in research and clinical laboratories for diagnostic testing. No therapeutic efficacy has been reported for this compound. The compound is not administered to animals in pharmacological studies and has no known physiological effects. Its role is strictly analytical—detecting and quantifying iron in samples.
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| Enzyme Assay |
In vitro assays for ferene disodium focus on its use as a colorimetric reagent for iron determination. A standard protocol involves preparing a reagent solution (typically 0.1-1 mM in buffer at pH 4-6) and mixing with the sample containing Fe²⁺. The blue complex formed (ferene-Fe²⁺) is measured spectrophotometrically at 593 nm. The assay is linear over a range of iron concentrations (typically 0-100 µM). For serum iron determination, proteins are precipitated with acid, and the supernatant is mixed with the reagent. Quality control includes the use of iron standards and blanks. The compound is characterized by HPLC and purity analysis (>98%).
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| Cell Assay |
Cell-based experiments are not performed with ferene disodium, as it is a colorimetric reagent rather than a test compound for biological activity. The compound is used exclusively for iron determination in biological fluids, environmental samples, and research applications. No cytotoxicity or cell viability studies are conducted with this compound as it is not intended for biological applications. The reagent is used in biochemical and clinical chemistry laboratories for diagnostic testing.
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| Animal Protocol |
In vivo animal studies are not conducted with ferene disodium, as it is a colorimetric reagent rather than a therapeutic test article. The compound is not used in pharmacological or toxicological studies in animals. Its applications are limited to in vitro iron determination in biological and environmental samples. No in vivo efficacy or safety studies have been reported for this compound. The compound is not administered to animals.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of ferene disodium are not characterized as it is not a drug substance. Based on its physicochemical properties (molecular weight 454.34, high water solubility, polar nature), the compound would be expected to have very low oral bioavailability due to poor membrane permeability. It would likely be distributed primarily in extracellular fluid and rapidly cleared via renal excretion. The compound is not metabolized significantly. However, the compound is not intended for human exposure and has not been evaluated in formal pharmacokinetic studies.
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| Toxicity/Toxicokinetics |
Ferene disodium may cause skin and eye irritation. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves, safety glasses, and working in a fume hood. The compound should be stored in a cool, dry place away from light and moisture. No acute toxicity, mutagenicity, or carcinogenicity data are available. The compound is not intended for drug, household, or other uses. In case of contact, rinse with water and seek medical advice if irritation persists.
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| Additional Infomation |
Used to determine serum iron
Ferene disodium is a colorimetric reagent used for the sensitive and selective determination of iron (Fe²⁺) in biological and environmental samples. It is also known as ferene-S and 5,5'-[3-(2-pyridyl)-1,2,4-triazine-5,6-diyl]difuran-2-sulfonic acid disodium salt. The reagent forms a blue complex with Fe²⁺ with a high molar absorptivity at 593 nm. It is used in clinical chemistry for serum iron determination and in environmental monitoring. The compound has not undergone clinical trials and is not approved as a pharmaceutical. Its mechanism of action is chemical—forming a chromogenic complex with ferrous iron for quantification. |
| Molecular Formula |
C16H8N4NA2O8S2
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|---|---|
| Molecular Weight |
494.37
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| Exact Mass |
493.957
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| CAS # |
79551-14-7
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| PubChem CID |
133222
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.188 g/mL at 25 °C(lit.)
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| Boiling Point |
160-162 °C(lit.)
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| Melting Point |
46-49 °C(lit.)
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| Flash Point |
>230 °F
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| LogP |
3.423
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
32
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| Complexity |
787
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=NC(=C1)C2=NC(=C(N=N2)C3=CC=C(O3)S(=O)(=O)[O-])C4=CC=C(O4)S(=O)(=O)[O-].[Na+].[Na+]
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| InChi Key |
IVLSEFOVPQFJBB-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C16H10N4O8S2.2Na/c21-29(22,23)12-6-4-10(27-12)14-15(11-5-7-13(28-11)30(24,25)26)19-20-16(18-14)9-3-1-2-8-17-9;;/h1-8H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2
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| Chemical Name |
disodium;5-[3-pyridin-2-yl-6-(5-sulfonatofuran-2-yl)-1,2,4-triazin-5-yl]furan-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0228 mL | 10.1139 mL | 20.2278 mL | |
| 5 mM | 0.4046 mL | 2.0228 mL | 4.0456 mL | |
| 10 mM | 0.2023 mL | 1.0114 mL | 2.0228 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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