| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Pinolenic acid ethyl ester does not have a defined biological target as it is a fatty acid ester and research reagent rather than a pharmacologically active compound. Its function is biochemical—it serves as a lipid standard and as a model compound for studying fatty acid metabolism. Pinolenic acid itself has been shown to have appetite-suppressing effects and lipid-lowering properties, potentially through interactions with cholecystokinin (CCK) and other metabolic pathways. As an ethyl ester, it can be hydrolyzed to release pinolenic acid for biological studies. The compound itself is not evaluated for biological activity against specific targets.
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| ln Vitro |
In vitro, pinolenic acid ethyl ester exhibits no pharmacological activity as it is a fatty acid ester. Its utility is demonstrated in lipid analysis as a chromatographic standard for the identification and quantification of fatty acid esters by GC or LC-MS. The compound is also used in studies of lipid metabolism and fatty acid absorption. Pinolenic acid, the free fatty acid, has been shown to stimulate CCK secretion and suppress appetite, but the ethyl ester is primarily used as a research reagent. In cell-based assays, fatty acid esters can be taken up by cells and metabolized, but the ester itself is not tested for pharmacological activity.
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| ln Vivo |
Pinolenic acid ethyl ester is not a pharmacologically active agent and does not exhibit established in vivo therapeutic activity. It is used as a lipid standard and research reagent. The compound is not intended for human or animal exposure as a therapeutic agent. It is used in research laboratories for lipid analysis and biochemical studies. No therapeutic efficacy has been reported for this compound. The compound is not administered to animals in pharmacological studies as a test article. Its role is strictly analytical—serving as a standard for lipid analysis and as a precursor for pinolenic acid studies.
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| Enzyme Assay |
In vitro assays for pinolenic acid ethyl ester focus on its use as a lipid standard rather than receptor binding. A standard protocol involves preparing solutions of the compound in organic solvents (hexane, chloroform) and using it as a standard for GC or LC-MS analysis of fatty acid esters. The compound is also used as a substrate for lipase or esterase activity assays, where the enzyme hydrolyzes the ester bond, and the released ethanol or fatty acid is measured by enzymatic or colorimetric methods. Quality control includes GC purity analysis (>98%) and density measurement.
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| Cell Assay |
In vitro cell culture experiments with pinolenic acid ethyl ester typically involve studies of fatty acid metabolism or lipid uptake. Cells are cultured in appropriate media and treated with the fatty acid ester at concentrations ranging from 1-100 µM for 24-72 hours. Cellular lipid content is measured by lipid extraction followed by GC or LC-MS analysis. Esterase activity can be measured by monitoring the hydrolysis of the ester bond. Cell viability is assessed using MTT assays to ensure that observed effects are not due to cytotoxicity.
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| Animal Protocol |
In vivo animal studies with pinolenic acid ethyl ester are not well documented, as the compound is primarily used as a research reagent and lipid standard. If conducted, typical protocols for fatty acid ester administration would involve oral gavage in rodents at doses ranging from 10-100 mg/kg. Blood samples would be collected for pharmacokinetic analysis and lipid profiling. However, such studies are not standard for this compound as it is not a drug candidate. The compound is primarily used in analytical and biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of pinolenic acid ethyl ester are not well characterized as it is not a drug substance. As a fatty acid ester with a molecular weight of 306.48 and high lipophilicity (logP approximately 6-7), the compound would be expected to be absorbed through the gastrointestinal tract and metabolized by esterases to pinolenic acid and ethanol. The fatty acid would be further metabolized via β-oxidation and other lipid metabolic pathways. However, formal pharmacokinetic studies have not been conducted as the compound is not intended for human use.
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| Toxicity/Toxicokinetics |
Pinolenic acid ethyl ester is generally considered to have low toxicity as a fatty acid ester. Standard laboratory precautions should be followed when handling the compound, including the use of gloves and safety glasses. The compound should be stored in a cool, dry place away from light and moisture. No acute toxicity, mutagenicity, or carcinogenicity data are available. The compound is not intended for drug, household, or other uses. In case of contact, rinse with water and seek medical advice if irritation persists.
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| Additional Infomation |
Ethyl pinenoate is a lipid.
Pinolenic acid ethyl ester is a fatty acid ester used as a chromatographic standard for lipid analysis and in biochemical research. It is also known as ethyl pinolenate. Pinolenic acid, the free acid, is a polyunsaturated fatty acid found in pine nuts that has been studied for appetite suppression and lipid-lowering effects. The compound has not undergone clinical trials and is not approved as a pharmaceutical. Its mechanism of action is biochemical—serving as a standard and model compound for lipid analysis and fatty acid metabolism studies. |
| Molecular Formula |
C20H34O2
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|---|---|
| Molecular Weight |
306.48
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| Exact Mass |
306.255
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| CAS # |
493015-74-0
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| PubChem CID |
35027734
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| Appearance |
Colorless to light yellow liquid（Density：0.893±0.06 g/cm3）
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
390.6±31.0 °C at 760 mmHg
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| Flash Point |
100.8±23.2 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.475
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| LogP |
7.65
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
22
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| Complexity |
327
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCC/C=C\C/C=C\CC/C=C\CCCC(=O)OCC
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| InChi Key |
VNRWOSFYACMRHX-UCVSHGSNSA-N
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| InChi Code |
InChI=1S/C20H34O2/c1-3-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(21)22-4-2/h8-9,11-12,15-16H,3-7,10,13-14,17-19H2,1-2H3/b9-8-,12-11-,16-15-
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| Chemical Name |
ethyl (5Z,9Z,12Z)-octadeca-5,9,12-trienoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (326.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.16 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (8.16 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2629 mL | 16.3143 mL | 32.6286 mL | |
| 5 mM | 0.6526 mL | 3.2629 mL | 6.5257 mL | |
| 10 mM | 0.3263 mL | 1.6314 mL | 3.2629 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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