| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
L-Carnitine-d3 shares the same biological role as its non-deuterated form, L-carnitine. L-Carnitine is a quaternary ammonium compound involved in the transport of long-chain fatty acids into mitochondria for β-oxidation. It plays a critical role in energy metabolism. The labeled compound is used as an internal standard for the quantification of L-carnitine in biological samples.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The deuterated compound itself does not possess intrinsic pharmacological activity in vitro; its biological activity is identical to that of L-carnitine. L-Carnitine facilitates fatty acid transport into mitochondria and has been studied for its effects on energy metabolism and cardiovascular health. The labeled compound is used as an internal standard for quantifying L-carnitine in biological samples, not for evaluating its own pharmacological activity. |
| ln Vivo |
L-Carnitine-d3 is not used as a therapeutic agent; its non-deuterated form, L-carnitine, is a dietary supplement and has been studied for various clinical applications, including cardiovascular disease, peripheral neuropathy, and mitochondrial disorders. In vivo, L-carnitine is absorbed from the diet and synthesized in the liver and kidneys. The labeled compound is used in pharmacokinetic studies to accurately measure L-carnitine levels in plasma, urine, and tissue samples.
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| Enzyme Assay |
In vitro assays for L-carnitine-d3 focus on its use as an analytical standard. A standard protocol involves preparing a solution of the compound in an appropriate solvent (e.g., water or methanol) and using it as an internal standard for LC-MS or GC-MS analysis of L-carnitine in biological samples. The compound is added to samples before extraction and derivatization procedures. Quantification is performed by monitoring specific mass transitions for the labeled and unlabeled compounds.
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| Cell Assay |
In vitro cell culture experiments are not performed with L-carnitine-d3. When studying the effects of L-carnitine in cellular systems, the non-labeled compound is used. Cells are treated with L-carnitine, and fatty acid oxidation or energy metabolism is measured. The labeled compound is used as an internal standard for LC-MS analysis.
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| Animal Protocol |
In vivo animal studies are not conducted with L-carnitine-d3. When used in pharmacokinetic studies, the labeled compound serves as an internal standard for the quantification of L-carnitine in animal plasma or tissue samples.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-carnitine-d3 itself are not characterized, as it is not a drug substance. L-Carnitine is absorbed from the diet and synthesized endogenously. The labeled compound is used as an internal standard for pharmacokinetic studies.
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| Toxicity/Toxicokinetics |
L-Carnitine-d3 is not a therapeutic agent and has not been evaluated for toxicity in humans. L-Carnitine is a naturally occurring compound that is generally recognized as safe at recommended dietary levels. The labeled compound is for research use only and should be handled with standard laboratory precautions.
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| References | |
| Additional Infomation |
L-Carnitine-d3 hydrochloride is a stable isotope-labeled internal standard used for the quantification of L-carnitine in biological samples. It is also known as (R)-carnitine-d3 hydrochloride. The compound is used in analytical method development, quality control, and metabolic studies.
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| Molecular Formula |
C7H13D3CLNO3
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|---|---|
| Molecular Weight |
200.68
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| Exact Mass |
186.085
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| CAS # |
350818-62-1
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| Related CAS # |
(±)-Carnitine chloride;461-05-2
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| PubChem CID |
71309656
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| Appearance |
Off-white to light yellow solid powder
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| Melting Point |
131-135°C
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| LogP |
0.185
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
12
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| Complexity |
139
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H]C([2H])([2H])[N+](C)(C)C[C@@H](CC(=O)O)O.[Cl-]
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| InChi Key |
JXXCENBLGFBQJM-QKBJTWEASA-N
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| InChi Code |
InChI=1S/C7H15NO3.ClH/c1-8(2,3)5-6(9)4-7(10)11;/h6,9H,4-5H2,1-3H3;1H/t6-;/m1./s1/i1D3;
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| Chemical Name |
[(2R)-3-carboxy-2-hydroxypropyl]-dimethyl-(trideuteriomethyl)azanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1245.76 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (498.31 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9831 mL | 24.9153 mL | 49.8306 mL | |
| 5 mM | 0.9966 mL | 4.9831 mL | 9.9661 mL | |
| 10 mM | 0.4983 mL | 2.4915 mL | 4.9831 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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