| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary targets of 3'-methoxyapiin include reactive oxygen species (ROS) and inflammatory mediators. As a flavonoid, it acts as an antioxidant by scavenging free radicals and chelating metal ions. The compound also modulates inflammatory signaling pathways by inhibiting the production of pro-inflammatory cytokines and the activation of transcription factors such as NF-κB. Its effects on various enzymes, including kinases and phosphatases involved in cell signaling, have also been reported.
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| ln Vitro |
In vitro studies demonstrate that 3'-methoxyapiin exhibits significant antioxidant activity by scavenging DPPH and ABTS free radicals. The compound also shows anti-inflammatory activity by reducing the production of pro-inflammatory cytokines such as TNF-α and IL-6 in stimulated immune cells. It inhibits the activation of NF-κB and MAPK signaling pathways. Additionally, the compound shows protective effects against oxidative stress-induced cell damage and may have vasodilatory properties.
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| ln Vivo |
In vivo studies in animal models have shown that 3'-methoxyapiin exhibits antioxidant, anti-inflammatory, and cardiovascular benefits. In models of inflammation, the compound reduces the levels of inflammatory markers and tissue damage. In cardiovascular studies, it shows vasodilatory effects and may reduce blood pressure. The compound's presence in celery and other dietary sources suggests potential health benefits associated with the consumption of these foods.
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| Enzyme Assay |
Non-cellular assays for 3'-methoxyapiin typically involve measuring its antioxidant activity using DPPH, ABTS, or FRAP assays. The compound's ability to scavenge free radicals is quantified by measuring the reduction in absorbance at specific wavelengths. Enzyme inhibition assays may be used to assess the compound's effects on kinases or other enzymes. These cell-free systems allow for precise characterization of the compound's biochemical properties.
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| Cell Assay |
Cellular assays for 3'-methoxyapiin are conducted using various cell lines to assess its biological activities. Antioxidant activity is evaluated by measuring the compound's ability to reduce intracellular ROS levels in cells exposed to oxidative stress. Anti-inflammatory activity is assessed by measuring cytokine production and NF-κB activation in stimulated immune cells. Cell viability is measured using MTT assays to assess cytotoxicity. Vasodilatory effects may be studied using endothelial cell models.
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| Animal Protocol |
In vivo animal experiments for 3'-methoxyapiin typically use rodent models of inflammation, oxidative stress, or cardiovascular disease. The compound is administered orally or intraperitoneally, and its effects on inflammatory markers, oxidative stress parameters, and cardiovascular endpoints are assessed. Tissue samples are collected for histopathological examination and biochemical analysis. These studies provide insights into the compound's potential health benefits.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 3'-methoxyapiin have not been extensively characterized. As a glycoside with molecular weight approximately 594 g/mol, it may have limited oral bioavailability due to poor membrane permeability. The compound may be hydrolyzed by intestinal glycosidases to release the aglycone chrysoeriol. Its metabolism and elimination pathways have not been fully elucidated. Further pharmacokinetic studies are needed to support its development as a therapeutic agent.
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| Toxicity/Toxicokinetics |
Toxicological data for 3'-methoxyapiin are limited, as it is a natural product research compound. As a flavonoid glycoside found in edible plants, it is generally considered to have low toxicity. No significant toxicity has been reported in the available literature. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Other information includes the compound's presence in celery and other Apiaceae species, where it contributes to the health benefits associated with these foods. It is also known as chrysoeriol 7-O-apiosylglucoside, 3'-methoxyapioside, and graveobioside B. The compound is used as a reference standard in analytical chemistry for the quantification of flavonoids in plant extracts and food products. It is a valuable research tool for studying flavonoid biology and the health effects of dietary flavonoids.
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| Molecular Formula |
C27H30O15
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|---|---|
| Molecular Weight |
594.52
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| Exact Mass |
594.158
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| CAS # |
33579-63-4
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| PubChem CID |
74029660
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| Appearance |
Off-white to light yellow solid
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| Density |
1.72±0.1 g/cm3
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| Melting Point |
214-216 ºC
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| LogP |
-0.4
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
42
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| Complexity |
979
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[H]Oc1c([H])c([H])c(C2Oc3c(c(OC4(OC([H])(C(O[H])([H])[H])C([H])(O[H])C([H])(O[H])C4([H])OC4(OC([H])([H])C(O[H])(C(O[H])([H])[H])C4([H])O[H])[H])[H])c(c(O[H])c3C(=O)C=2[H])[H])[H])c([H])c1OC([H])([H])[H]
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| InChi Key |
GYQQQCVFOLKXGH-LDSFXQROSA-N
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| InChi Code |
InChI=1S/C27H30O15/c1-37-17-4-11(2-3-13(17)30)16-7-15(32)20-14(31)5-12(6-18(20)40-16)39-25-23(22(34)21(33)19(8-28)41-25)42-26-24(35)27(36,9-29)10-38-26/h2-7,19,21-26,28-31,33-36H,8-10H2,1H3/t19-,21-,22+,23-,24+,25-,26+,27-/m1/s1
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| Chemical Name |
7-[(2S,3R,4S,5S,6R)-3-[(2S,3R,4R)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-5-hydroxy-2-(4-hydroxy-3-methoxyphenyl)chromen-4-one
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| Synonyms |
3'-Methoxyapiin; Chrysoeriol 7-O-apiosylglucoside; Graveobioside B
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (168.20 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6820 mL | 8.4101 mL | 16.8203 mL | |
| 5 mM | 0.3364 mL | 1.6820 mL | 3.3641 mL | |
| 10 mM | 0.1682 mL | 0.8410 mL | 1.6820 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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