| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
The primary target of chromafenozide is the ecdysone receptor (EcR) in insects, a nuclear receptor that mediates the action of the molting hormone 20-hydroxyecdysone. As a non-steroidal ecdysone agonist, chromafenozide binds to the ecdysone receptor and activates the transcription of genes involved in molting and development. This leads to premature and lethal molting in susceptible insects.
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| ln Vitro |
In vitro studies demonstrate that chromafenozide activates ecdysone-responsive reporter gene assays in Sf9 and Kc cells. The compound acts as an ecdysone agonist, binding to the ecdysone receptor and initiating the molting process. Its activity is specific to lepidopteran insects, reflecting the selectivity of the ecdysone receptor in different insect orders.
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| ln Vivo |
In vivo studies show that chromafenozide effectively controls lepidopteran pests in agricultural settings. The insecticide is applied to crops and is absorbed by the target insects, where it induces premature molting and death. Its selective action, low toxicity to mammals, and minimal environmental persistence make it a valuable tool for integrated pest management.
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| Enzyme Assay |
Non-cellular assays for chromafenozide typically involve measuring its binding affinity to the ecdysone receptor using radioligand binding or surface plasmon resonance. The compound's ability to activate ecdysone-responsive gene transcription can be assessed using cell-free transcription assays. These cell-free systems allow for characterization of the compound's molecular interactions with its target receptor.
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| Cell Assay |
Cellular assays for chromafenozide are conducted using insect cell lines such as Sf9 (Spodoptera frugiperda) and Kc cells. Cells are transfected with ecdysone-responsive reporter genes and treated with the compound. Reporter activity is measured to assess the compound's ability to activate the ecdysone receptor. These assays are used to evaluate the insecticidal activity and mode of action of chromafenozide.
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| Animal Protocol |
In vivo animal experiments for chromafenozide are typically conducted in agricultural pest control studies. The compound is applied to crops infested with lepidopteran pests, and insect mortality, feeding damage, and crop yield are assessed. Toxicological studies in mammals are conducted to assess the compound's safety for non-target organisms and humans.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of chromafenozide have been studied in the context of its environmental fate and toxicology. The compound has a log P (octanol/water) of 2.7 at 22°C, a vapor pressure of ≤4 × 10-9 Pa at 25°C, and a solubility in water of 171 mg/L at 25°C. It is metabolized in insects and plants and has low persistence in the environment.
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| Toxicity/Toxicokinetics |
Toxicological data for chromafenozide indicate that it has low toxicity to mammals and non-target organisms. The compound has been evaluated by regulatory agencies and is approved for use in many countries. It is not classified as a carcinogen or developmental toxicant. Occupational exposure limits have been established to protect agricultural workers.
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| Additional Infomation |
Tryptamine hydrazine is a dihydrazide insecticide, and its function is similar to that of benzoyl hydrazine.
Other information includes chromafenozide's role as a dibenzoylhydrazine insecticide with hormonal and insecticidal activities specifically on lepidopteran insects. It is also known as cyclofenozide and ANS118. The compound is widely used in agricultural pest management due to its selective action, low toxicity to mammals, and minimal environmental persistence. In research, it serves as a tool to study insect endocrinology and hormone-regulated development. The compound is available as a research reagent and agricultural insecticide. |
| Molecular Formula |
C24H30N2O3
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|---|---|
| Molecular Weight |
394.51
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| Exact Mass |
394.226
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| CAS # |
143807-66-3
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| PubChem CID |
10157484
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| Appearance |
White to off-white solid powder
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| Density |
1.129g/cm3
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| Melting Point |
186.4°
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| Index of Refraction |
1.574
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| LogP |
4.913
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
29
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| Complexity |
589
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1=CC=C2C(CCCO2)=C1C)NN(C(C3=CC(C)=CC(C)=C3)=O)C(C)(C)C
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| InChi Key |
HPNSNYBUADCFDR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H30N2O3/c1-15-12-16(2)14-18(13-15)23(28)26(24(4,5)6)25-22(27)20-9-10-21-19(17(20)3)8-7-11-29-21/h9-10,12-14H,7-8,11H2,1-6H3,(H,25,27)
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| Chemical Name |
N'-tert-butyl-N'-(3,5-dimethylbenzoyl)-5-methyl-3,4-dihydro-2H-chromene-6-carbohydrazide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (633.70 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5348 mL | 12.6739 mL | 25.3479 mL | |
| 5 mM | 0.5070 mL | 2.5348 mL | 5.0696 mL | |
| 10 mM | 0.2535 mL | 1.2674 mL | 2.5348 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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