| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
PolyHEMA does not have a biological target in the traditional sense. It is a biocompatible biomaterial that interacts with biological tissues physically. Its high molecular weight enhances its mechanical stability and water retention, making it suitable for various biomedical applications.
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|---|---|
| ln Vitro |
Poly(2-hydroxyethyl methacrylate) (PolyHEMA) deters host cell absorption and breakdown while maintaining a moisture content akin to that of living tissue. It is simple to mold into many shapes and simple to sterilize[1]. When cells were grown on poly(2-hydroxyethyl methacrylate), their rates of proliferation were decreased; in hRPE cultures, poly (2-hydroxyethyl methacrylate) did not cause cell death. Numerous enormous spheroid colonies were produced by human retinal pigment epithelial (RPE) cells grown on poly (2-hydroxyethyl methacrylate). After megacolonies were recultured, poly(2-hydroxyethyl methacrylate) cell cultures were shown to include markers for retinal progenitor cells and hRPE cells [1].
In vitro, PolyHEMA hydrogels are characterized for their water content, mechanical properties, and biocompatibility. Cell culture studies are conducted to assess cell adhesion, proliferation, and differentiation on PolyHEMA surfaces. Its non-toxic and biocompatible properties are confirmed in these assays. |
| ln Vivo |
In vivo, PolyHEMA is used as a biomaterial in implantable devices and tissue engineering scaffolds. Its excellent water retention and biocompatibility make it suitable for contact lenses, soft tissue implants, and synthetic grafts. It is also used for the regeneration of nervous system tissues.
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| Enzyme Assay |
Non-cellular assays for PolyHEMA typically involve characterizing its physical and chemical properties. These include measurements of water content, swelling ratio, mechanical strength, and thermal stability. The material's purity and molecular weight are confirmed using techniques like gel permeation chromatography (GPC).
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| Cell Assay |
Cellular assays for PolyHEMA are conducted using various cell lines to assess its biocompatibility. Cells are cultured on PolyHEMA surfaces or in the presence of PolyHEMA extracts, and cell viability, adhesion, and proliferation are measured. These studies confirm the material's suitability for biomedical applications.
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| Animal Protocol |
In vivo animal experiments for PolyHEMA are conducted to evaluate its performance as an implantable biomaterial. The polymer is implanted subcutaneously or in other target tissues in animal models, and its biocompatibility, integration with host tissue, and biodegradation are assessed over time.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable for PolyHEMA, as it is a non-biodegradable, high-molecular-weight polymer used as a biomaterial, not a drug. It remains at the site of implantation and does not distribute systemically. Its high molecular weight prevents absorption.
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| Toxicity/Toxicokinetics |
Toxicological data for PolyHEMA indicate that it is non-toxic and biocompatible. It has been used safely in medical devices for decades. The polymer does not elicit significant inflammatory or immune responses. It is considered a safe material for its intended applications.
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| References |
[1]. Fatemeh Nazemroaya, et al. Induced Retro-Differentiation of Human Retinal Pigment Epithelial Cells on PolyHEMA. J Cell Biochem. 2017 Oct;118(10):3080-3089.
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| Additional Infomation |
biocompatible, hydrophilic, inert gel permeable to tissue fluid. It can be used as a microscope embedding agent, a coating for implants and prostheses, a coating for contact lenses, and for microspheres in adsorption studies.
See also: Poly(2-hydroxyethyl methacrylate) (note moved to). PolyHEMA is a cornerstone material in the field of biomaterials and biomedical engineering. It is used in the manufacture of contact lenses, drug delivery systems, biosensors, and tissue engineering scaffolds. Its high molecular weight (1,000,000) provides enhanced mechanical properties suitable for demanding applications. |
| Molecular Formula |
C6H10O3
|
|---|---|
| Molecular Weight |
1000000(Average)
|
| Exact Mass |
130.063
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| CAS # |
25249-16-5
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| PubChem CID |
18942519
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| Appearance |
White to off-white solid powder
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| Density |
1.054g/cm3
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| Boiling Point |
189ºC at 760mmHg
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| Flash Point |
97.2ºC
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| Vapour Pressure |
0.159mmHg at 25°C
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| Index of Refraction |
1.442
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| LogP |
0.098
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
11
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| Complexity |
129
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
OCCOC(C(=C)C)=O
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| InChi Key |
CRUFITZJZGASJI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H16O3/c1-4-8(2,3)7(10)11-6-5-9/h9H,4-6H2,1-3H3
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| Chemical Name |
2-hydroxyethyl 2,2-dimethylbutanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL
Ethanol: < 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of ethanol stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Suspended solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of ethanol stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of EtOH stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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