| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
As a detergent, n-Octyl β-D-glucopyranoside does not have a specific biological "target". Its function is physicochemical: it disrupts lipid-lipid and lipid-protein interactions in biological membranes, solubilizing membrane proteins by forming micelles around them. The compound interacts with the hydrophobic transmembrane domains of proteins and the lipid bilayer, effectively extracting the protein from its native membrane environment into a soluble, detergent-solubilized state.
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|---|---|
| ln Vitro |
In vitro, n-Octyl β-D-glucopyranoside is not evaluated for pharmacological activity but for its efficiency in solubilizing membrane proteins. Its performance is assessed by its ability to extract functional proteins from membranes, as measured by enzyme activity assays or binding studies, and by the stability of the solubilized proteins. It is a standard reagent for these purposes.
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| ln Vivo |
In vivo, n-Octyl β-D-glucopyranoside is not used as a therapeutic agent. Its application is entirely in vitro for laboratory research. It is not administered to living organisms for any biological effect.
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| Enzyme Assay |
A typical protocol for using n-Octyl β-D-glucopyranoside involves adding the detergent to a membrane preparation at a concentration above its CMC. The mixture is incubated with gentle agitation to allow solubilization, followed by centrifugation to remove insoluble material. The solubilized protein in the supernatant can then be used for further studies, such as purification, crystallization, or functional assays.
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| Cell Assay |
n-Octyl β-D-glucopyranoside is used in cell lysis buffers to solubilize membrane proteins for subsequent analysis. Cells are harvested, resuspended in a buffer containing the detergent, and lysed. The lysate is then centrifuged, and the supernatant containing the solubilized proteins is collected for downstream applications like immunoprecipitation, Western blot, or enzyme activity assays.
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| Animal Protocol |
n-Octyl β-D-glucopyranoside is not used in animal studies as an active compound. However, it can be used to solubilize proteins from animal tissues for ex vivo analysis.
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| ADME/Pharmacokinetics |
n-Octyl β-D-glucopyranoside has a molecular weight of 292.37 g/mol and a molecular formula of C14H28O6. It is a white, crystalline powder that is soluble in water and organic solvents. Its high CMC (0.7%) makes it easy to remove by dialysis. It is typically stored at room temperature, protected from moisture.
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| Toxicity/Toxicokinetics |
Toxicity Summary
The CIR expert panel concluded that the following 19 alkyl glucosides are safe for use in non-irritating formulations at their current methods of use and concentrations… Octyl glucoside… Safe for use in cosmetics, but subject to specific conditions. n-Octyl β-D-glucopyranoside is considered to have low toxicity and is commonly used in biochemical research. However, as a detergent, it can be irritating to skin, eyes, and mucous membranes. Standard laboratory safety precautions, including the use of gloves and safety goggles, should be followed when handling the compound. |
| References |
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| Additional Infomation |
Octyl β-D-glucopyranoside is a β-D-glucopyranoside in which the terminal hydrogen of β-D-glucopyranose is replaced by an octyl group. It is a plant metabolite. Octyl β-D-glucopyranoside has been reported in Rhodiola sachalinensis and Rhodiola rosea, and relevant data are available for reference.
n-Octyl β-D-glucopyranoside is a widely used, mild, non-ionic detergent for the solubilization and reconstitution of membrane proteins. Its ease of removal makes it particularly valuable for applications where the detergent needs to be eliminated, such as in protein crystallization and the reconstitution of membrane proteins into liposomes. It is a standard reagent in biochemistry and structural biology laboratories. It is not a drug and has no therapeutic applications. |
| Molecular Formula |
C14H28O6
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|---|---|
| Molecular Weight |
292.37
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| Exact Mass |
292.188
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| CAS # |
29836-26-8
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| PubChem CID |
62852
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
454.1±45.0 °C at 760 mmHg
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| Melting Point |
105 ℃
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| Flash Point |
228.4±28.7 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
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| Index of Refraction |
1.516
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| LogP |
1.03
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
20
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| Complexity |
250
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| Defined Atom Stereocenter Count |
5
|
| SMILES |
CCCCCCCCO[C@H]1[C@@H]([C@H]([C@@H]([C@H](O1)CO)O)O)O
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| InChi Key |
HEGSGKPQLMEBJL-RKQHYHRCSA-N
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| InChi Code |
InChI=1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
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| Chemical Name |
(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-octoxyoxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (342.03 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4203 mL | 17.1016 mL | 34.2032 mL | |
| 5 mM | 0.6841 mL | 3.4203 mL | 6.8406 mL | |
| 10 mM | 0.3420 mL | 1.7102 mL | 3.4203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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