| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
Alkyne-phenol targets proteins in proximity labeling assays. It is a probe for APEX2 proximity labeling, where APEX2 catalyzes the generation of a free radical on the phenolic oxygen, which then reacts with molecules within a 10-20 nm radius. This allows for the identification of proteins in close proximity to the APEX2-tagged protein of interest.
|
|---|---|
| ln Vitro |
Each probe was added to yeast cells expressing Su9-APEX2 at a final concentration of 2.5 mM for 30 minutes of incubation. The labeling reaction was then started by adding 1 mM H2O2. The most effective APEX2 substrate for yeast proteome labeling is alkyne-phenol [1]. At probe concentrations greater than 1 mM, APEX2-mediated Alkyne-phenol labeling in yeast mitochondria is more successful [1]. Compared to the conventional APEX2 substrate biotin-phenol (BP), alkyne-phenol offers greater coverage and an exceptionally high specificity (94%) for proteomic investigation of the mitochondrial matrix of intact yeast cells [1].
Alkyne-phenol is a probe for APEX2 proximity labeling. It is used to identify APEX labeling sites and elucidate the membrane topology of mitochondrial proteins. It significantly improves APEX labeling efficiency of intact yeast cells and has greater cell wall permeability than biotin-phenol. |
| ln Vivo |
Alkyne-phenol is used in APEX2 proximity labeling assays in vivo. It is used to identify APEX labeling sites and elucidate the membrane topology of mitochondrial proteins.
|
| Enzyme Assay |
The binding and labeling of Alkyne-phenol is mediated by the APEX2 enzyme. In a typical protocol, cells expressing an APEX2-fused protein are treated with alkyne-phenol and hydrogen peroxide. The APEX2 enzyme catalyzes the formation of a reactive species that labels nearby proteins. The labeled proteins are then conjugated to a fluorophore or affinity tag via click chemistry for detection and identification.
|
| Cell Assay |
Alkyne-phenol is used in cell-based assays for proximity labeling. Cells expressing an APEX2-fused protein are treated with alkyne-phenol and hydrogen peroxide. The labeled proteins are then detected by click chemistry with a fluorescent azide or by mass spectrometry after enrichment with an affinity tag.
|
| Animal Protocol |
Alkyne-phenol is used in animal studies for proximity labeling. Animals expressing an APEX2-fused protein are administered alkyne-phenol, and the labeled proteins are identified by mass spectrometry.
|
| ADME/Pharmacokinetics |
Alkyne-phenol has a molecular weight of 217.26 g/mol and a molecular formula of C13H15NO2. It is a solid that is soluble in DMSO. The compound is typically stored at -20°C.
|
| Toxicity/Toxicokinetics |
Toxicological data for Alkyne-phenol are not extensively detailed, as it is a research compound. It is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
|
| References | |
| Additional Infomation |
Alkyne-phenol is a research-grade compound used as a probe for APEX2 proximity labeling. Its alkyne group allows for the detection and enrichment of labeled proteins by click chemistry. It is a valuable tool for studying protein-protein interactions, subcellular localization, and membrane topology. The compound is not approved for therapeutic use and is strictly for laboratory research.
|
| Molecular Formula |
C13H15NO2
|
|---|---|
| Molecular Weight |
217.263703584671
|
| Exact Mass |
217.11
|
| CAS # |
1694495-59-4
|
| PubChem CID |
108021005
|
| Appearance |
White to off-white solid powder
|
| LogP |
1.7
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
16
|
| Complexity |
258
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C(CCC#C)NCCC1C=CC(=CC=1)O
|
| InChi Key |
CQZJBJSXQYZLEX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C13H15NO2/c1-2-3-4-13(16)14-10-9-11-5-7-12(15)8-6-11/h1,5-8,15H,3-4,9-10H2,(H,14,16)
|
| Chemical Name |
N-[2-(4-hydroxyphenyl)ethyl]pent-4-ynamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 50 mg/mL (230.14 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6028 mL | 23.0139 mL | 46.0278 mL | |
| 5 mM | 0.9206 mL | 4.6028 mL | 9.2056 mL | |
| 10 mM | 0.4603 mL | 2.3014 mL | 4.6028 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
