| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
TCEP hydrochloride does not have a specific biological target. It is a chemical reducing agent used in biochemical and molecular biology research. Its primary function is to reduce disulfide bonds in proteins and peptides, which is essential for protein preparation, thiol-based labeling, and bioconjugation.
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|---|---|
| ln Vitro |
The introduction of TCEP hydrochloride presents a potential substitute for the widely used DTT in vitro NF-κB-DNA binding processes. This can be achieved through the use of a 32P-labelled κB oligonucleotide and recombinant p50 protein. In binding experiments, DTT increases NF-κB-DNA binding at doses ranging from 0.25 to 2.6 mM. Nevertheless, only at Hg2+ concentrations higher than 100 μM can suppression of NF-κB binding occur in the presence of 0.25 mM DTT, and the outcomes vary greatly. On the other hand, at doses ranging from 0.25 to 6 mM, TCEP hydrochloride enhances NF-κB-DNA binding in a dose-dependent manner. Hg2+ inhibits NF-κB-DNA binding in binding assays at concentrations as low as 20 μM when even 6 mM TCEP hydrochloride is present[1]. Phosphate buffer is used to dissolve the human lactoferrin (hLF) peptide to a concentration of 0.1 mm. By adding a 30-fold molar excess of TCEP hydrochloride and then incubating for two hours at 37 °C, the disulfide linkages are reduced[2].
TCEP hydrochloride is a widely used reducing agent in biochemical and molecular biology research. Its in vitro activity is measured by its ability to reduce disulfide bonds in proteins and peptides, which can be assessed by Ellman's assay or by monitoring the loss of protein aggregation. |
| ln Vivo |
TCEP hydrochloride is not used as a therapeutic agent in vivo. It is a biochemical reagent used for protein preparation and thiol-based labeling reactions. Its utility is in vitro for the reduction of disulfide bonds in proteins and peptides.
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| Enzyme Assay |
The reducing activity of TCEP hydrochloride can be assessed using a standard disulfide reduction assay. A disulfide-containing compound, such as Ellman's reagent (DTNB), is incubated with TCEP, and the release of the thiol product is measured spectrophotometrically.
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| Cell Assay |
TCEP hydrochloride is used in cell lysis buffers for protein extraction to maintain proteins in a reduced state. It is also used in cell-based assays for thiol labeling and bioconjugation. The compound itself is not used to treat cells for a pharmacological effect.
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| Animal Protocol |
TCEP hydrochloride is not used in animal studies as an active compound. It is a reagent used in the preparation of protein samples from animal tissues. The compound is never administered to animals for therapeutic or investigative purposes.
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| ADME/Pharmacokinetics |
TCEP hydrochloride has a molecular weight of 286.65 g/mol and a molecular formula of C9H16ClO6P. It is a solid that is highly soluble in water (up to 310 g/L). The compound should be stored at room temperature.
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| Toxicity/Toxicokinetics |
Toxicological data for TCEP hydrochloride are not extensively detailed, as it is a research compound. It is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
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| References |
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| Additional Infomation |
TCEP hydrochloride is a research-grade compound used as a powerful and stable reducing agent for the cleavage of disulfide bonds in proteins and peptides. Its odorless nature and stability make it a preferred alternative to DTT and 2-mercaptoethanol. It is widely used in protein biochemistry, bioconjugation, and molecular biology. The compound is not approved for therapeutic use and is strictly for laboratory research.
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| Molecular Formula |
C9H16CLO6P
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|---|---|
| Molecular Weight |
286.65
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| Exact Mass |
286.037
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| CAS # |
51805-45-9
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| Related CAS # |
TCEP-d16 hydrochloride;1174025-33-2
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| PubChem CID |
2734570
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| Appearance |
White to light yellow solid powder
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| Density |
1.041 g/mL at 25 °C
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| Boiling Point |
519.4ºC at 760 mmHg
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| Melting Point |
177 °C
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| Flash Point |
267.9ºC
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| Index of Refraction |
n20/D 1.367
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| LogP |
1.694
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
17
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| Complexity |
222
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CP(CCC(=O)O)CCC(=O)O)C(=O)O.Cl
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| InChi Key |
PBVAJRFEEOIAGW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
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| Chemical Name |
3-[bis(2-carboxyethyl)phosphanyl]propanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (348.86 mM)
H2O: 50 mg/mL (174.43 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (348.86 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4886 mL | 17.4429 mL | 34.8857 mL | |
| 5 mM | 0.6977 mL | 3.4886 mL | 6.9771 mL | |
| 10 mM | 0.3489 mL | 1.7443 mL | 3.4886 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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