| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Cholesterol-d6-1 targets the same biological structures as cholesterol. Cholesterol is a key component of cellular membranes, comprising 20-25% of their structural components and influencing membrane fluidity and permeability characteristics. As a deuterated compound, its primary use is as a tracer for studying cholesterol metabolism and membrane biology.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Cholesterol-d6-1 itself is not evaluated for traditional in vitro biological activity. Its "activity" is as a stable isotope-labeled tracer for studying cholesterol metabolism and membrane biology. It is used to track the fate of cholesterol in cells and biological systems. No pharmacological in vitro activity is associated with this compound. |
| ln Vivo |
Cholesterol-d6-1 is used as a tracer to study cholesterol metabolism in vivo. It can be administered to animals to track the absorption, distribution, metabolism, and excretion of cholesterol. The compound is used in research to understand the role of cholesterol in health and disease.
|
| Enzyme Assay |
Cholesterol-d6-1 is used as a tracer in metabolic studies. Its performance is assessed by its isotopic purity and its ability to provide accurate and precise tracking of cholesterol in biological systems. The compound is typically analyzed by GC-MS or LC-MS.
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| Cell Assay |
Cholesterol-d6-1 is used in cell culture to study cholesterol metabolism. Cells are treated with the labeled compound, and its incorporation into cellular membranes and metabolites is analyzed. The compound allows for the precise tracking of cholesterol in cellular systems.
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| Animal Protocol |
Cholesterol-d6-1 is used in animal studies to study cholesterol metabolism. The compound is administered to animals, and its distribution and metabolism are tracked in various tissues. This allows researchers to study the role of cholesterol in health and disease.
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| ADME/Pharmacokinetics |
Cholesterol-d6-1 has a molecular weight of 392.69 g/mol and a molecular formula of C27H40D6O. It is a deuterium-labeled form of cholesterol. Cholesterol is the major sterol in mammals, making up 20-25% of the structural component of the plasma membrane. The compound is typically stored at -20°C.
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| Toxicity/Toxicokinetics |
Toxicological data for Cholesterol-d6-1 are not extensively detailed, as it is a research compound. It is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
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| References | |
| Additional Infomation |
Cholesterol-d6-1 is a deuterium-labeled form of cholesterol, the major sterol in mammals. Cholesterol makes up 20-25% of the structural component of the plasma membrane. It is used as a stable isotope for studying cholesterol metabolism and membrane biology. It is not approved for therapeutic use.
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| Molecular Formula |
C27H40D6O
|
|---|---|
| Molecular Weight |
392.69
|
| Exact Mass |
392.393
|
| CAS # |
92543-08-3
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| Related CAS # |
Cholesterol;57-88-5
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| PubChem CID |
16213400
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| Appearance |
White to off-white solid powder
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| Density |
1.088 g/mL at 25ºC
|
| Boiling Point |
360ºC(lit.)
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| Melting Point |
147-149ºC(lit.)
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| Index of Refraction |
1.525
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| LogP |
7.388
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
|
| Heavy Atom Count |
28
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| Complexity |
591
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
[2H]C1=C2[C@](CC([C@](C2([2H])[2H])([2H])O)([2H])[2H])([C@H]3CC[C@]4([C@H]([C@@H]3C1)CC[C@@H]4[C@H](C)CCCC(C)C)C)C
|
| InChi Key |
HVYWMOMLDIMFJA-HHPLMCQASA-N
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| InChi Code |
InChI=1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1/i9D,13D2,17D2,21D
|
| Chemical Name |
(3S,8S,9S,10R,13R,14S,17R)-2,2,3,4,4,6-hexadeuterio-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-1,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol: 10 mg/mL (25.47 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (2.55 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear EtOH stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (2.55 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear EtOH stock solution to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5465 mL | 12.7327 mL | 25.4654 mL | |
| 5 mM | 0.5093 mL | 2.5465 mL | 5.0931 mL | |
| 10 mM | 0.2547 mL | 1.2733 mL | 2.5465 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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