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| Other Sizes |
| Targets |
3-Methylcarbazole targets various biological pathways through its carbazole core. Carbazole derivatives are known to interact with enzymes, receptors, and DNA. The compound's antimicrobial activity suggests interactions with microbial cell components. Its anticancer properties suggest interactions with pathways regulating cell proliferation and apoptosis. Its anti-inflammatory activity may involve modulation of inflammatory cytokine production. Further research is needed to identify its specific molecular targets.
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| ln Vitro |
In vitro studies have demonstrated that 3-Methylcarbazole exhibits potential biological activities characteristic of carbazole derivatives. Carbazoles are known for their antimicrobial, anticancer, and anti-inflammatory activities. The compound's effects on cell proliferation and viability have been evaluated in various cell-based systems. These in vitro findings support its potential applications in carbazole research and drug discovery.
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| ln Vivo |
In vivo studies of 3-Methylcarbazole are limited as the compound is primarily used as a research chemical. Carbazole derivatives have been studied in animal models for their antimicrobial, anticancer, and anti-inflammatory activities. However, comprehensive in vivo studies specifically targeting 3-Methylcarbazole are not well documented. The compound is intended for research use only.
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| Enzyme Assay |
In vitro enzyme assays for 3-Methylcarbazole typically involve testing its activity against targets relevant to carbazole derivatives. Enzyme inhibition assays are performed for targets such as kinases, topoisomerases, and cytochrome P450 enzymes. Antimicrobial activity is assessed using standard disc diffusion or broth microdilution methods. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays for 3-Methylcarbazole involve culturing cancer cell lines to evaluate its potential anticancer activity. Cells are treated with varying concentrations of the compound and cell viability is assessed using MTT or similar colorimetric assays. Apoptosis is quantified using flow cytometry with Annexin V/PI staining. For antimicrobial studies, bacterial or fungal cultures are treated and cell viability is monitored. All experiments are performed in triplicate with appropriate controls.
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| Animal Protocol |
In vivo animal experiments for 3-Methylcarbazole would be conducted to evaluate its antimicrobial, anticancer, and anti-inflammatory activities. For antimicrobial studies, infected animals would be treated and pathogen load assessed. For anticancer studies, tumor-bearing animals would be treated and tumor growth monitored. For anti-inflammatory studies, animals with induced inflammation would be treated and inflammatory markers measured. Parameters assessed would include body weight, organ weights, and histopathology. Control groups receiving vehicle alone would be included for comparison. All procedures would comply with institutional animal care and use committee guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of 3-Methylcarbazole reflect its nature as a small carbazole derivative. It has a molecular formula of C13H11N. As a lipophilic heterocyclic compound, it would be absorbed through the gastrointestinal tract and distributed throughout the body. The compound is expected to be metabolized through standard xenobiotic pathways in the liver. Complete pharmacokinetic profiling would require further systematic studies.
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| Toxicity/Toxicokinetics |
The toxicity profile of 3-Methylcarbazole has been evaluated in the context of its use as a research chemical. As a carbazole derivative, it may have biological activities that should be carefully evaluated. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile.
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| References | |
| Additional Infomation |
According to reports, 3-methyl-9H-carbazole has been found in Virginia creeper, Murraya paniculata, and other organisms with available data.
3-Methylcarbazole (NSC 10154, CAS# 4630-20-0) is a carbazole derivative with the molecular formula C13H11N. It is a nitrogen-containing heterocyclic compound featuring a carbazole core with a methyl group at the 3 position. Carbazole derivatives are known for various biological activities including antimicrobial, anticancer, and anti-inflammatory properties. 3-Methylcarbazole is used in research applications for studying carbazole chemistry and pharmacology. It is intended for research use only. |
| Molecular Formula |
C13H11N
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|---|---|
| Molecular Weight |
181.23
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| Exact Mass |
181.089
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| CAS # |
4630-20-0
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| PubChem CID |
20746
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| Appearance |
White to light brown solid
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
369.1±11.0 °C at 760 mmHg
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| Melting Point |
208 °C
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| Flash Point |
165.4±11.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.737
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| LogP |
4.18
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
14
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| Complexity |
213
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1=CC2=C(C=C1)NC3=CC=CC=C23
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| InChi Key |
PHKYYUQQYARDIU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H11N/c1-9-6-7-13-11(8-9)10-4-2-3-5-12(10)14-13/h2-8,14H,1H3
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| Chemical Name |
3-methyl-9H-carbazole
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| Synonyms |
NSC 10154; NSC 10154; NSC10154; 3-Methylcarbazole
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (551.79 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (13.79 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5179 mL | 27.5893 mL | 55.1785 mL | |
| 5 mM | 1.1036 mL | 5.5179 mL | 11.0357 mL | |
| 10 mM | 0.5518 mL | 2.7589 mL | 5.5179 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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