| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Tetramethylthiuram monosulfide targets sulfur-containing enzymes and proteins through its thiuram structure. As a sulfur-containing compound, it can interact with thiol groups in proteins and enzymes. Its use as a vulcanization accelerator involves the formation of sulfur crosslinks in rubber. Its fungicidal activity suggests interactions with fungal cellular components. Further research is needed to identify its specific molecular targets in biological systems.
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| ln Vitro |
In vitro studies of Tetramethylthiuram monosulfide have focused on its role as a vulcanization accelerator and fungicide. The compound's chemical properties and reactivity have been characterized. Its effects on rubber vulcanization have been studied. These in vitro studies provide foundational data for understanding the compound's properties and its applications in rubber chemistry and fungicide research.
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| ln Vivo |
In vivo studies of Tetramethylthiuram monosulfide are limited as the compound is primarily used in industrial applications rather than as a therapeutic agent. As a fungicide, it may have effects on fungal pathogens in vivo. However, comprehensive in vivo pharmacological studies specifically targeting Tetramethylthiuram monosulfide are not well documented. The compound is intended for research use only.
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| Enzyme Assay |
In vitro enzyme assays for Tetramethylthiuram monosulfide typically involve testing its interactions with thiol-containing enzymes. Enzyme activity is measured by monitoring substrate conversion in the presence of varying concentrations of the compound. The compound's purity and identity are assessed using analytical chemistry methods. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays for Tetramethylthiuram monosulfide involve culturing fungal cells to evaluate its fungicidal activity. Fungal cells are treated with varying concentrations of the compound and cell viability is monitored. Cell viability is assessed using standard assays. All experiments are performed in triplicate with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for Tetramethylthiuram monosulfide are conducted for toxicology and safety assessment. Animals are administered the compound and observed for signs of toxicity. Parameters assessed include body weight, organ weights, and histopathology. Control groups receiving vehicle alone are included for comparison. All procedures comply with institutional animal care and use committee guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Tetramethylthiuram monosulfide reflect its nature as a small sulfur-containing compound. It has a molecular formula of C6H12N2S3. As a lipophilic compound, it would be absorbed through the gastrointestinal tract and distributed throughout the body. The compound is metabolized through standard xenobiotic pathways. Complete pharmacokinetic profiling would require further systematic studies.
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| Toxicity/Toxicokinetics |
Interactions
In adult female Wistar rats, pre-gavage administration of 16 or 256 μM/kg cyanamide, tetramethylthiuram disulfide (TMTD), tetramethylthiuram monosulfide (TMTM), zinc fumarate, or zineb, followed by intraperitoneal injection of 2 g/kg ethanol 90 minutes or 18 hours later, resulted in a significant increase in serum acetaldehyde levels within 90–240 minutes after ethanol administration (but this was not observed after 90 minutes of zineb treatment or 18 hours of low-dose cyanamide treatment). Pre-administration of ANTU or ANIT at doses within the LD50 range followed by the same time period did not affect serum acetaldehyde levels in rats receiving the same dose of ethanol. Oral administration of 16 μM/kg TMTM and TMTD resulted in an increase in serum acetaldehyde levels that could last up to 48 hours. The effects of cyanamide began to appear after 45 minutes. The toxicity profile of Tetramethylthiuram monosulfide has been evaluated in the context of its industrial use. As a sulfur-containing compound, it may have irritant and toxic properties. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile. |
| References |
[1]. Coleman, et al. Vibrational spectra of thiuram sulfides. Normal coordinate analysis of tetramethylthiuram mono-, di-, and tetrasulfides. Journal of Polymer Science, Polymer Physics Edition.Volume: 12.Issue: 5.Pages: 1001-13.Journal.1974.
[2]. Yingtao Sun, et al. Synergistic Effect of Copper Sulfate and Tetramethylthiuram Monosulfide Induced Metal-Coordination Cross-Linking in Nitrile–Butadiene Rubber. 25 April 2021. |
| Additional Infomation |
Tetramethylthiuram monosulfide is a standardized chemical allergen. Its physiological effects are achieved through increased histamine release and cell-mediated immune responses.
Tetramethylthiuram monosulfide (TMTM, CAS# 97-74-5) is a sulfur-containing organic compound used as a vulcanization accelerator in the rubber industry. It has the molecular formula C6H12N2S3. TMTM is also used as a fungicide and in research applications for studying sulfur chemistry and rubber vulcanization. The compound is a member of the thiuram disulfide family. It is intended for research use only. |
| Molecular Formula |
C6H12N2S3
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|---|---|
| Molecular Weight |
208.37
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| Exact Mass |
208.016
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| CAS # |
97-74-5
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| PubChem CID |
7347
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| Appearance |
Yellow powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
260.9±23.0 °C at 760 mmHg
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| Melting Point |
109.5 °C
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| Flash Point |
111.6±22.6 °C
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| Vapour Pressure |
0.0±0.5 mmHg at 25°C
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| Index of Refraction |
1.641
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| LogP |
0.87
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
11
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| Complexity |
147
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S=C(N(C)C)SC(N(C)C)=S
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| InChi Key |
REQPQFUJGGOFQL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H12N2S3/c1-7(2)5(9)11-6(10)8(3)4/h1-4H3
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| Chemical Name |
dimethylcarbamothioyl N,N-dimethylcarbamodithioate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (1199.79 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (9.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (9.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7992 mL | 23.9958 mL | 47.9916 mL | |
| 5 mM | 0.9598 mL | 4.7992 mL | 9.5983 mL | |
| 10 mM | 0.4799 mL | 2.3996 mL | 4.7992 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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