| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Sophorabioside targets inflammatory pathways and oxidative stress. As a flavonoid glycoside, it modulates oxidative stress and inhibits inflammatory mediators. The compound's mechanism involves the modulation of inflammatory signaling pathways and reduction of oxidative damage. Its flavonoid structure allows for interactions with various enzymes and signaling pathways involved in inflammation. Further research is needed to identify its specific molecular targets and mechanisms of action.
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| ln Vitro |
In vitro studies have demonstrated that Sophorabioside modulates oxidative stress and inhibits inflammatory mediators. As a flavonoid glycoside from Sophora japonica, its anti-inflammatory and antioxidant effects have been characterized. The compound has a purity of ≥98%. These in vitro findings support its potential applications in inflammation and oxidative stress research.
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| ln Vivo |
In vivo studies of Sophorabioside have been conducted in the context of anti-osteoporotic compound isolation from Sophora japonica seeds. The compound's anti-inflammatory and antioxidant properties suggest potential for in vivo evaluation in models of inflammatory diseases and oxidative stress. However, comprehensive in vivo pharmacological studies specifically targeting Sophorabioside are not well documented. The compound is intended for research use only.
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| Enzyme Assay |
In vitro enzyme inhibition assays for Sophorabioside typically involve testing its anti-inflammatory and antioxidant activities. Anti-inflammatory activity is assessed by measuring inhibition of inflammatory mediator production. Antioxidant activity is assessed using cell-free systems such as DPPH radical scavenging assays. The compound's purity (≥98%) and identity are confirmed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry.
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| Cell Assay |
In vitro cell-based assays for Sophorabioside involve culturing immune cells to evaluate its anti-inflammatory and antioxidant effects. Cells are treated with varying concentrations of the compound and stimulated with inflammatory stimuli. Pro-inflammatory cytokine production is measured by ELISA. Oxidative stress markers are measured using fluorescent probes. Cell viability is assessed using MTT or similar colorimetric assays. All experiments are performed with appropriate controls.
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| Animal Protocol |
In vivo animal experiments for Sophorabioside would be conducted to evaluate its anti-inflammatory and antioxidant activities. For anti-osteoporotic studies, animal models of osteoporosis would be used. For anti-inflammatory studies, animals with induced inflammation would be treated and inflammatory markers measured. Parameters assessed would include bone density, inflammatory markers, and histopathology. Control groups receiving vehicle alone would be included for comparison. All procedures would comply with institutional animal care and use committee guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Sophorabioside reflect its nature as a flavonoid glycoside. It has a molecular weight of 578.52 and the molecular formula C27H30O14. The compound is freely soluble in pyridine and soluble in hot alcohol and hot acetone. It is slightly soluble in boiling water. The anhydrous substance has a melting point of 248°C. Complete pharmacokinetic profiling would require further systematic studies.
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| Toxicity/Toxicokinetics |
The toxicity profile of Sophorabioside has been evaluated in the context of its use as a research chemical. As a naturally occurring flavonoid glycoside from Sophora japonica, it is expected to have a favorable safety profile at research concentrations. The compound has a purity of ≥98%. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| References | |
| Additional Infomation |
Reports have indicated that Japanese Sophora flavescens contains matrine, and relevant data is available for reference.
Sophorabioside (CAS# 2945-88-2) has the molecular formula C27H30O14 and a molecular weight of 578.52. The compound is a flavonoid glycoside isolated from Sophora japonica. Sophorabioside modulates oxidative stress and inhibits inflammatory mediators. The compound has a purity of ≥98% and melts at 248°C (slight decomposition). It is freely soluble in pyridine and soluble in hot alcohol and hot acetone. |
| Molecular Formula |
C27H30O14
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|---|---|
| Molecular Weight |
578.52
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| Exact Mass |
578.164
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| CAS # |
2945-88-2
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| PubChem CID |
11968944
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| Appearance |
White to light yellow solid
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| Melting Point |
248℃
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| LogP |
-0.2
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
41
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| Complexity |
939
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| Defined Atom Stereocenter Count |
10
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| SMILES |
O([C@@]1([H])[C@@]([H])([C@@]([H])([C@]([H])([C@]([H])(C([H])([H])[H])O1)O[H])O[H])O[H])[C@@]1([H])[C@]([H])(OC2C([H])=C([H])C(C3=C([H])OC4=C([H])C(=C([H])C(=C4C3=O)O[H])O[H])=C([H])C=2[H])O[C@]([H])(C([H])([H])O[H])[C@]([H])([C@]1([H])O[H])O[H]
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| InChi Key |
SNJVNAXLTOIYQN-XQCQZFFBSA-N
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| InChi Code |
InChI=1S/C27H30O14/c1-10-19(31)22(34)24(36)26(38-10)41-25-23(35)21(33)17(8-28)40-27(25)39-13-4-2-11(3-5-13)14-9-37-16-7-12(29)6-15(30)18(16)20(14)32/h2-7,9-10,17,19,21-31,33-36H,8H2,1H3/t10-,17+,19-,21+,22+,23-,24+,25+,26-,27+/m0/s1
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| Chemical Name |
3-[4-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxyphenyl]-5,7-dihydroxychromen-4-one
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| Synonyms |
Sophorabioside
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (86.43 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (2.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL | |
| 5 mM | 0.3457 mL | 1.7285 mL | 3.4571 mL | |
| 10 mM | 0.1729 mL | 0.8643 mL | 1.7285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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