| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
|
||
| Other Sizes |
| Targets |
Mefenpyr-diethyl targets herbicide detoxification pathways in plants. As a safener, it enhances the metabolization and detoxification of herbicides in treated plants. The compound activates herbicide-detoxifying enzymes such as cytochrome P450 monooxygenases and glutathione S-transferases, which accelerate the breakdown of herbicides. Its targets are plant metabolic enzymes rather than mammalian biological targets. The compound is used in herbicidal and herbicide-safener combinations.
|
|---|---|
| ln Vitro |
In vitro studies of Mefenpyr-diethyl have focused on its role as a herbicide safener. The compound's ability to enhance herbicide detoxification has been characterized in plant systems. It is used in herbicidal and herbicide-safener combinations as a resistant breaking solution for modern agriculture. These in vitro studies provide foundational data for understanding the compound's utility in agricultural weed management.
|
| ln Vivo |
In vivo studies of Mefenpyr-diethyl have demonstrated its efficacy as a herbicide safener in cereal crops. After absorption, the metabolization and detoxification of herbicides occur in treated plants. The compound is used in combination with certain herbicides to improve crop tolerance. It is not an active herbicide substance and is intended for agricultural use only.
|
| Enzyme Assay |
In vitro enzyme assays for Mefenpyr-diethyl typically involve testing its effects on herbicide-detoxifying enzymes in plant systems. Enzyme activity is measured by monitoring the metabolism of herbicides in the presence of varying concentrations of the compound. The compound's purity (typically ≥98%) and identity are confirmed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry.
|
| Cell Assay |
In vitro cell-based assays for Mefenpyr-diethyl involve culturing plant cells to evaluate its effects on herbicide detoxification. Plant cells are treated with varying concentrations of the compound and herbicide metabolism is assessed. The compound's effects on detoxifying enzyme activities are measured. All experiments are performed with appropriate controls to ensure statistical reliability.
|
| Animal Protocol |
In vivo plant experiments for Mefenpyr-diethyl are conducted to evaluate its safener efficacy. Plants are treated with the compound in combination with herbicides and crop tolerance is assessed. Parameters assessed include crop injury, herbicide efficacy, and yield. The compound's ability to enhance herbicide detoxification is evaluated. Control groups receiving herbicides without the safener are included for comparison.
|
| ADME/Pharmacokinetics |
The pharmacokinetic properties of Mefenpyr-diethyl reflect its nature as a herbicide safener. It has a molecular weight of 373.23 and the molecular formula C16H18Cl2N2O4. The compound appears as a white-yellow solid. As a small lipophilic molecule, it is absorbed through plant foliage and distributed throughout the plant. Complete pharmacokinetic profiling in plants would require further systematic studies.
|
| Toxicity/Toxicokinetics |
The toxicity profile of Mefenpyr-diethyl has been evaluated for environmental and occupational safety. As an agricultural chemical, it may have toxic effects on non-target organisms. Proper handling procedures including use of personal protective equipment are recommended when working with the compound. The compound is not approved for human therapeutic use and is intended for agricultural research purposes only.
|
| References | |
| Additional Infomation |
Mefenpyr-diethyl (CAS# 135590-91-9) is also known as HOE-107892. It has the molecular formula C16H18Cl2N2O4 and a molecular weight of 373.23. The compound is a foliar-acting safener of the pyrazoline chemical group. It is used as a safener in combination with herbicides to enhance herbicide detoxification in treated plants. Mefenpyr-diethyl is used as a resistant breaking solution for modern agriculture.
|
| Molecular Formula |
C16H18CL2N2O4
|
|---|---|
| Molecular Weight |
373.23
|
| Exact Mass |
372.064
|
| CAS # |
135590-91-9
|
| PubChem CID |
10937610
|
| Appearance |
Off-white to light yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
451.1±55.0 °C at 760 mmHg
|
| Melting Point |
52 °C
|
| Flash Point |
226.6±31.5 °C
|
| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
| Index of Refraction |
1.576
|
| LogP |
3.2
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
24
|
| Complexity |
528
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCOC(=O)C1=NN(C(C1)(C)C(=O)OCC)C2=C(C=C(C=C2)Cl)Cl
|
| InChi Key |
OPGCOAPTHCZZIW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H18Cl2N2O4/c1-4-23-14(21)12-9-16(3,15(22)24-5-2)20(19-12)13-7-6-10(17)8-11(13)18/h6-8H,4-5,9H2,1-3H3
|
| Chemical Name |
diethyl 1-(2,4-dichlorophenyl)-5-methyl-4H-pyrazole-3,5-dicarboxylate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (267.93 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.70 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6793 mL | 13.3966 mL | 26.7931 mL | |
| 5 mM | 0.5359 mL | 2.6793 mL | 5.3586 mL | |
| 10 mM | 0.2679 mL | 1.3397 mL | 2.6793 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
