| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Biorobin (Kaempferol 3-O-robinobioside) modulates lymphocyte proliferation, though specific molecular targets (e.g., receptors/enzymes) and binding affinities (IC50/Ki/EC50) were not characterized in the study.
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|---|---|
| ln Vitro |
Biorobin (kaempferol 3-O-robinobioside) significantly inhibited concanavalin A-induced lymphocyte proliferation in mouse splenocytes at 100 μg/mL, showing 50.5% ± 1.5% suppression compared to controls.
Exhibited dose-dependent immunomodulatory effects, with lower inhibition (25.2% ± 1.8%) at 50 μg/mL. Demonstrated stronger immunosuppressive activity than its aglycone kaempferol at equivalent concentrations. [1] Biorobin, also known as Kaempferol 3-O-robinobioside, inhibits human T-cell proliferation in vitro (IC50=25 mg/mL)[1]. |
| Cell Assay |
Lymphocyte proliferation assay: Mouse splenocytes (5×10⁵ cells/well) cultured with concanavalin A (5 μg/mL) ± Biorobin (0-100 μg/mL) in RPMI-1640 medium supplemented with 10% FBS.
After 72-hour incubation, cell proliferation measured by MTT assay: Addition of MTT reagent (5 mg/mL) followed by 4-hour incubation and formazan crystal dissolution with SDS-HCl. Absorbance read at 570 nm, with results expressed as percentage inhibition relative to mitogen-stimulated controls. [1] |
| References | |
| Additional Infomation |
Biorobin is a flavonol compound isolated from Brazilian lotus (Amaranthaceae). Its glycosyl moiety (robinobiose: rhamnose-α(1→6)-galactose) has stronger immunosuppressive activity than the aglycone kaempferol. It represents a novel immunomodulator from a Brazilian medicinal plant. [1] Biorobin has also been reported in tea (Camellia sinensis), soybean (Glycine max), and other organisms with relevant data.
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| Molecular Formula |
C27H30O15
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|---|---|
| Molecular Weight |
594.52
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| Exact Mass |
594.158
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| CAS # |
17297-56-2
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| PubChem CID |
15944778
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| Appearance |
Light yellow to yellow solid
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
941.7±65.0 °C at 760 mmHg
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| Melting Point |
221-223℃
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| Flash Point |
312.8±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.744
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| Source |
Originated from plants: Euphorbiaceae Acaly phaindica L.
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| LogP |
1.96
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| Hydrogen Bond Donor Count |
9
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
42
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| Complexity |
985
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| Defined Atom Stereocenter Count |
10
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| SMILES |
CC1OC(OCC2OC(OC3C(=O)C4C(=CC(O)=CC=4O)OC=3C3=CC=C(O)C=C3)C(O)C(O)C2O)C(O)C(O)C1O
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| InChi Key |
RTATXGUCZHCSNG-KYGWAIEOSA-N
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| InChi Code |
InChI=1S/C27H30O15/c1-9-17(31)20(34)22(36)26(39-9)38-8-15-18(32)21(35)23(37)27(41-15)42-25-19(33)16-13(30)6-12(29)7-14(16)40-24(25)10-2-4-11(28)5-3-10/h2-7,9,15,17-18,20-23,26-32,34-37H,8H2,1H3/t9-,15+,17-,18-,20+,21-,22+,23+,26+,27-/m0/s1
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| Chemical Name |
5,7-dihydroxy-2-(4-hydroxyphenyl)-3-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one
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| Synonyms |
Kaempferol 3-O-robinobioside; 5,7-Dihydroxy-2-(4-hydroxyphenyl)-3-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one; Kaempferol-3-O-robinobioside; Kaempferol 3-O-robinoside; MLS000574999; CHEMBL79790; Biorobin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~168.2 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6820 mL | 8.4101 mL | 16.8203 mL | |
| 5 mM | 0.3364 mL | 1.6820 mL | 3.3641 mL | |
| 10 mM | 0.1682 mL | 0.8410 mL | 1.6820 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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