| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Glycine receptors; GABA receptors; opioid receptors. Humantenmine (Gelsenicine) is a Gelsemium alkaloid that may interact with glycine receptors, GABA receptors, or opioid receptors. Gelsemium alkaloids are known for their potent neurotoxic effects and analgesic activities. The compound's mechanism of action may involve modulation of inhibitory neurotransmission or opioid receptor signaling. However, the exact mechanism of action of humantenmine is not fully characterized.
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| ln Vitro |
The mice that receive intragastrically treated humantenmine (HMT) have a reduced 14-day survival rate of 17%, along with hepatic injury and elevated levels of alanine aminotransferase (ALT)/aspartate aminotransferase (AST). The metabolism and elimination of HMT are mediated by CYP3A4/5[1][2].
Humantenmine exhibits potent neurotoxic and pharmacological activities characteristic of Gelsemium alkaloids. The compound may have analgesic, anti-inflammatory, and anxiolytic effects at low doses. Its specific in vitro activities depend on the concentrations tested and the assay systems used. Further studies are needed to fully characterize its activity profile, including its receptor binding and signaling effects. |
| ln Vivo |
In vivo studies of humantenmine are limited. Gelsemium alkaloids are known for their potent neurotoxic effects and have been used in traditional medicine for their analgesic and anti-inflammatory properties. The compound may be evaluated in animal models of pain, inflammation, and anxiety. However, due to its narrow therapeutic index, careful dose optimization is required. Further studies are needed to characterize its specific in vivo pharmacokinetic and pharmacodynamic properties.
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| Enzyme Assay |
Non-cell-based assays for humantenmine include receptor binding assays using glycine receptors, GABA receptors, or opioid receptors. Radioligand binding assays can be used to assess the compound's affinity for these receptors. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization and purity assessment.
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| Cell Assay |
Cell-based assays for humantenmine use various cell lines to assess its biological activities. Receptor activation can be assessed by measuring downstream signaling (e.g., calcium influx for glycine receptors, cAMP modulation for opioid receptors). Neuronal cell lines can be used to study effects on neuronal excitability and neurotransmitter release. Cytotoxicity is assessed using standard cell viability assays.
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| Animal Protocol |
In vivo studies of humantenmine are limited. Based on its biological activities, potential animal models include: pain models for analgesic evaluation; inflammation models for anti-inflammatory evaluation; and anxiety models for anxiolytic evaluation. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints. Due to the potent neurotoxicity of Gelsemium alkaloids, careful dose optimization is required.
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| ADME/Pharmacokinetics |
Humantenmine (Gelsenicine) has a molecular formula of C₂₀H₂₂N₂O₃ and a molecular weight of approximately 338.40 g/mol. The CAS number is 82354-38-9. It is a naturally occurring alkaloid found in plants of the Gelsemium genus. Gelsemium alkaloids are known for their potent neurotoxic and pharmacological activities. The compound should be stored under recommended conditions. It is intended for research use only.
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| Toxicity/Toxicokinetics |
Humantenmine is a potent neurotoxic alkaloid. Gelsemium alkaloids have a narrow therapeutic index and can be toxic at high doses. Symptoms of toxicity include respiratory depression, muscle weakness, and seizures. The compound should be handled with extreme caution using appropriate personal protective equipment. It is intended for research use only and is not for human consumption.
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| References |
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| Additional Infomation |
Reports indicate that gelsemine has been found in Gelsemium elegans and Gelsemium sempervirens, and relevant data are available for reference.
Humantenmine (Gelsenicine) is a naturally occurring alkaloid compound found in plants of the Gelsemium genus, including Gelsemium elegans (heartbreak grass) and Gelsemium sempervirens (yellow jasmine). Gelsemium species have been used in traditional medicine for their analgesic, anti-inflammatory, and anxiolytic properties. However, they are also known for their potent neurotoxicity. Gelsemium alkaloids, including humantenmine, interact with glycine receptors, GABA receptors, and opioid receptors. The compound is used as a research tool for studying the pharmacology and toxicology of Gelsemium alkaloids. It is for research use only. |
| Molecular Formula |
C19H22N2O3
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|---|---|
| Molecular Weight |
326.39
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| Exact Mass |
326.163
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| CAS # |
82354-38-9
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| PubChem CID |
21123652
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| Appearance |
Off-white to light yellow solid
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
463.6±55.0 °C at 760 mmHg
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| Flash Point |
234.2±31.5 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.712
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| LogP |
3.68
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
24
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| Complexity |
591
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O=C1N(OC)C2C=CC=CC=2[C@@]21C[C@@H]1N=C([C@H]3[C@@H]1CO[C@@H]2C3)CC
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| InChi Key |
BIGABVPVCRHEES-NWPJSNQLSA-N
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| InChi Code |
InChI=1S/C19H22N2O3/c1-3-14-11-8-17-19(9-15(20-14)12(11)10-24-17)13-6-4-5-7-16(13)21(23-2)18(19)22/h4-7,11-12,15,17H,3,8-10H2,1-2H3/t11-,12+,15+,17-,19+/m1/s1
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| Chemical Name |
(1R,2S,4S,7R,8S)-6-ethyl-1'-methoxyspiro[10-oxa-5-azatricyclo[5.3.1.04,8]undec-5-ene-2,3'-indole]-2'-one
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| Synonyms |
Gelsenicine; Humantenmine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (306.38 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.66 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0638 mL | 15.3191 mL | 30.6382 mL | |
| 5 mM | 0.6128 mL | 3.0638 mL | 6.1276 mL | |
| 10 mM | 0.3064 mL | 1.5319 mL | 3.0638 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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