| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Not applicable (chemical reagent for peptide synthesis). L-Histidine benzyl ester bistosylate is a protected amino acid derivative used as a building block in peptide synthesis. It does not have a specific biological target. The compound is used to introduce histidine residues into peptides during solid-phase or solution-phase peptide synthesis. Its biological effects, if any, would be related to the peptides synthesized from it rather than the reagent itself.
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|---|---|
| ln Vitro |
L-Histidine benzyl ester bistosylate is a chemical reagent used in peptide synthesis. It is a protected form of L-histidine that allows for the selective incorporation of histidine into peptides. The compound's in vitro activity is related to its use as a synthetic intermediate rather than direct biological activity. It is used to synthesize histidine-containing peptides for research purposes.
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| ln Vivo |
In vivo studies of L-histidine benzyl ester bistosylate are not applicable as the compound is a chemical reagent rather than a pharmacologically active compound. It is used in peptide synthesis to produce peptides that may be evaluated in vivo. The compound itself is not administered to animals for therapeutic or pharmacological studies.
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| Enzyme Assay |
Non-cell-based assays for L-histidine benzyl ester bistosylate are primarily analytical in nature. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization and purity assessment. The compound is used as a reactant in peptide synthesis reactions, and its coupling efficiency and purity are assessed using standard peptide chemistry methods.
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| Cell Assay |
Cell-based assays for L-histidine benzyl ester bistosylate are not commonly performed, as the compound is a chemical reagent rather than a pharmacologically active compound. Peptides synthesized using this reagent may be tested in cell-based assays, but the reagent itself is not typically used in cell culture applications.
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| Animal Protocol |
In vivo animal studies for L-histidine benzyl ester bistosylate are not applicable as the compound is a chemical reagent rather than a pharmacologically active compound. It is used in peptide synthesis to produce compounds that may be evaluated in animal models.
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| ADME/Pharmacokinetics |
L-Histidine benzyl ester bistosylate has a molecular formula of C₂₇H₃₁N₃O₇S₂ and a molecular weight of approximately 573.68 g/mol. The CAS number is 24593-59-7. It is a protected amino acid derivative with benzyl ester and tosylate groups. The compound is used as a building block for peptide synthesis. It should be stored under recommended conditions. It is intended for research use only.
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| Toxicity/Toxicokinetics |
Specific toxicity data for L-histidine benzyl ester bistosylate are limited. As a chemical reagent, it may cause irritation to the skin, eyes, and respiratory tract. Standard laboratory safety practices should be followed when handling this compound, including the use of personal protective equipment. It is intended for research use only.
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| References |
[1]. Thirumananseri Kumarevel, et al. Allosteric activation of HutP protein, that regulates transcription of hut operon in Bacillus subtilis, mediated by various analogs of histidine. Nucleic Acids Res Suppl. 2003;(3):199-200.
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| Additional Infomation |
L-Histidine benzyl ester bistosylate is a protected amino acid derivative used in peptide synthesis and medicinal chemistry research. It consists of L-histidine with a benzyl ester protecting group at the carboxyl terminus and two tosylate counterions. The compound is used as a building block for the synthesis of histidine-containing peptides and peptide-based therapeutics. Protecting groups are essential in peptide synthesis to prevent unwanted side reactions and allow for selective deprotection at specific stages of synthesis. It is for research use only.
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| Molecular Formula |
C27H31N3O8S2
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|---|---|
| Molecular Weight |
589.68
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| Exact Mass |
589.155
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| CAS # |
24593-59-7
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| PubChem CID |
14135414
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
856.1ºC at 760mmHg
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| Vapour Pressure |
3.24E-31mmHg at 25°C
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| LogP |
6.368
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
40
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| Complexity |
473
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C1=CC=C(C=C1)COC(=O)[C@H](CC2=CN=CN2)N.CC1=CC=C(C=C1)S(=O)(=O)O.CC1=CC=C(C=C1)S(=O)(=O)O
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| InChi Key |
FQHRMEOHSXSJBX-LTCKWSDVSA-N
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| InChi Code |
InChI=1S/C13H15N3O2.2C7H8O3S/c14-12(6-11-7-15-9-16-11)13(17)18-8-10-4-2-1-3-5-10;2*1-6-2-4-7(5-3-6)11(8,9)10/h1-5,7,9,12H,6,8,14H2,(H,15,16);2*2-5H,1H3,(H,8,9,10)/t12-;;/m0../s1
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| Chemical Name |
benzyl (2S)-2-amino-3-(1H-imidazol-5-yl)propanoate;4-methylbenzenesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (423.96 mM)
H2O: 100 mg/mL (169.58 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.53 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.53 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (3.53 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6958 mL | 8.4792 mL | 16.9584 mL | |
| 5 mM | 0.3392 mL | 1.6958 mL | 3.3917 mL | |
| 10 mM | 0.1696 mL | 0.8479 mL | 1.6958 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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