| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| Other Sizes |
| Targets |
Multiple targets including antioxidant enzymes, inflammatory mediators, and immune cells. Regaloside E, as a phenylpropanoid glycoside, exhibits antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial activities. The compound may scavenge free radicals, inhibit the production of pro-inflammatory mediators, modulate immune cell function, and inhibit microbial growth.
|
|---|---|
| ln Vitro |
Regaloside E exhibits antioxidant, anti-inflammatory, immunomodulatory, and antimicrobial activities characteristic of phenylpropanoid glycosides. It may scavenge free radicals, inhibit the production of inflammatory mediators, modulate immune cell function, and inhibit microbial growth. Its specific in vitro activities depend on the concentrations tested and the assay systems used.
|
| ln Vivo |
In vivo studies of regaloside E are limited. As a phenylpropanoid glycoside from Lilium species, it may contribute to the pharmacological effects of lily extracts. Its antioxidant, anti-inflammatory, and immunomodulatory activities suggest potential benefits in various disease models. Further in vivo studies are needed to characterize its specific pharmacokinetic and pharmacodynamic properties.
|
| Enzyme Assay |
Non-cell-based assays for regaloside E include antioxidant assays such as DPPH radical scavenging, ABTS, and FRAP assays. Anti-inflammatory activity is measured by inhibition of COX-2, LOX, or cytokine production in enzyme assays. Antimicrobial activity is assessed by broth microdilution for MIC determination. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization.
|
| Cell Assay |
Cell-based assays for regaloside E use various cell lines to assess its biological activities. Macrophage cell lines (e.g., RAW 264.7) are stimulated with LPS and treated with the compound, and inflammatory mediators in culture supernatant are measured. For immunomodulatory studies, immune cells are treated with the compound and proliferation or cytokine production is assessed. Antimicrobial activity is assessed in bacterial or fungal cultures.
|
| Animal Protocol |
In vivo studies of regaloside E are limited. Based on its biological activities, potential animal models include: carrageenan-induced paw edema for anti-inflammatory evaluation; immunosuppression models for immunomodulatory evaluation; and infection models for antimicrobial evaluation. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints.
|
| ADME/Pharmacokinetics |
Regaloside E has a molecular formula of C₂₆H₃₂O₁₅ and a molecular weight of approximately 584.52 g/mol. It is a naturally occurring phenylpropanoid glycoside found in various plant species, including lilies (Lilium species). The compound is soluble in organic solvents such as DMSO and methanol. It should be stored under recommended conditions.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for regaloside E are limited. As a natural phenylpropanoid glycoside, it is generally considered to have low toxicity. Standard laboratory safety practices should be followed when handling this compound.
|
| References | |
| Additional Infomation |
Regaloside E is a naturally occurring phenylpropanoid glycoside found in various plant species, including lilies (Lilium species). Phenylpropanoid glycosides are known for their diverse biological activities, including antioxidant, anti-inflammatory, antimicrobial, and immunomodulatory effects. Regaloside E is used as a reference standard for the identification and quantification of phenylpropanoid glycosides in plant extracts and for research purposes. It is for research use only.
|
| Molecular Formula |
C20H26O12
|
|---|---|
| Molecular Weight |
458.41
|
| Exact Mass |
458.142
|
| CAS # |
123134-21-4
|
| PubChem CID |
171361125
|
| Appearance |
White to off-white solid
|
| LogP |
-0.8
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
11
|
| Heavy Atom Count |
32
|
| Complexity |
640
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(=O)OCC(COC(=O)/C=C/C1=CC(=C(C=C1)O)O)OC2C(C(C(C(O2)CO)O)O)O
|
| InChi Key |
BTRIXFBTQFTXAB-SIGDFUGISA-N
|
| InChi Code |
InChI=1S/C20H26O12/c1-10(22)29-8-12(31-20-19(28)18(27)17(26)15(7-21)32-20)9-30-16(25)5-3-11-2-4-13(23)14(24)6-11/h2-6,12,15,17-21,23-24,26-28H,7-9H2,1H3/b5-3+/t12-,15-,17-,18+,19-,20-/m0/s1
|
| Chemical Name |
[(2S)-3-acetyloxy-2-[(2S,3S,4R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxypropyl] (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
|
| Synonyms |
Regaloside E
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ≥ 50 mg/mL (109.07 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (2.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1815 mL | 10.9073 mL | 21.8145 mL | |
| 5 mM | 0.4363 mL | 2.1815 mL | 4.3629 mL | |
| 10 mM | 0.2181 mL | 1.0907 mL | 2.1815 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
