| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
Isoscoparin targets multiple pathways involved in inflammation and adipogenesis. It inhibits the production of NO and TNF-α. Its mechanism of action may be due to its ability to inhibit lipoprotein lipase and the synthesis of cholesterol.
|
|---|---|
| ln Vitro |
Isoscoparin possesses antioxidant and anti-adipogenic activities. At 2.0 µM, it significantly inhibits the production of both NO and TNF-α. It has significant cytotoxicity against K562 cells.
|
| ln Vivo |
In vivo activity data for Isoscoparin are not available.
|
| Enzyme Assay |
Non-cell-based assays for Isoscoparin could involve measuring its antioxidant activity using chemical assays such as DPPH or ABTS radical scavenging. Its ability to inhibit lipoprotein lipase or cholesterol synthesis could be measured in cell-free systems using the appropriate enzymes and substrates.
|
| Cell Assay |
Macrophages or other immune cells are treated with Isoscoparin at 2.0 µM and stimulated with LPS. The production of NO and TNF-α is measured using Griess reagent or ELISA, respectively. Inhibition of these mediators indicates anti-inflammatory activity. K562 cells can be treated with Isoscoparin to assess its cytotoxicity.
|
| Animal Protocol |
In vivo animal studies for Isoscoparin have not been reported.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for Isoscoparin are not available.
|
| Toxicity/Toxicokinetics |
Toxicological data for Isoscoparin are not available.
|
| References | |
| Additional Infomation |
Isoscoparin is a C-glycoside compound composed of hyperoside with a 1,5-anhydro-D-glucol substituent at the 6-position. It is a metabolite. It is a trihydroxyflavonoid, monomethoxyflavonoid, monosaccharide derivative, and C-glycoside compound. Its function is associated with 4',5,7-trihydroxy-3'-methoxyflavonoid. It is the conjugate acid of Isoscoparin-7-olate. Isoscoparin has been reported in Gentiana grandis, Gentiana depressa, and other organisms with relevant data.
Isoscoparin is a flavonoid with antioxidant and anti-adipogenic activities. It is a potential therapeutic agent for the prevention and treatment of obesity. |
| Molecular Formula |
C22H22O11
|
|---|---|
| Molecular Weight |
462.40
|
| Exact Mass |
462.116
|
| CAS # |
20013-23-4
|
| PubChem CID |
442611
|
| Appearance |
Off-white to light yellow solid powder
|
| Boiling Point |
818.6±65.0 °C(Predicted)
|
| Source |
Originated from plants: Gentianaceae Gentiana algida Pall.
|
| LogP |
0.1
|
| Hydrogen Bond Donor Count |
7
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
33
|
| Complexity |
744
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
COC1=C(C=CC(=C1)C2=CC(=O)C3=C(O2)C=C(C(=C3O)[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O
|
| InChi Key |
KOMUHHCFAXYRPO-DGHBBABESA-N
|
| InChi Code |
InChI=1S/C22H22O11/c1-31-13-4-8(2-3-9(13)24)12-5-10(25)16-14(32-12)6-11(26)17(19(16)28)22-21(30)20(29)18(27)15(7-23)33-22/h2-6,15,18,20-24,26-30H,7H2,1H3/t15-,18-,20+,21-,22+/m1/s1
|
| Chemical Name |
5,7-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-6-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]chromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~216.3 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1626 mL | 10.8131 mL | 21.6263 mL | |
| 5 mM | 0.4325 mL | 2.1626 mL | 4.3253 mL | |
| 10 mM | 0.2163 mL | 1.0813 mL | 2.1626 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
