| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
4″-O-Glucosylvitexin inhibits thyroid peroxidase (TPO) activity. This can produce goitrogenic and antithyroid effects similar to those of methimazole.
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|---|---|
| ln Vitro |
4″-O-Glucosylvitexin inhibits thyroid peroxidase activity. This inhibitory activity is the primary characterized biological effect.
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| ln Vivo |
In vivo activity related to thyroid function is suggested by its TPO inhibition. Specific animal data are not reported.
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| Enzyme Assay |
Thyroid peroxidase activity assays are performed using purified TPO enzyme or thyroid microsomes. The oxidation of iodide or guaiacol is measured spectrophotometrically. Inhibition by the compound is assessed in a concentration-dependent manner.
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| Cell Assay |
Thyroid follicular cell lines can be used to assess TPO inhibition and its effects on thyroid hormone production.
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| Animal Protocol |
In vivo studies would involve administering the compound to rodents and measuring thyroid hormone levels and thyroid gland morphology.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 4″-O-Glucosylvitexin are not available. The compound has solubility in DMSO at 100 mg/mL (163.79 mM). Storage at 2-8°C is recommended.
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| Toxicity/Toxicokinetics |
Toxicological data for 4″-O-Glucosylvitexin are limited. TPO inhibition suggests potential antithyroid effects at high doses.
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| References |
[1]. Lei Wen, et al. An Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC. Molecules. 2017 May 9;22(5):767.
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| Additional Infomation |
4″-O-Glucosylvitexin is a research-use-only compound. It is a major C-glycosylflavone found in millet. Its TPO inhibitory activity makes it relevant to thyroid function research.
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| Molecular Formula |
C27H30O16
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|---|---|
| Molecular Weight |
610.52
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| Exact Mass |
594.158
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| CAS # |
76135-82-5
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| PubChem CID |
5488581
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| Appearance |
White to off-white solid
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
947.3±65.0 °C at 760 mmHg
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| Flash Point |
314.7±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.771
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| LogP |
-1.62
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
43
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| Complexity |
988
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| Defined Atom Stereocenter Count |
10
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| SMILES |
c1cc(ccc1c2cc(=O)c3c(cc(c(c3o2)C4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)O)O)O)O)O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (409.49 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.41 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (3.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6379 mL | 8.1897 mL | 16.3795 mL | |
| 5 mM | 0.3276 mL | 1.6379 mL | 3.2759 mL | |
| 10 mM | 0.1638 mL | 0.8190 mL | 1.6379 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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