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| Other Sizes |
| Targets |
The biological targets of 1,5,5-Trimethylhydantoin are not well characterized in the available literature. It is classified as an "Others" target compound. As a hydantoin derivative, it may interact with enzymes or receptors involved in antimicrobial pathways. The compound's primary application is as a non-isotopic internal standard rather than as a pharmacologically active agent. Further research is needed to identify specific molecular targets and elucidate its mechanism of action.
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| ln Vitro |
In vitro biological activity of 1,5,5-Trimethylhydantoin has been reported, particularly its antimicrobial properties. However, specific potency data such as MIC values against particular microbial strains are not detailed in the available sources. The compound's activity as a hydantoin derivative suggests potential interactions with biological macromolecules. Its primary in vitro application is as an analytical standard rather than as a bioactive compound in cellular assays. The antimicrobial compounds 1-chloro-3,5,5-trimethylhydantoin and 3-chloro-1,5,5-trimethylhydantoin have been synthesized and examined.
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| ln Vivo |
Specific in vivo activity data for 1,5,5-Trimethylhydantoin are not available in the consulted sources. The compound is primarily used as an internal standard in analytical applications. No therapeutic or pharmacological in vivo studies have been reported. Its biological activities, including antimicrobial properties, have not been characterized in animal models. The compound is not intended for in vivo use and is strictly a research chemical for laboratory applications.
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| Enzyme Assay |
No specific protocols for enzyme/receptor binding assays are available for 1,5,5-Trimethylhydantoin. As an analytical internal standard, it is typically used in chromatographic methods such as GC-MS or LC-MS for quantitative analysis of hydantoin derivatives or related compounds. Standard curves are prepared using known concentrations of TMH, and the compound is added to samples at a fixed concentration to correct for variations in injection volume and instrument response. Its physicochemical properties, including solubility in dimethylformamide, make it suitable for such applications.
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| Cell Assay |
No specific cell-based assay protocols are available for 1,5,5-Trimethylhydantoin. The compound's primary use as a non-isotopic internal standard does not involve cellular assays. For antimicrobial testing, standard broth microdilution methods could be employed to determine MIC values against bacterial or fungal strains, but such data are not reported in the available sources. The compound is not typically used in mammalian cell-based assays due to its primary application as an analytical standard.
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| Animal Protocol |
No specific in vivo animal experiment protocols are available for 1,5,5-Trimethylhydantoin. The compound is not used in pharmacological or toxicological studies in animals. Its application as an internal standard is limited to analytical chemistry. No pharmacokinetic or efficacy studies in animal models have been reported. The compound is intended for research use only and is not administered to animals for therapeutic purposes.
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| ADME/Pharmacokinetics |
Specific pharmacokinetic data for 1,5,5-Trimethylhydantoin are not available in the consulted sources. As a small, polar molecule with a molecular weight of 142.16 g/mol, it would be expected to have good aqueous solubility and rapid renal excretion. However, no ADME studies have been reported. Its use as an internal standard suggests it is stable and detectable in biological matrices. The compound's physicochemical properties, including its melting point of 161-164°C and density of 1.2298 g/cm³, are well characterized.
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| Toxicity/Toxicokinetics |
Toxicological data for 1,5,5-Trimethylhydantoin are not available in the consulted sources. As a hydantoin derivative, it may have low acute toxicity, but comprehensive toxicological studies have not been reported. The compound's primary use as an analytical standard does not require extensive toxicological characterization. However, standard laboratory safety precautions should be followed when handling the compound. It is not intended for human use and is for research purposes only.
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| References |
[1]. Zi-Ao Huang, et al. Application of multisegment injection on quantification of creatinine and standard addition analysis of urinary 5-hydroxyindoleacetic acid simultaneously with creatinine normalization. Electrophoresis. 2020 Feb;41(3-4):183-193.
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| Additional Infomation |
1,5,5-Trimethylhydantoin (TMH) is primarily used as a non-isotopic internal standard for analytical applications. It is a hydantoin derivative with antimicrobial properties and potential applications in drug development. The compound has a density of 1.2298 g/cm³ and is soluble in dimethylformamide. It is not approved for any therapeutic indication and is strictly a research chemical. The antimicrobial compounds derived from TMH have been synthesized and examined via joint experimental and computational studies.
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| Molecular Formula |
C6H10N2O2
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|---|---|
| Molecular Weight |
142.16
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| Exact Mass |
142.074
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| CAS # |
6851-81-6
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| PubChem CID |
81295
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| Appearance |
White to off-white solid powder
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| Density |
1.117 g/cm3
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| Melting Point |
161-164 °C(lit.)
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| Index of Refraction |
1.463
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| LogP |
0.213
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
10
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| Complexity |
198
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C(C([H])([H])[H])(C([H])([H])[H])N(C([H])([H])[H])C(N1[H])=O
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| InChi Key |
ZNYIPTYJBRGSSL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H10N2O2/c1-6(2)4(9)7-5(10)8(6)3/h1-3H3,(H,7,9,10)
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| Chemical Name |
1,5,5-trimethylimidazolidine-2,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (703.43 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (17.59 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (17.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (17.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.0343 mL | 35.1716 mL | 70.3433 mL | |
| 5 mM | 1.4069 mL | 7.0343 mL | 14.0687 mL | |
| 10 mM | 0.7034 mL | 3.5172 mL | 7.0343 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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