| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| Other Sizes |
| Targets |
Imidaclothiz functions as an agonist at insect nicotinic acetylcholine receptors (nAChRs). It targets the nicotinic acid acetylcholinesterase enzyme acceptor. By binding to nAChRs, it disrupts neurotransmission in insects, leading to paralysis and death. It exhibits contact activity.
|
|---|---|
| ln Vitro |
In vitro, Imidaclothiz exhibits excellent insecticidal efficacy. It is a neonicotinoid insecticide used for research on sucking and biting insects. It shows activity as an agonist at insect nicotinic acetylcholine receptors. Detailed in vitro data are limited.
|
| ln Vivo |
In vivo, Imidaclothiz has excellent insecticidal effect. It can be utilized in the study of sucking and biting insects. It is used as a pesticide. Its efficacy has been demonstrated in agricultural applications.
|
| Enzyme Assay |
Cell-free assays for Imidaclothiz: nicotinic acetylcholine receptor binding is assessed using radioligand binding assays with membrane preparations from insect tissues. The compound is incubated with receptor membranes and radiolabeled ligands, and specific binding is measured by scintillation counting. IC50 values are calculated from displacement curves.
|
| Cell Assay |
Cellular assays for Imidaclothiz: insect cell lines or primary neurons are treated with Imidaclothiz at concentrations of 0.1-100 µM for 1-24 hours. Receptor-mediated signaling (e.g., calcium flux) is measured. Cell viability is assessed. Insecticidal activity is evaluated by exposing insects to the compound and measuring mortality.
|
| Animal Protocol |
In vivo animal studies for Imidaclothiz: insect models (e.g., aphids, planthoppers) are used to evaluate insecticidal efficacy. Imidaclothiz is applied topically or systemically at various concentrations. Mortality, feeding behavior, and other parameters are assessed. Toxicity to non-target organisms is also evaluated.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Imidaclothiz: as a small molecule (molecular weight ~272), it may have good bioavailability in insects. In mammals, it is likely metabolized in the liver and excreted via the kidneys. Environmental fate and persistence are also relevant for its use as a pesticide.
|
| Toxicity/Toxicokinetics |
Toxicity of Imidaclothiz: as a neonicotinoid insecticide, it may have toxicity to non-target organisms, including beneficial insects and aquatic life. It should be handled with caution. It is for research use only and not for human therapeutic use.
|
| References |
[1]. Tao Y, et, al. Uptake, Translocation, and Biotransformation of Neonicotinoid Imidaclothiz in Hydroponic Vegetables: Implications for Potential Intake Risks. J Agric Food Chem. 2021 Apr 14;69(14):4064-4073.
|
| Additional Infomation |
Imidaclothiz is a neonicotinoid insecticide used in agricultural research and pest control. It is approved for use in China. Its mechanism involves agonism at insect nicotinic acetylcholine receptors. No clinical applications have been reported.
|
| Molecular Formula |
C7H8CLN5O2S
|
|---|---|
| Molecular Weight |
261.69
|
| Exact Mass |
261.008
|
| CAS # |
105843-36-5
|
| PubChem CID |
184601
|
| Appearance |
White to off-white solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
461.7±55.0 °C at 760 mmHg
|
| Melting Point |
146-147 ºC
|
| Flash Point |
233.0±31.5 °C
|
| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
| Index of Refraction |
1.800
|
| LogP |
-0.84
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
16
|
| Complexity |
310
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=[N+](NC1=NCCN1CC1=CN=C(Cl)S1)[O-]
|
| InChi Key |
OWRSHPAYDYCHSJ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H8ClN5O2S/c8-6-10-3-5(16-6)4-12-2-1-9-7(12)11-13(14)15/h3H,1-2,4H2,(H,9,11)
|
| Chemical Name |
N-[1-[(2-chloro-1,3-thiazol-5-yl)methyl]-4,5-dihydroimidazol-2-yl]nitramide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (382.13 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.55 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.55 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8213 mL | 19.1066 mL | 38.2132 mL | |
| 5 mM | 0.7643 mL | 3.8213 mL | 7.6426 mL | |
| 10 mM | 0.3821 mL | 1.9107 mL | 3.8213 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
