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Gp100 (25-33), human TFA

Alias: Hgp100 (25-33) TFA
Cat No.:V136850 Purity: ≥98%
Gp100 (25-33), human TFA, is the amino acid fragment at positions 25-33 of the human melanoma antigen.
Gp100 (25-33), human TFA
Gp100 (25-33), human TFA Chemical Structure Product category: Others 17
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
Other Sizes

Other Forms of Gp100 (25-33), human TFA:

  • Gp100 (25-33), human TFA (Hgp100 (25-33) (TFA))
  • Gp100 (25-33), human
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Top Publications Citing lnvivochem Products
Product Description
Gp100 (25-33), human TFA, is the amino acid fragment at positions 25-33 of the human melanoma antigen. Gp100 (25-33) is a 9-amino acid epitope, restricted by MHC class I molecule H-2Db, and can be recognized by T cells. Gp100 (25-33) is immunogenic and can induce the production of specific T cells. Gp100 (25-33) can be used in cancer research, such as melanoma research.
Biological Activity I Assay Protocols (From Reference)
ln Vitro
Gp100 (25-33), human TFA (recombinant vaccinia virus (rVV) encoding human gp100) (24 hours) produces CD4-CD8+ lysing T lymphocytes in spleen cells[1].
ln Vivo
Gp100 (25-33), human TFA (rVVgp100-induced T cells) (intraperitoneal injection, twice daily for 5 days) showed antitumor effects in a B16 tumor mouse model [1].
References

[1]. gp100/pmel 17 is a murine tumor rejection antigen: induction of "self"-reactive, tumoricidal T cells using high-affinity, altered peptide ligand. J Exp Med. 1998 Jul 20;188(2):277-86.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C54H83F3N16O16
Molecular Weight
1269.33
Related CAS #
Gp100 (25-33), human
Sequence
Lys-Val-Pro-Arg-Asn-Gln-Asp-Trp-LeuKVPRNQDWL
SequenceShortening
KVPRNQDWL
Appearance
White to off-white solid
Synonyms
Hgp100 (25-33) TFA
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Note: 请将本产品存放在密封且受保护的环境中(例如氮气下),避免暴露在潮湿和光照下。
Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
H2O : ~100 mg/mL (~78.78 mM; with sonication)
Solubility (In Vivo)
Solubility in Formulation 1: 66.67 mg/mL (52.52 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), Clear solution; Need ultrasonic.

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 0.7878 mL 3.9391 mL 7.8782 mL
5 mM 0.1576 mL 0.7878 mL 1.5756 mL
10 mM 0.0788 mL 0.3939 mL 0.7878 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
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