| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| ln Vitro |
AqF026 (0.1-100 μM) can directly enhance human AQP1-mediated water transport in Xenopus oocytes, with an EC50 of 3.3 μM [1].
|
|---|---|
| ln Vivo |
AqF026 (0.75-30 μM; intravenous injection; single injection 30 minutes before dialysis test) can directly and specifically enhance AQP1-mediated osmotic water transport in a mouse peritoneal dialysis model. At 15 μM, it can increase net ultrafiltration by up to 15%, with an EC50 of 4.2 μM, and has no effect on solute transport or AQP1 expression [1].
|
| Animal Protocol |
Animal/Disease Models:Wild-type (Aqp1+/+) (same littermate mice, 8-12 weeks old); Aqp1 knockout (Aqp1-/-) (same littermate mice, 8-12 weeks old) [1]
Doses: 0.75 μM; 1.5 μM; 7.5 μM; 15 μM; 30 μM Route of Administration: Intravenous injection; single injection 30 minutes before dialysis test Experimental Results: In wild-type mice, net ultrafiltration increased in a dose-dependent manner, with a maximum increase of 15% at 15 μM, and an estimated EC50 of 4.2 μM. In wild-type mice, the initial ultrafiltration rate increased by about 50% (32.2 μL/min at 15 μM). At a concentration of 15 μM, the intraperitoneal fluid volume increased significantly at 30, 60 and 90 minutes. In wild-type mice, sodium sieving was enhanced, with a greater decrease in dialysate sodium concentration at 30 minutes (11.2 mEq/L) at 15 μM concentration. There was no effect on net or initial ultrafiltration rate in Aqp1 knockout mice. In wild-type mice, there were no alterations to osmotic gradient, small solute (urea and glucose) transport, AQP1 protein/mRNA expression, or AQP1 plasma membrane localization. |
| References |
| Molecular Formula |
C20H19CLN2O5S
|
|---|---|
| Molecular Weight |
434.89
|
| CAS # |
1021869-59-9
|
| Appearance |
White to off-white solid
|
| SMILES |
O=C(C1=CC(S(=O)(NCC2=CC=CC=C2)=O)=C(C=C1NCC3=CC=CO3)Cl)OC
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~229.94 mM; with sonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.75 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in double-distilled water and dilute to 100 mL to obtain clear physiological saline. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.75 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2994 mL | 11.4972 mL | 22.9943 mL | |
| 5 mM | 0.4599 mL | 2.2994 mL | 4.5989 mL | |
| 10 mM | 0.2299 mL | 1.1497 mL | 2.2994 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
