| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| ln Vitro |
ADAR1i-124 (0-200 μM; 2 h) effectively inhibited ADAR1p150-mediated A-to-I RNA editing in vitro, with IC50 values of 8.8 μM (position 2) and 6.5 μM (position 5), respectively [1]. ADAR1i-124 (300 μM; 2 h) did not inhibit ADAR2-mediated A-to-I RNA editing in vitro [1]. ADAR1i-124 selectively binds to ADAR1p110 with a higher affinity than ADAR2 and does not bind to GAPDH [1]. ADAR1i-124 (10 μM; 12 h) induced unedited double-stranded RNA co-localized with MDA5 in the filamentous structure of mouse Yumm1.7 cutaneous melanoma cells [1]. ADAR1i-124 (10 μM; 12 h) reduced A-to-I editing in Yumm1.7 cells and promoted the conversion of certain double-stranded RNAs from type A to type Z [1]. ADAR1i-124 (48 h) showed dose-dependent cytotoxicity against mouse (Yumm1.7 CM, HSG2 OC) and human (WM4223 AM, WM3000 CM) cancer cells, with IC50 values of 2.3, 1.2, 1.1 and 6.6 μM, respectively [1]. ADAR1i-124 (10 μM; 24 h) activated the IFN signaling pathway in Yumm1.7 melanoma cells and HGS2 OC cells [1]. ADAR1i-124 (10 μM; 24 h) can induce apoptosis in HeLa and Yumm1.7 cells, and can also induce necrotic apoptosis in Yumm1.7 cells activated by the ZBP1 pathway [1].
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| Cell Assay |
Real-time quantitative PCR[1]
Cell Types: Yumm1.7 melanoma cells and HGS2 ovarian cancer cells Tested Concentrations: 10 μM Incubation Duration: 24 h Experimental Results: The expression of IFN-stimulated genes such as Ifih1 (MDA5), Cxcl9 and Cxcl10 was upregulated. |
| References |
| Molecular Formula |
C17H12CLN3O2
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|---|---|
| Molecular Weight |
325.75
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| Appearance |
White to off-white solid
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| SMILES |
OCN1C(C(NC2=CC=C(C#N)C=C2)=O)=C(Cl)C3=C1C=CC=C3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: 本产品在运输和储存过程中需避光（避免光照）。 |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~306.98 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in double-distilled water and dilute to 100 mL to obtain clear physiological saline. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0698 mL | 15.3492 mL | 30.6984 mL | |
| 5 mM | 0.6140 mL | 3.0698 mL | 6.1397 mL | |
| 10 mM | 0.3070 mL | 1.5349 mL | 3.0698 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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