| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Proanthocyanidin A4 targets multiple cellular pathways involved in inflammation and oxidative stress. It scavenges free radicals and regulates signaling pathways related to inflammation and cell survival. The compound modulates immune responses and protects cells against oxidative damage. Its antioxidant activity is attributed to its ability to scavenge free radicals and chelate metal ions. The compound's anti-inflammatory effects are mediated through modulation of inflammatory signaling pathways.
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| ln Vitro |
In vitro, proanthocyanidin A4 demonstrates strong antioxidant properties by scavenging free radicals and protecting cells against oxidative stress. It shows anti-inflammatory activity by modulating immune responses and reducing inflammatory mediators. The compound exhibits antimicrobial properties against various pathogens. Its anticancer potential has been investigated in cancer cell lines, where it shows cytotoxic effects. The compound's ability to regulate signaling pathways makes it a valuable bioactive compound for research.
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| ln Vivo |
In vivo, proanthocyanidin A4 has been studied for its potential health benefits including cardiovascular protection, anticancer effects, and neuroprotection. Its antioxidant and anti-inflammatory properties suggest it may protect against various chronic diseases. The compound is found naturally in plants and is consumed as part of the diet. Studies have investigated its effects on oxidative stress markers, inflammatory parameters, and tissue protection in animal models.
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| Enzyme Assay |
For non-cell enzyme/receptor binding assays, proanthocyanidin A4 antioxidant activity is assessed using cell-free radical-scavenging assays such as DPPH, ABTS, and FRAP. The compound's ability to scavenge free radicals is measured spectrophotometrically. Its metal-chelating activity can also be assessed. The compound's interactions with inflammatory signaling molecules can be studied using biochemical assays and surface plasmon resonance.
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| Cell Assay |
For in vitro cell-based assays, proanthocyanidin A4 is tested in various cell types including cancer cells, immune cells, and neuronal cells. Its antioxidant activity is measured by assessing protection against oxidative stress-induced cell damage. Anti-inflammatory activity is evaluated by measuring cytokine production and inflammatory marker expression. Cytotoxicity and antiproliferative effects are assessed in cancer cell lines. Neuroprotective effects are studied in neuronal cell models.
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| Animal Protocol |
For in vivo animal experiments, proanthocyanidin A4 can be administered orally or intraperitoneally to rodent models. Its effects on cardiovascular health are assessed in models of cardiovascular disease. Anticancer activity is evaluated in tumor-bearing animals. Neuroprotective effects are studied in models of neurodegenerative diseases. Oxidative stress markers, inflammatory parameters, and tissue pathology are assessed following compound administration.
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| ADME/Pharmacokinetics |
Proanthocyanidin A4 has a molecular weight of 576.50 g/mol and molecular formula C₃₀H₂₄O₁₂. It is a polyphenolic compound with multiple hydroxyl groups. The compound should be stored at -20°C for long-term stability. It is a natural product isolated from plant sources with purity ≥95% available for research purposes. The compound is soluble in organic solvents and has characteristic polyphenolic properties.
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| Toxicity/Toxicokinetics |
Proanthocyanidin A4 is a natural polyphenol found in various plants and is considered safe at dietary levels. It is consumed as part of the diet through plant-based foods. No significant toxicity has been reported at physiological concentrations. The compound is for research use only and is not approved as a pharmaceutical drug. Standard safety precautions for handling natural products should be followed. Its antioxidant properties suggest it may have health benefits.
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| References | |
| Additional Infomation |
Proanthocyanidin A4 is a naturally occurring type A proanthocyanidin found in various plants including E. sinica roots. It exhibits strong antioxidant, anti-inflammatory, and antimicrobial properties. The compound has been studied for its potential health benefits including cardiovascular protection, anticancer activity, and neuroprotection. Its ability to scavenge free radicals and regulate signaling pathways makes it a valuable bioactive compound for nutritional and pharmacological research. No clinical trials have been conducted for this compound as a therapeutic agent.
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| Molecular Formula |
C30H24O13
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|---|---|
| Molecular Weight |
592.503769874573
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| Exact Mass |
576.127
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| CAS # |
111466-29-6
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| PubChem CID |
53349182
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| Appearance |
White to off-white solid powder
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| Density |
1.766±0.06 g/cm3
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| LogP |
2.793
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
43
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| Complexity |
1010
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C1[C@@H]([C@H](OC2=C1C(=CC3=C2[C@H]4[C@@H]([C@@](O3)(OC5=CC(=CC(=C45)O)O)C6=CC(=C(C(=C6)O)O)O)O)O)C7=CC(=C(C=C7)O)O)O
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| InChi Key |
WODBGULXKVZGQF-WWFYOJLTSA-N
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| InChi Code |
InChI=1S/C30H24O13/c31-12-6-17(35)23-21(7-12)42-30(11-4-18(36)26(39)19(37)5-11)29(40)25(23)24-22(43-30)9-15(33)13-8-20(38)27(41-28(13)24)10-1-2-14(32)16(34)3-10/h1-7,9,20,25,27,29,31-40H,8H2/t20-,25-,27+,29-,30+/m0/s1
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| Chemical Name |
(1S,5R,6S,13R,21S)-5-(3,4-dihydroxyphenyl)-13-(3,4,5-trihydroxyphenyl)-4,12,14-trioxapentacyclo[11.7.1.02,11.03,8.015,20]henicosa-2(11),3(8),9,15,17,19-hexaene-6,9,17,19,21-pentol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 50 mg/mL (~86.73 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (2.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6878 mL | 8.4388 mL | 16.8776 mL | |
| 5 mM | 0.3376 mL | 1.6878 mL | 3.3755 mL | |
| 10 mM | 0.1688 mL | 0.8439 mL | 1.6878 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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