| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| ln Vitro |
Stimulation of CB1 receptors raises brain pregnenolone levels, which counteracts most of the known physical and behavioral effects of THC by producing negative feedback on CB1 receptor activity. Pregnenolone binds to a different location than the orthosteric ligand, potentially acting as a distinct negative allosteric modulator of signaling. Pregnenolone solely affects agonist efficacy; it has no effect on agonist binding [1]. Slices that had a 100 nM pregnenolone pretreatment showed a considerable reduction in THC's impact (inhibition rate of 15.1±1.8%). Pregnenolone's presynaptic actions could be the cause of these results. Pregnenolone, therefore, does not change the amplitude or decay duration of micro-EPSCs (mEPSCs), but it does suppress the THC-induced rise in paired pulse ratio (PPR) [1].
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| ln Vivo |
Pregnenolone treatment (2-6 mg/kg) reduces THC-induced food intake in Wistar rats and C57BL/6N mice and attenuates THC-induced memory impairment in mice, but does not change itself these behaviors. Injection of pregnenolone (2 and 4 mg/kg) before each self-administration reduces WIN 55,212-2 consumption and reduces breakpoints in a graduated-ratio regimen [1].
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| References |
[1]. Vallée M, et al. Pregnenolone can protect the brain from cannabis intoxication. Science. 2014 Jan 3;343(6166):94-8.
[2]. Ducharme N, et al. Brain distribution and behavioral effects of progesterone and pregnenolone after intranasal or intravenous administration. Eur J Pharmacol. 2010 Sep 1;641(2-3):128-34. [3]. Alan Shiels. TRPM3_miR-204: a complex locus for eye development and disease. Hum Genomics. 2020 Feb 18;14(1):7 |
| Molecular Formula |
C21H31NAO5S
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|---|---|
| Molecular Weight |
418.52
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| Exact Mass |
418.179
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| CAS # |
1852-38-6
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| Related CAS # |
Pregnenolone;145-13-1;Pregnenolone monosulfate;1247-64-9;Pregnenolone monosulfate sodium-13C2,d2;2483824-22-0;Pregnenolone monosulfate-d4 sodium;1485492-21-4
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| SMILES |
[Na+].O=S(OC1CCC2(C3CCC4(C(CCC4C3CC=C2C1)C(=O)C)C)C)(=O)[O-]
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| Synonyms |
Pregnenolone sulfate sodium salt; Pregnenolone sulfate sodium
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~238.94 mM)
H2O : ~5 mg/mL (~11.95 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.97 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.97 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3894 mL | 11.9469 mL | 23.8937 mL | |
| 5 mM | 0.4779 mL | 2.3894 mL | 4.7787 mL | |
| 10 mM | 0.2389 mL | 1.1947 mL | 2.3894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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