| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
With epidermal activity, pregnenolone 16α-carbonitrile is a powerful cryo-PXR activator that is the embodiment of perfection. Synthetic obese, pregnenolone 16α-carbonitrile, has been shown to promote resistance to stress shocks [1][2][3]. It also increases the production of cytochrome P450 3A.
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|---|---|
| ln Vivo |
Pregnenolone 16α-carbonitrile (40 mg/kg/day; ip; for two days) stimulates the expression of Cyp3a11 and Cyp2b10 at the mRNA, protein and enzyme levels in WT mice [1]. Pregnenolone 16α-carbonitrile (100 mg/kg) Pregnenolone 16α-carbonitrile (35 mg/kg; gavage; once a day for three consecutive days) enhances the expression of Sprague-Dawley CYP3A mRNA in males and females weighing 150 -200 g [2].
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| Animal Protocol |
Animal/Disease Models: WT and Pxr-/- mice [1]
Doses: 40 mg/kg Route of Administration: IP; -Dawley deposited Pgp expression, age approximately 100 days, body weight 250-400 grams [3]. one time/day for two days Experimental Results: Induced expression of Cyp3a11 and Cyp2b10 at the mRNA, protein and enzyme levels in WT mice. Little effect on the expression of Cyp3a11 in Pxr-/- mice |
| References |
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| Additional Infomation |
Pregnenolone 16alpha-carbonitrile is an aliphatic nitrile. It is functionally related to a pregnenolone.
A catatoxic steroid and microsomal enzyme inducer having significant effects on the induction of cytochrome P450. It has also demonstrated the potential for protective capability against acetaminophen-induced liver damage. |
| Molecular Formula |
C22H31NO2
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|---|---|
| Molecular Weight |
341.48704
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| Exact Mass |
341.235
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| CAS # |
1434-54-4
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| PubChem CID |
15032
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
508.5±50.0 °C at 760 mmHg
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| Flash Point |
261.3±30.1 °C
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| Vapour Pressure |
0.0±3.0 mmHg at 25°C
|
| Index of Refraction |
1.558
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| LogP |
3.22
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
25
|
| Complexity |
673
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| Defined Atom Stereocenter Count |
8
|
| SMILES |
CC(=O)[C@H]1[C@@H](C[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C)C#N
|
| InChi Key |
VSBHRRMYCDQLJF-ZDNYCOCVSA-N
|
| InChi Code |
InChI=1S/C22H31NO2/c1-13(24)20-14(12-23)10-19-17-5-4-15-11-16(25)6-8-21(15,2)18(17)7-9-22(19,20)3/h4,14,16-20,25H,5-11H2,1-3H3/t14-,16-,17+,18-,19-,20-,21-,22-/m0/s1
|
| Chemical Name |
(3S,8S,9S,10R,13S,14S,16R,17S)-17-Acetyl-3-hydroxy-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-16-carbonitrile
|
| Synonyms |
Pregnenolone-16alpha-carbonitrile SC4674 SC 4674 SC-4674 PCNPregnenolone-carbonitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~20 mg/mL (~58.57 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.43 mg/mL (4.19 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (4.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9283 mL | 14.6417 mL | 29.2834 mL | |
| 5 mM | 0.5857 mL | 2.9283 mL | 5.8567 mL | |
| 10 mM | 0.2928 mL | 1.4642 mL | 2.9283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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