Polydatin

Alias: Polydatin; Resveratrol 3-O-β-D-Glucopyranoside;Reservatrol 3-β-mono-D-Glucoside; Trihydroxystilbene-3-β-D-Glucopyranoside; Piceid;
Cat No.:V0842 Purity: ≥98%
Polydatin (Reservatrol 3-β-mono-D-Glucoside; Piceid)is a naturally occuring stilbene and aprecursor of resveratrolas well as a crystal component extracted from the root stem of perennial herbage Polygonum Cuspidatum Sieb.
Polydatin Chemical Structure CAS No.: 65914-17-2
Product category: Phospholipase
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
500mg
1g
2g
Other Sizes
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Polydatin (Reservatrol 3-β-mono-D-Glucoside; Piceid) is a naturally occuring stilbene and a precursor of resveratrol as well as a crystal component extracted from the root stem of perennial herbage Polygonum Cuspidatum Sieb.et Zucc. It is also known as a PLA2 inhibitor which reduces Phospholipase A2 (PLA2) activity and sPLA2-IIA mRNA expression and mitigates LPS-induced lung injury by increasing increased Clara cell secretory protein (CCSP) expression.

Biological Activity I Assay Protocols (From Reference)
ln Vitro

In vitro activity: Polydatin, known as a PLA2 inhibitor, reduces Phospholipase A2 (PLA2) activity and sPLA2-IIA mRNA expression and mitigates LPS-induced lung injury by increasing increased Clara cell secretory protein (CCSP) expression.


Cell Assay: The effect of polydatin on mMEC viability is evaluated with an MTT assay. mMECs are incubated in the presence or absence of various concentrations of polydatin (25, 50 and 100 μg/mL) and DEX (100 μg/mL) for 24 h. Next, 20 μL of MTT (5 mg/mL) is added to each well and incubated for 4 h. After the supernatants are removed and the formazan is dis¬solved with 150 μL of DMSO in each well, the optical density (OD) value is measured at 570 nm on a microplate reader

ln Vivo
Pretreatment with Polydatin (15, 45, and 100 mg/kg) dose-dependently reduces sepsis-induced mortality and lung injury in cecal ligation and puncture (CLP-)induced sepsis mice through inhibiting CLP-induced serum tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) production, lung cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase isoform (iNOS) protein expressions and NF-kappaB activation. LD50: Mice 1g/kg (i.p.).
Animal Protocol

Rats: Rats are divided into six groups by random assignment and treated as follows: in normal group and polydatin control group: rats are administrated with CMC-Na and polydatin (200 μmol/kg) by gavage respectively, and given normal saline (NS) by tail intravenous (iv) injection with the same volume; in DOX group: rats are injected with DOX by cauda vein for 4 weeks (3 mg/kg per week), the cumulative dosage is 12 mg/kg similar to that in the research of Chang et al.

Mice: Polydatin is dissolved in dimethyl sulfoxide (DMSO) and diluted in Dulbecco’s modified Eagle’s medium (DMEM). Sixty adult female postpartum and lactating BALB/c mice (6–8 weeks old, weighing 35–40 g) are obtained. Control group (CG): The mice are treated with 100 μL of PBS as a vehicle control. Polydatin groups: 24h after S aureus infection, the mouse model of S aureus mastitis is intraperitoneally administered polydatin at 15, 30 and 45 mg/kg

Rats and Mice
References
BMC Cell Biol.2011 Jul 25;12:31;Mediators Inflamm.2013;2013:354087.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C20H22O8
Molecular Weight
390.38
CAS #
65914-17-2
SMILES
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[H])O[H])O[H])O[H])OC1C([H])=C(C([H])=C(/C(/[H])=C(\[H])/C2C([H])=C([H])C(=C([H])C=2[H])O[H])C=1[H])O[H]
Synonyms
Polydatin; Resveratrol 3-O-β-D-Glucopyranoside;Reservatrol 3-β-mono-D-Glucoside; Trihydroxystilbene-3-β-D-Glucopyranoside; Piceid;
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 78 mg/mL (199.8 mM)
Water:<1 mg/mL
Ethanol:<1 mg/mL
Solubility (In Vivo)

Chemical Name: (2S,3R,4S,5S,6R)-2-(3-hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol

InChi Key: HSTZMXCBWJGKHG-CUYWLFDKSA-N

InChi Code: InChI=1S/C20H22O8/c21-10-16-17(24)18(25)19(26)20(28-16)27-15-8-12(7-14(23)9-15)2-1-11-3-5-13(22)6-4-11/h1-9,16-26H,10H2/b2-1+/t16-,17-,18+,19-,20-/m1/s1

SMILES Code: O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1CO)OC2=CC(C=CC3=CC=C(O)C=C3)=CC(O)=C2

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.5616 mL 12.8080 mL 25.6161 mL
5 mM 0.5123 mL 2.5616 mL 5.1232 mL
10 mM 0.2562 mL 1.2808 mL 2.5616 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
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Biological Data
  • Polydatin

    Histology changes and edema in the lungs from the treated-mice.Mediators Inflamm.2013;2013:354087.
  • Polydatin

    Effects of PD on the protein expression (a) and activity (b) of HO in the lungs of mice.Mediators Inflamm.2013;2013:354087.

  • Polydatin
    Effects of PD on the protein expression of COX-2 and iNOS (a), and PGE2 (b), and NO (c) production in the lungs of mice.Mediators Inflamm.2013;2013:354087.
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