| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Poly-L-lysine HBr does not have a specific molecular target but rather functions through electrostatic interactions. The positively charged polymer binds to negatively charged cell surface components such as sialic acid residues and other anionic molecules on the cell membrane. This enhances cell adhesion to solid substrates, promoting cell spreading and survival. The polymer also interacts with negatively charged culture surfaces, creating a bridge between the substrate and cells. As a polymer, it does not target a specific receptor or enzyme but serves as a general adhesion promoter.
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| ln Vitro |
In vitro, Poly-L-lysine HBr is used as a coating reagent for cell culture substrates to promote cell adhesion. It is particularly useful for culturing neurons, primary cells, and other cell types that are difficult to attach to uncoated surfaces. The polymer enhances electrostatic interactions between cells and the culture surface, improving cell attachment, spreading, and survival. It is also used to coat glass slides for immunohistochemistry and other applications requiring cell or tissue adhesion.
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| ln Vivo |
In vivo, Poly-L-lysine HBr is not typically used as a therapeutic agent. It is primarily used in research applications for cell culture and tissue preparation. The polymer can be used to coat implantable devices or scaffolds to improve cell attachment in tissue engineering applications, but its use in vivo would be limited by its foreign-body nature and potential for immune responses. Its primary applications are in vitro for cell culture and histological preparations.
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| Enzyme Assay |
For cell culture substrate coating, Poly-L-lysine HBr is dissolved in sterile water or PBS at concentrations of 0.01-1 mg/mL, depending on the cell type and application. The coating solution is added to culture plates, coverslips, or glass slides and incubated for 1-24 hours at room temperature or 37°C. The coated surfaces are then washed with sterile water or PBS and allowed to air dry before use. Coated surfaces can be stored at 4°C for several weeks.
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| Cell Assay |
For cell adhesion assays, cells are seeded onto Poly-L-lysine-coated surfaces and cultured under standard conditions. Cell attachment is assessed by washing away non-adherent cells and quantifying adherent cells by staining (crystal violet) or by measuring cell viability (MTT, CCK-8). The polymer's effectiveness can be evaluated by comparing cell attachment on coated versus uncoated surfaces. Neurite outgrowth assays can also be performed on coated surfaces to study neuronal differentiation.
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| Animal Protocol |
For in vivo applications, Poly-L-lysine-coated scaffolds or implants can be used in tissue engineering studies. Scaffolds are coated with Poly-L-lysine to improve cell seeding efficiency and tissue integration. In animal models, coated scaffolds are implanted, and tissue formation is assessed by histology and immunohistochemistry. However, specific in vivo protocols for Poly-L-lysine HBr have not been detailed in the available literature.
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| ADME/Pharmacokinetics |
Poly-L-lysine HBr is a water-soluble polymer that is stable as a solid. The molecular weight range of 70,000-150,000 provides optimal cell attachment properties. The polymer should be stored at 4°C or -20°C for long-term stability. Solutions should be sterile-filtered before use to avoid contamination. The compound is for research use only and is not intended for human or veterinary use.
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| Toxicity/Toxicokinetics |
As a polymer used for cell culture substrate coating, Poly-L-lysine HBr is generally considered non-toxic at the concentrations used for coating applications (0.01-1 mg/mL). However, exposure to high concentrations or ingestion may cause irritation. Standard laboratory safety precautions should be followed when handling this compound. It is not intended for therapeutic use.
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| References | |
| Additional Infomation |
Lys-Lys-Lys is an oligopeptide.
See also: Poly(L-lysine) hydrobromide (note moved to). Poly-L-lysine HBr (MW 70,000-150,000) is a positively charged amino acid polymer that serves as a non-specific attachment factor for cells. It promotes cell adhesion to solid substrates by enhancing electrostatic interactions between cell membranes and culture surfaces. The polymer is widely used to coat glass slides, coverslips, and culture plates to facilitate cell attachment. Poly-L-lysine is a research reagent and is not approved for clinical use. |
| Molecular Formula |
C18H38N6O4
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|---|---|
| Molecular Weight |
402.5321
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| Exact Mass |
226.032
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| CAS # |
25988-63-0
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| Related CAS # |
Poly-L-lysine hydrobromide (MW 30000-70000);25988-63-0;Poly-L-lysine hydrochloride;26124-78-7;Poly-L-lysine hydrobromide (MW 70000-150000);25988-63-0;Poly-L-lysine hydrobromide (MW ＞300000);25988-63-0
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| PubChem CID |
72363
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| Appearance |
White to off-white solid powder
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| Density |
1.156g/cm3
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| Boiling Point |
745.4ºC at 760mmHg
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| Flash Point |
404.6ºC
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| Vapour Pressure |
1.24E-05mmHg at 25°C
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| LogP |
1.886
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
17
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| Heavy Atom Count |
28
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| Complexity |
463
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C(CCN)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)N
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| InChi Key |
WBSCNDJQPKSPII-KKUMJFAQSA-N
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| InChi Code |
InChI=1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
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| Chemical Name |
(2S)-6-amino-2-[[(2S)-6-amino-2-[[(2S)-2,6-diaminohexanoyl]amino]hexanoyl]amino]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4843 mL | 12.4214 mL | 24.8429 mL | |
| 5 mM | 0.4969 mL | 2.4843 mL | 4.9686 mL | |
| 10 mM | 0.2484 mL | 1.2421 mL | 2.4843 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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