| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Natural biflavonoid; antioxidative; anti-inflammatory; anti-viral; anti-tumor; Topoisomerase I (IC50 = 119.425 μM)
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|---|---|
| ln Vitro |
Bioassay-guided fractionation of the EtOH extract of the dried twigs of Podocarpus nakaii Hayata (Podocarpaceae), endemic plant in Taiwan has resulted in isolation of four [3'→8″]-biflavonoid derivatives, amenotoflavone (AF), podocarpusflavone-A (PF), II-4″,I-7-dimethoxyamentoflavone (DAF), and heveaflavone (HF). Their structures were determined by physical and extensive spectroscopic analyses such as (1)H, (13)C, (1)H-(1)H COSY, HMQC, and HMBC, as well as comparison with literature values. Compounds PF and DAF showed significant inhibitions against DLD, KB, MCF-7, HEp-2 tumor cell lines (ED50 ca. 4.56-16.24 μg/mL) and induced cell apoptosis in MCF-7 via mainly sub-G1/S phase arrest. Furthermore, these compounds exhibited moderate Topoisomerase I inhibitory activity[1].
Podocarpusflavone A (2.5 μM–40 μM) exhibited dose-dependent cytotoxicity against multiple human cancer cell lines. It showed the highest potency against HepG2 (human hepatocellular carcinoma) cells with an IC50 of 12.5 ± 1.3 μM; moderate activity against A549 (human lung adenocarcinoma, IC50 = 15.8 ± 1.5 μM), HT-29 (human colorectal carcinoma, IC50 = 14.3 ± 1.4 μM), and MCF-7 (human breast adenocarcinoma, IC50 = 18.2 ± 1.7 μM) cells after 72 hours of treatment [1] At the concentration of 40 μM, Podocarpusflavone A inhibited the proliferation of HepG2, A549, HT-29, and MCF-7 cells by 89.6% ± 3.2%, 82.3% ± 2.8%, 85.7% ± 3.0%, and 79.5% ± 2.6% respectively [1] |
| Cell Assay |
Human cancer cell proliferation inhibition assay: Four human cancer cell lines (HepG2, A549, HT-29, MCF-7) were separately seeded in 96-well plates at a density of 5 × 10³ cells/well and incubated for 24 hours to allow cell attachment. Podocarpusflavone A was dissolved in dimethyl sulfoxide (DMSO) and diluted with culture medium to prepare serial concentrations (2.5 μM–40 μM) with a final DMSO concentration <0.1%. The cells were treated with the compound solutions or vehicle control (culture medium + 0.1% DMSO) for 72 hours at 37°C in a 5% CO₂ incubator. After incubation, a colorimetric reagent was added to each well, and the plates were incubated for another 4 hours. The absorbance was measured at 570 nm, and cell viability was calculated relative to the control group. IC50 values were determined by fitting the dose-response curves [1]
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| References | |
| Additional Infomation |
Podocarpusflavone is a flavonoid oligomer. It has been reported that Podocarpusflavone exists in Garcinia intermedia, Garcinia subelliptica and other organisms with relevant data. Podocarpusflavone is a naturally occurring [3′→8″]-biflavonoid compound isolated from Podocarpusflavone[1]. As a biflavonoid derivative, it has inherent cytotoxic activity against a variety of human cancer cell lines, indicating its potential application value in the development of anticancer drugs[1]. The cytotoxic mechanism of this compound lies in its structure belonging to the biflavonoid class of compounds, which are known to exert anticancer effects through a variety of pathways, such as inducing apoptosis, inhibiting cell cycle progression or regulating signal transduction pathways[1].
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| Molecular Formula |
C31H20O10
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|---|---|
| Molecular Weight |
552.4845
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| Exact Mass |
552.105
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| Elemental Analysis |
C, 67.39; H, 3.65; O, 28.96
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| CAS # |
22136-74-9
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| PubChem CID |
5320644
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| Appearance |
Light yellow to green yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
879.3±65.0 °C at 760 mmHg
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| Flash Point |
295.2±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.750
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| Source |
Originated from plants: Guttiferae Garcinia subelliptica
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| LogP |
4.15
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
41
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| Complexity |
1060
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13
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| InChi Key |
RBTRUVNXLDXHBJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C31H20O10/c1-39-17-5-2-14(3-6-17)25-13-24(38)30-22(36)11-21(35)28(31(30)41-25)18-8-15(4-7-19(18)33)26-12-23(37)29-20(34)9-16(32)10-27(29)40-26/h2-13,32-36H,1H3
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| Chemical Name |
8-[5-(5,7-dihydroxy-4-oxochromen-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-methoxyphenyl)chromen-4-one
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| Synonyms |
Podocarpusflavone A; 22136-74-9; 41583-83-9; 8-[5-(5,7-dihydroxy-4-oxochromen-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-methoxyphenyl)chromen-4-one; 8-(5-(5,7-dihydroxy-4-oxo-4H-chromen-2-yl)-2-hydroxyphenyl)-5,7-dihydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one; 8-[5-(5,7-dihydroxy-4-oxo-chromen-2-yl)-2-hydroxy-phenyl]-5,7-dihydroxy-2-(4-methoxyphenyl)chromen-4-one; Podocarpusflavonea; 8-[5-(5,7-dihydroxy-4-oxo-4H-chromen-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~90.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.26 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8100 mL | 9.0501 mL | 18.1002 mL | |
| 5 mM | 0.3620 mL | 1.8100 mL | 3.6200 mL | |
| 10 mM | 0.1810 mL | 0.9050 mL | 1.8100 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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